LOCUS       V013171                10697 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013171
VERSION     V013171
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             924..4040
                     /gene="ITGA2B"
                     /label="Integrin alpha-IIb"
                     /note="Integrin alpha-IIb from Homo sapiens. Accession#:
                     P08514"
     CDS             4041..4094
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4107..6470
                     /gene="ITGB3"
                     /label="Integrin beta-3"
                     /note="Integrin beta-3 from Homo sapiens. Accession#:
                     P05106"
     intron          6577..6806
                     /label="chimeric intron"
                     /note="chimera between introns from adenovirus and
                     immunoglobulin heavy chain genes"
     misc_feature    6879..7465
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             7485..8081
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    8318..8439
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8588..8618)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8633..8654)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8942..9530)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9704..10561)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10562..10666)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatcgcc accatggcca gagctttgtg tccactgcaa gccctctggc
      961 ttctggagtg ggtgctgctg ctcttgggac cttgtgctgc ccctccagcc tgggccttga
     1021 acctggaccc agtgcagctc accttctatg caggccccaa tggcagccag tttggatttt
     1081 cactggactt ccacaaggac agccatggga gagtggccat cgtggtgggc gccccgcgga
     1141 ccctgggccc cagccaggag gagacgggcg gcgtgttcct gtgcccctgg agggccgagg
     1201 gcggccagtg cccctcgctg ctctttgacc tccgtgatga gacccgaaat gtaggctccc
     1261 aaactttaca aaccttcaag gcccgccaag gactgggggc gtcggtcgtc agctggagcg
     1321 acgtcattgt ggcctgcgcc ccctggcagc actggaacgt cctagaaaag actgaggagg
     1381 ctgagaagac gcccgtaggt agctgctttt tggctcagcc agagagcggc cgccgcgccg
     1441 agtactcccc ctgtcgcggg aacaccctga gccgcattta cgtggaaaat gattttagct
     1501 gggacaagcg ttactgtgaa gcgggcttca gctccgtggt cactcaggcc ggagagctgg
     1561 tgcttggggc tcctggcggc tattatttct taggtctcct ggcccaggct ccagttgcgg
     1621 atattttctc gagttaccgc ccaggcatcc ttttgtggca cgtgtcctcc cagagcctct
     1681 cctttgactc cagcaaccca gagtacttcg acggctactg ggggtactcg gtggccgtgg
     1741 gcgagttcga cggggatctc aacactacag aatatgtcgt cggtgccccc acttggagct
     1801 ggaccctggg agcggtggaa attttggatt cctactacca gaggctgcat cggctgcgcg
     1861 gagagcagat ggcgtcgtat tttgggcatt cagtggctgt cactgacgtc aacggggatg
     1921 ggaggcatga tctgctggtg ggcgctccac tgtatatgga gagccgggca gaccgaaaac
     1981 tggccgaagt ggggcgtgtg tatttgttcc tgcagccgcg aggcccccac gcgctgggtg
     2041 cccccagcct cctgctgact ggcacacagc tctatgggcg attcggctct gccatcgcac
     2101 ccctgggcga cctcgaccgg gatggctaca atgacattgc agtggctgcc ccctacgggg
     2161 gtcccagtgg ccggggccaa gtgctggtgt tcctgggtca gagtgagggg ctgaggtcac
     2221 gtccctccca ggtcctggac agccccttcc ccacaggctc tgcctttggc ttctcccttc
     2281 gaggtgccgt agacatcgat gacaacggat acccagacct gatcgtggga gcttacgggg
     2341 ccaaccaggt ggctgtgtac agagctcagc cagtggtgaa ggcctctgtc cagctactgg
     2401 tgcaagattc actgaatcct gctgtgaaga gctgtgtcct acctcagacc aagacacccg
     2461 tgagctgctt caacatccag atgtgtgttg gagccactgg gcacaacatt cctcagaagc
     2521 tatccctaaa tgccgagctg cagctggacc ggcagaagcc ccgccagggc cggcgggtgc
     2581 tgctgctggg ctctcaacag gcaggcacca ccctgaacct ggatctgggc ggaaagcaca
     2641 gccccatctg ccacaccacc atggccttcc ttcgagatga ggcagacttc cgggacaagc
     2701 tgagccccat tgtgctcagc ctcaatgtgt ccctaccgcc cacggaggct ggaatggccc
     2761 ctgctgtcgt gctgcatgga gacacccatg tgcaggagca gacacgaatc gtcctggact
     2821 gtggggaaga tgacgtatgt gtgccccagc ttcagctcac tgccagcgtg acgggctccc
     2881 cgctcctagt tggggcagat aatgtcctgg agctgcagat ggacgcagcc aacgagggcg
     2941 agggggccta tgaagcagag ctggccgtgc acctgcccca gggcgcccac tacatgcggg
     3001 ccctaagcaa tgtcgagggc tttgagagac tcatctgtaa tcagaagaag gagaatgaga
     3061 ccagggtggt gctgtgtgag ctgggcaacc ccatgaagaa gaacgcccag ataggaatcg
