LOCUS       40924_25656       10070 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pIREB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM, 
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both 
            exploiting and eluding DNA double-strand break repair systems in 
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
  PUBMED    23251422
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of 
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 10070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(2028..3927)
                     /direction=LEFT
                     /label=mycobacterial OriM region from pAL5000
                     /note="mycobacterial OriM region from pAL5000"
     CDS             4201..7245
                     /label=lacZ
                     /note="beta-galactosidase"
     repeat_region   8411..8450
                     /rpt_family="IR-1"
     promoter        8496..8524
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             8713..9243
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     repeat_region   9343..9378
                     /rpt_family="IR-2"
     terminator      complement(9991..10034)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcacgcgtg agcccaccag
     2041 ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     2101 cgggtctaag gcggcgtgta cggccgccac agcggctctt agcggcccgg aaacgtcctc
     2161 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     2221 ggtgtttcat catcagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     2281 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     2341 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     2401 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     2461 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     2521 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     2581 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     2641 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     2701 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     2761 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     2821 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     2881 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     2941 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     3001 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     3061 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     3121 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     3181 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     3241 cgcgcaaagt ccgcgtcagc catgcatgga ggcattgcta tgagcgacgg ctacagcgac
     3301 ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc
     3361 gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag
     3421 gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac
     3481 gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc
     3541 gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa
     3601 aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc
     3661 gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac
     3721 gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg tccgttttga
     3781 ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg
     3841 aatatcgtgg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt
     3901 tgtagtgctt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagacg
     3961 acacatgccc agacactgcg gaaatgccac cttcaggccg tcgcgtcggt cccgaattgg
     4021 ccgtgaacga ccgccggata agggtttcgg cggtgcgctt gatgcgggtg gacgcccgaa
     4081 gttgtggttg actacacgag cactgccggg cccagcgcct gcagtctgac ctaattcagg
     4141 atgcgcccaa acatgcatgg atgcgttgag atgaggatga gggaagcaag aatggatccc
     4201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4321 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     4381 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
     4441 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     4501 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     4561 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
     4621 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
     4681 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
     4741 ctgcgttgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
     4801 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
     4861 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
     4921 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
     4981 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
     5041 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
     5101 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
     5161 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
     5221 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
     5281 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
     5341 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
     5401 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
     5461 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
     5521 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
     5581 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
     5641 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
     5701 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
     5761 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
     5821 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
     5881 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
     5941 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
     6001 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
     6061 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
     6121 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
     6181 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
     6241 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
     6301 tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
     6361 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
     6421 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
     6481 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
     6541 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
     6601 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
     6661 acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
     6721 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
     6781 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
     6841 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
     6901 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
     6961 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
     7021 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
     7081 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
     7141 ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag
     7201 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc
     7261 aggggggatc cttgccgagc tgggatggaa gcccggccga cccaccctgg aggagatgat
     7321 cgaggatgcc agggcctttc acgcccgccg ctgctgagcg tccgccgccg ggcccgcacc
     7381 gccgtcggcc ggcccgctcc gggctcgcag cagcgggctt cggcgcgggc ccggggctcc
     7441 cgggccgccg ggcggggctc cgcccggcgg ccgccggggg ccgggggcgg cgccgggcgg
     7501 cccggggcgt caggcgccgg gggcggtgtc cggcggcccc cagaggaact gcgccagttc
     7561 ctccggatcg gtgaagccgg agagatccag cggggtctcc tcgaacacct cgaagtcgtg
     7621 caggaaggtg aaggcgagca gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc
     7681 gtcgagcagc gcggccagga tctcgcggtc gccccggaag gcgttgagat gcagttgcac
     7741 caggctgtag cgggagtctc ccgcatagac gtcggtgaag tcgacgatcc cggtgacctc
     7801 ggtcgcggcc aggtccacga agatgttggt cccgtgcagg tcgccgtgga cgaaccgggg
     7861 ttcgcggccg gccagcagcg tgtccacgtc cggcagccag tcctccaggc ggtccagcag
     7921 ccggggcgag aggtagcccc acccgcggtg gtcctcgacg gtcgccgcgc ggcgttcccg
     7981 cagcagttcc gggaagacct cggaatgggg ggtgagcacg gtgttcccgg tcagcggcac
     8041 cctgtgcagc cggccgagca cccggccgag ttcgcgggcc agggcgagca gcgcgttccg
     8101 gtcggtcgtg ccgtccatcg cggaccgcca ggtggtgccg gtcatccggc tcatcaccag
     8161 gtagggccac ggccaggctc cggtgccggg ccgcagctcg ccgcggccga ggaggcgggg
     8221 caccggcacc ggggcgtccg ccaggaccgc gtacgcctcc gactccgacg cgaggctctc
     8281 cggaccgcac cagtgctcgc cgaacagctt gatcaccggg tcgggctcgc cgaccagtac
     8341 ggggttggtg ctctcgccgg gcacccgcag caccggcggc accggcagcc cgagctcctc
     8401 cagggctcgg cgggccagcg gctcccagaa ttctgggagc cgctggcccg ttcgatgttt
     8461 aaacaacggt gacctaagtg ggtaccgagc tcgaattgac ataagcctgt tcggttcgta
     8521 aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     8581 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt
     8641 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     8701 ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa
     8761 acaaagttag gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa
     8821 gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc
     8881 tactcccaac atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc
     8941 atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg
     9001 cccaggtttg agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag
     9061 caccggaggc agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg
     9121 cttggtgctt atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc
     9181 tatacaaagt tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc
     9241 acctaacaat tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg
     9301 tcacaacgcc gcggccaatt cgagctcggt acccactgct tgctgcggaa cgaccaggaa
     9361 ttcctggtcg ttccgcaggc tcgcgtagga atcatccgaa tcaatacggt cgagaagtaa
     9421 cagggattct tgtgtcacag cggacctcta ttcacagggt acgggccggc ttaattccgc
     9481 acggccggtc gcgacacggc ctgtccgcac cgcggatcag gcgttgacga tgacgggctg
     9541 gtcggccacg tcggggacgt tctcggtggt gctgcggtcg ggatcgccaa tctctacggg
     9601 ccgaccgagg cgacggtgta cgccaccgcc tggttctgcg acggcgaggc gccgtcccag
     9661 gccccgccga tccccgtccc ccgcgtcgtc gagcgcggtg ccgacgacac cgccgcgtgg
     9721 ctcgtcacgg aggccgtccc cggcgtcgcg gcggccgagg agtggcccga gcaccagcgg
     9781 ttcgccgtgg tcgaggcgat ggcggagctg gcccgcgccc tccacgagct gcccgtggag
     9841 gactgcccct tcgaccggcg cctcgacgcg gcggtcgccg aggcccggcg gaacgtcgcc
     9901 gagggcctgt ggacctcgac gacctgcagg catgcaagct tatcgatgtc gacgtagtta
     9961 actagcgtac gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
    10021 gcctttcgtt ttatctgttg tttgtccggc catcatggcc gcggtgatca
//
