LOCUS       40924_25746        4736 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS373, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..460
                     /label=3' flanking fcy1 YIP-in fragment
                     /note="3' flanking fcy1 YIP-in fragment"
     CDS             complement(863..1663)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1664..1879)
                     /label=URA3 promoter
     promoter        1977..2081
                     /label=AmpR promoter
     CDS             2082..2939
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3113..3701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3938..4736
                     /label=5' flanking fcy1 YIP-out fragment
                     /note="5' flanking fcy1 YIP-out fragment"
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attccagcat gtatggtatt ccacgctgtg ttgtcggtga
      121 gaacgttaat ttcaaaagta agggcgagaa atatttacaa actagaggtc acgaggttgt
      181 tgttgttgac gatgagaggt gtaaaaagat catgaaacaa tttatcgctc ctcggcgcgc
      241 cgaggagcga tgaaagacct caggattggt ttgaagatat tggtgagtag agcacgcagc
      301 acgctgtatt tacgtattta attttatata tttgtgcata cactactagg gaagacttga
      361 aaaaaaccta ggaaatgaaa aaacgacaca ggaagtcccg tatttactat tttttccttc
      421 cttttgatgg ggcagggcgg aaatagagga taggctcgac gcctggcgta atagcgaaga
      481 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      541 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      601 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      661 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      721 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      781 cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
      841 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
      901 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
      961 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     1021 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     1081 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     1141 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     1201 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     1261 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     1321 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     1381 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     1441 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     1501 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     1561 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     1621 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     1681 tgcatgtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     1741 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     1801 gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa
     1861 aaaaaaatga tgaattgaat tgaaaagctg gcctcgtgat acgcctattt ttataggtta
     1921 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2281 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2461 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2941 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3001 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3241 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3781 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3841 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3901 cgcctctccc cgcgcgttgg ccgattcatt aatgcaggca gatcccccaa gtcccagcgt
     3961 tccagtattc atactgcgcg ttagagtggt agccataaga gccggcatat tggtaatttt
     4021 cagtattaac gttagaacgt ggtgaatacg atgtggtcca gccttgcctc gttgtgtcat
     4081 atacgatctt tttctttggg tcacaaagaa tatcatatgc ttgagagatg actttaaatc
     4141 tatgtagttt ttcgcttgat gttagcagca gcggtgattt actatcactg ttggtaacct
     4201 tttctgagct aaatatttga atgttatcgg aatggtcagg gtggtacaat tttacataac
     4261 gatgatattt tttttttaac gacttcttgt ccagtttagg atttccagat ccggcctttg
     4321 gaatgccaaa aatatcatag ggagttggat ctgccaactc aggccattgt tcatccctta
     4381 tcgtaagttt tctattgcca tttttatcgt tcgctgtagc atacttagct ataaaagtga
     4441 tttgtggggg acacttttct acacatgata agtgccactt gaataaaaat gggtatacga
     4501 acttatggtg tagcataaca aatatattgc aagtagtgac ctatggtgtg tagatatacg
     4561 tacagttagt tacgagccta aagacacaac gtgtttgtta attatactgt cgctgtaata
     4621 tcttctcttc cattatcacc ggtcattcct tgcaggggcg gtagtacccg gagaccctga
     4681 acttttcttt ttttttttgc gaaattaaaa agttcatttt caattcgaca atgctg
//
