LOCUS       40924_25636        7192 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pIPKTA30N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Douchkov D, Nowara D, Zierold U, Schweizer P.
  TITLE     A high-throughput gene-silencing system for the functional 
            assessment of defense-related genes in barley epidermal cells
  JOURNAL   Mol. Plant Microbe Interact. 18 (8), 755-761 (2005)
  PUBMED    16134887
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Douchkov D, Schweizer P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2007) Cytogenetics, Institute of Plant Genetics 
            and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben 
            D-06466, Germany
REFERENCE   3  (bases 1 to 7192)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7192)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2005"; volume: "18"; issue: "8"; pages: 
            "755-761"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-2007) Cytogenetics, Institute of Plant Genetics and Crop 
            Plant Research (IPK), Corrensstrasse 3, Gatersleben D-06466, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        432..777
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    791..915
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        940..970
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1024..1680
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2025..2327
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2371..2495)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2508..2838
                     /number=2
                     /note="RGA2"
     protein_bind    2841..2965
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3009..3311)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3656..4312)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4366..4396)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4421..4545)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      4549..4773
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     primer_bind     complement(4796..4812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5286..5390
                     /label=AmpR promoter
     CDS             5391..6248
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6422..7010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccatg
      241 cctcgaggca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
      301 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
      361 atggtggagc acgacacgct tgtctactcc aaaaatatca aagatacagt ctcagaagac
      421 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
      481 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
      541 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
      601 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      661 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
      721 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacaggg
      781 tacccccatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
      841 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      901 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
      961 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct
     1021 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1081 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1141 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1201 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1261 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1321 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1381 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1441 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1501 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1561 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     1621 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1681 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     1741 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg
     1801 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     1861 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     1921 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     1981 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     2041 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     2101 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2161 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2221 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     2281 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     2341 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     2401 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2461 tacgtttctc gttcagcttt cttgtacaaa gtggtgatgg gggatcctct agaaaaggta
     2521 tggatagtgt atcttcatac tgcatttgtt taatttgaaa atggttatct agttgcctaa
     2581 caaaatatag ctgggatatc ttataacaca tgtgcaggtg acatggaaaa aaatgcctat
     2641 ttttctatgc actaactatt catcatgtga catacttccc caaaaaacta aataagccaa
     2701 attttccagc ttccgagtcc tgaaaaagag tagtgtacct gatacaattt atagagtttt
     2761 ttttttcgaa aagaagggat agccctcata gatagagtac taactaaaag tctactttta
     2821 ccaatttcag gtttttgatc accactttgt acaagaaagc tgaacgagaa acgtaaaatg
     2881 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     2941 aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat aagggagcct
     3001 gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg
     3061 ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc
     3121 atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta
     3181 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
     3241 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
     3301 ttaaactgca tttcaccagt ccctgttctc gtcagcaaaa gagccgttca tttcaataaa
     3361 ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg
     3421 acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc
     3481 ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcacac
     3541 ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag
     3601 cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatccacgc gtttacgccc
     3661 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     3721 catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     3781 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     3841 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     3901 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     3961 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     4021 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     4081 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     4141 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     4201 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     4261 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     4321 ccttagctcc tgaaaatctc gccggatcct aactcaaaat ccacacatta tacgagccgg
     4381 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
     4441 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     4501 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tagtgtcgac
     4561 ctgcaggcat gccgctgaaa tcaccagtct ctctctacaa atctatctct ctctataata
     4621 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     4681 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     4741 ttctaattcc taaaaccaaa atccagtggg taccgagctc gaattcaagc ttggcactgg
     4801 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4861 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4921 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     4981 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     5041 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5101 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5161 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5221 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5281 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5341 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5401 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5461 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5521 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5581 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5641 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5701 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5761 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5821 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5881 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5941 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6001 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6061 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6541 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7081 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7141 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
