LOCUS       40924_25741        4725 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS112, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4725)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    86..407
                     /label=3' flanking ade8 YIP-in fragment
                     /note="3' flanking ade8 YIP-in fragment"
     CDS             complement(810..1610)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1611..1826)
                     /label=URA3 promoter
     promoter        1924..2028
                     /label=AmpR promoter
     CDS             2029..2886
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3060..3648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3888..4725
                     /label=5' flanking ade8 YIP-out fragment
                     /note="5' flanking ade8 YIP-out fragment"
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attcagccat tggtggtaaa aaagctagaa atcatacctg
      121 gcgaagaaac actagagcag tacgagcaaa gggtacacga tgctgagcac atcgctcctc
      181 ggcgcgccga ggagcgattg tagaagctac atataaggtt ttacagcagc ttcacaaata
      241 agaatagttc ccatatttat acttttatat ttggcattct tcaaagtgag gcgcgaaaca
      301 tctcgcgtcg taaatcatga tcatggattg tgacaaaacg atcttaaagg tttcgaataa
      361 aatgtgtgta aatacgtata cttatatatg aatgtgcatg tctcgacgcc tggcgtaata
      421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      481 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
      541 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      601 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      661 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
      721 gaaagggcgg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
      781 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
      841 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
      901 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
      961 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     1021 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     1081 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     1141 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     1201 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     1261 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     1321 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     1381 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     1441 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     1501 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     1561 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     1621 cgtttcctgc atgtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     1681 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     1741 tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa
     1801 gaataaaaaa aaaatgatga attgaattga aaagctggcc tcgtgatacg cctattttta
     1861 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1921 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1981 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2041 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2101 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2161 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2221 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2281 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcagat cctaaacagg
     3901 caaaggttca ttctttctga tccgttctat cagaaattta acggcttgaa ctctataagg
     3961 atcagacttg cctactacta aagcgttctg caagaaattc ctgataaggt cctgtagaac
     4021 ggccttgcga ccggaaaccg ggaaagatac agacctgcaa atatccttca attcggacac
     4081 tttcagcagt tccatcagag tgatggtttc ctccacctcg tttcttagtt cattagtcgg
     4141 ttccctatca ggcccaggcg tttcatcttc ccagtaatcc tctaaattta tcattgaaac
     4201 agaatgtata tggaacactc aactgtttga gttggagtct tcttttcttg tgaaatatgg
     4261 atgaactcca cttcattctt tcatattcat attaaagatg atacactttt caacttacta
     4321 tctggtgcgg tgaatgggtg tatacattga ctacagctca ggtgacatct aggggccgtt
     4381 tagtgtaaga atataaagta caatccaaaa aggaaaatta tacttgttta agaaaagagg
     4441 aaccttctgc tcgctctaaa atggtagttc tagtgatagt ttaaaaatgc attttccttc
     4501 cgacttcgaa gatagatttc agcataaaga ctaaaagcgt cagcaaggat tcgaatgagt
     4561 gactcgttgc tagtgactct gacctgtttt ttattccagc aagaggaaag ttatatagag
     4621 tagtttatga agagaagaca cttctaaagt tgaataacgc tcctagcctc aaggttgatg
     4681 atttaacttg cagcaagcgc aggtgagagc caacacacat gaatt
//
