LOCUS       40924_25751        4505 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS374, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4505)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4505)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..481
                     /label=3' flanking his3 YIP-in fragment
                     /note="3' flanking his3 YIP-in fragment"
     rep_origin      complement(718..1306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1480..2337)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2338..2442)
                     /label=AmpR promoter
     promoter        2540..2755
                     /label=URA3 promoter
     CDS             2756..3556
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        4010..4197
                     /label=HIS3 promoter
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attccaggca tacgatatat atacatgtgt atatatgtat
      121 acctatgaat gtcagtaagt atgtatacga acagtatgat actgaagatg acaaggtaat
      181 gcatcattct atacgtgtca ttctgaacga ggcgcgcttt ccttttttct ttttgctttt
      241 tctttttttt tctcttgaac tcgactcctc ggcgcgccga ggagtcgaga aaaaaaatat
      301 aaaagagatg gaggaacggg aaaaagttag ttgtggtgat aggtggcaag tggtattccg
      361 taagaacaac aagaaaagca tttcatatta tggctgaact gagcgaacaa gtgcaaaatt
      421 taagcatcaa cgacaacaac gagaatggtt atgttcctcc tcacttaaga ggaggatctg
      481 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1141 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1441 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1501 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1561 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1621 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1681 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1741 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1801 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1861 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1921 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1981 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2041 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2101 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2161 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2221 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2281 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2341 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2401 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2461 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2521 cacgaggcca gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg
     2581 gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     2641 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     2701 agtattctta acccaactgc acagaacaaa aacatgcagg aaacgaagat aaatcatgtc
     2761 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     2821 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     2881 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     2941 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     3001 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     3061 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     3121 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     3181 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     3241 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     3301 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     3361 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     3421 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     3481 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     3541 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     3601 aattagagct tcaatttaat tatatcagtt attacccgcc ctttcgtctc gcgcgtttcg
     3661 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     3721 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     3781 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3841 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     3901 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccaggcgt
     3961 cgagcagtcc tttcccgcaa ttttcttttt ctattactct tggcctcctc tagtacactc
     4021 tatatttttt tatgcctcgg taatgatttt catttttttt tttccaccta gcggatgact
     4081 cttttttttt cttagcgatt ggcattatca cataatgaat tatacattat ataaagtaat
     4141 gtgatttctt cgaagaatat actaaaaaat gagcaggcaa gataaacgaa ggcaaagatg
     4201 acagagcaga aagccctagt aaagcgtatt acaaatgaaa ccaagattca gattgcgatc
     4261 tctttaaagg gtggtcccct agcgatagag cactcgatct tcccagaaaa agaggcagaa
     4321 gcagtagcag aacaggccac acaatcgcaa gtgattaacg tccacacagg tatagggttt
     4381 ctggaccata tgatacatgc tctggccaag cattccggct ggtcgctaat cgttgagtgc
     4441 attggtgact tacacataga cgaccatcac accactgaag actgcgggat tgctctcggt
     4501 ccctg
//