     3121 cgatgttggt gagcgtgggg aatctggaag aggctgggga gtctgtgtcc ttccagctgc
     3181 agatacggag caagaacagc cagaatccaa acagcaagat tgtgctgctg gacgtgccgg
     3241 tccgggcaga ggccccagtg gagctgcgag ggaactcctt tccagcctcc ctggtggtgg
     3301 cagcagaaga aggtgagagg gagcagaaca gcttggacag ctggggaccc aaagtggagc
     3361 acacctatga gctccacaac aatggccctg ggactgtgaa tggtcttcac ctcagcatcc
     3421 accttccggg acagtcccag ccctccgacc tgctctacat cctggatata cagccccagg
     3481 ggggccttca gtgcttccca cagcctcctg tcaaccctct caaggtggac tgggggctgc
     3541 ccatccccag cccctccccc attcacccgg cccatcacaa gcgggatcgc agacagatct
     3601 tcctgccaga gcccgagcag ccctcgaggc ttcaggatcc agttctcgta agctgcgact
     3661 cggcgccctg tactgtggtg cagtgtgacc tgcaggagat ggcgcgcggg cagcgggcca
     3721 tggtcacggt gctggccttc ctgtggctgc ccagcctcta ccagaggcct ctggatcagt
     3781 ttgtgctgca gtcgcacgca tggttcaacg tgtcctccct cccctatgcg gtgcccccgc
     3841 tcagcctgcc ccgaggggaa gctcaggtgt ggacacagct gctccgggcc ttggaggaga
     3901 gggccattcc aatctggtgg gtgctggtgg gtgtgctggg tggcctgctg ctgctcacca
     3961 tcctggtcct ggccatgtgg aaggtcggct tcttcaagcg gaaccggcca cccctggaag
     4021 aagatgatga agagggggag gagggcagag gaagtcttct aacatgcggt gacgtggagg
     4081 agaatcccgg ccctgctagc gccaccatgc gagcgcggcc gcggccccgg ccgctctggg
     4141 cgactgtgct ggcgctgggg gcgctggcgg gcgttggcgt aggagggccc aacatctgta
     4201 ccacgcgagg tgtgagctcc tgccagcagt gcctggctgt gagccccatg tgtgcctggt
     4261 gctctgatga ggccctgcct ctgggctcac ctcgctgtga cctgaaggag aatctgctga
     4321 aggataactg tgccccagaa tccatcgagt tcccagtgag tgaggcccga gtactagagg
     4381 acaggcccct cagcgacaag ggctctggag acagctccca ggtcactcaa gtcagtcccc
     4441 agaggattgc actccggctc cggccagatg attcgaagaa tttctccatc caagtgcggc
     4501 aggtggagga ttaccctgtg gacatctact acttgatgga cctgtcttac tccatgaagg
     4561 atgatctgtg gagcatccag aacctgggta ccaagctggc cacccagatg cgaaagctca
     4621 ccagtaacct gcggattggc ttcggggcat ttgtggacaa gcctgtgtca ccatacatgt
     4681 atatctcccc accagaggcc ctcgaaaacc cctgctatga tatgaagacc acctgcttgc
     4741 ccatgtttgg ctacaaacac gtgctgacgc taactgacca ggtgacccgc ttcaatgagg
     4801 aagtgaagaa gcagagtgtg tcacggaacc gagatgcccc agagggtggc tttgatgcca
     4861 tcatgcaggc tacagtctgt gatgaaaaga ttggctggag gaatgatgca tcccacttgc
     4921 tggtgtttac cactgatgcc aagactcata tagcattgga cggaaggctg gcaggcattg
     4981 tccagcctaa tgacgggcag tgtcatgttg gtagtgacaa tcattactct gcctccacta
     5041 ccatggatta tccctctttg gggctgatga ctgagaagct atcccagaaa aacatcaatt
     5101 tgatctttgc agtgactgaa aatgtagtca atctctatca gaactatagt gagctcatcc
     5161 cagggaccac agttggggtt ctgtccatgg attccagcaa tgtcctccag ctcattgttg
     5221 atgcttatgg gaaaatccgt tctaaagtag agctggaagt gcgtgacctc cctgaagagt
     5281 tgtctctatc cttcaatgcc acctgcctca acaatgaggt catccctggc ctcaagtctt
     5341 gtatgggact caagattgga gacacggtga gcttcagcat tgaggccaag gtgcgaggct
     5401 gtccccagga gaaggagaag tcctttacca taaagcccgt gggcttcaag gacagcctga
     5461 tcgtccaggt cacctttgat tgtgactgtg cctgccaggc ccaagctgaa cctaatagcc
     5521 atcgctgcaa caatggcaat gggacctttg agtgtggggt atgccgttgt gggcctggct
     5581 ggctgggatc ccagtgtgag tgctcagagg aggactatcg cccttcccag caggacgaat
     5641 gcagcccccg ggagggtcag cccgtctgca gccagcgggg cgagtgcctc tgtggtcaat
     5701 gtgtctgcca cagcagtgac tttggcaaga tcacgggcaa gtactgcgag tgtgacgact
     5761 tctcctgtgt ccgctacaag ggggagatgt gctcaggcca tggccagtgc agctgtgggg
     5821 actgcctgtg tgactccgac tggaccggct actactgcaa ctgtaccacg cgtactgaca
     5881 cctgcatgtc cagcaatggg ctgctgtgca gcggccgcgg caagtgtgaa tgtggcagct
     5941 gtgtctgtat ccagccgggc tcctatgggg acacctgtga gaagtgcccc acctgcccag
     6001 atgcctgcac ctttaagaaa gaatgtgtgg agtgtaagaa gtttgaccgg ggagccctac
     6061 atgacgaaaa tacctgcaac cgttactgcc gtgacgagat tgagtcagtg aaagagctta
     6121 aggacactgg caaggatgca gtgaattgta cctataagaa tgaggatgac tgtgtcgtca
     6181 gattccagta ctatgaagat tctagtggaa agtccatcct gtatgtggta gaagagccag
     6241 agtgtcccaa gggccctgac atcctggtgg tcctgctctc agtgatgggg gccattctgc
     6301 tcattggcct tgccgccctg ctcatctgga aactcctcat caccatccac gaccgaaaag
     6361 aattcgctaa atttgaggaa gaacgcgcca gagcaaaatg ggacacagcc aacaacccac
     6421 tgtataaaga ggccacgtct accttcacca atatcacgta ccggggcact taaaccggtc
     6481 gtacgtctcc ggattcgaat tcggatccgc ggccgcatag ataactgatc cagtgtgctg
     6541 gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg
     6601 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     6661 ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt
     6721 tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga
     6781 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca
     6841 tgcatctagg gcggccaatt ccgcccctct cccccccccc cctctccctc ccccccccct
     6901 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6961 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     7021 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7081 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7141 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7201 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7261 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     7321 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     7381 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     7441 acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc
     7501 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     7561 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     7621 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7681 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7741 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7801 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7861 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7921 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7981 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8041 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     8101 cgcagcgccc gaccgaaagg agcgcacgac cccatggctc cgaccgaagc cacccggggc
     8161 ggccccgccg accccgcacc cgcccccgag gcccaccgac tctagataac tgatcataat
     8221 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8281 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8341 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8401 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgctcgagtg cattctagtt
     8461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8521 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8581 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8641 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8701 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8761 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8821 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8881 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8941 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9001 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9061 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9121 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9181 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9241 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9301 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9361 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9421 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9481 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9541 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9601 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9661 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9721 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9781 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9841 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9901 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9961 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10021 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10081 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10141 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10201 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10261 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10321 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10381 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10441 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10501 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10561 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10621 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10681 aagtgccacc tgacgtc
//
