LOCUS       40924_25601        4887 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pINV-110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2005) Electrical Engineering, Princeton 
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4887)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005"; volume: "434"; issue: "7037"; pages: "1130-1134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2005) Electrical Engineering, Princeton University, B-312, 
            E-Quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(22..1101)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1102..1179)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1245..1266
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1281..1311
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1319..1335
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1367..1378
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1385..2095
                     /label=lambda repressor
                     /note="phage lambda repressor"
     RBS             2156..2167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      2156..2164
                     /gene="ecfP"
                     /regulatory_class="ribosome_binding_site"
     CDS             2174..2890
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2917..3003
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3173..3718)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3832..3926)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3960..4751)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtcattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
       61 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
      121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
      181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
      241 ggttgacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
      301 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
      361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
      421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
      481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
      541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
      601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
      661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
      721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
      781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
      841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
      901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
      961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     1021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     1081 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     1141 tgccataccg cgaaaggttt tgcaccattc gatggtgtca acgtaatcta gagctagcac
     1201 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1261 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1321 gtgagcggat aacaatggat aacaatttca cacaggggta ccaattaaag aggagaaatt
     1381 aagcatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
     1441 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
     1501 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
     1561 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aattcagccc
     1621 ttcaatcgcc agagagatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
     1681 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct
     1741 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
     1801 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
     1861 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agcccgggga
     1921 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
     1981 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
     2041 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctgatc
     2101 ggcaaggtgt tctggtcggc gcatagctga taacaattgg tacctagaat tgcttaaaga
     2161 ggagaaatta agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2221 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2281 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2341 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
     2401 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2461 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2521 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2581 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
     2641 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     2701 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2761 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2821 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2881 gctgtacaag taatcggccg tctcgagtag aggcatcaaa taaaacgaaa ggctcagtcg
     2941 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     3001 aatccgccgc cctagaccta ggggatatat tccgcttcct cgctcactga ctcgctacgc
     3061 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     3121 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     3181 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     3241 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     3301 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     3361 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     3421 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     3481 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     3541 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     3601 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     3661 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     3721 agatcatctt attaatcaga taaaatatta ctagatttca gtgcaattta tctcttcaaa
     3781 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     3841 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     3901 cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag
     3961 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4021 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4081 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     4141 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4201 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     4261 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4321 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4381 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4441 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4501 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4561 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4621 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4681 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4741 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc
     4801 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     4861 ccagagggcg ccccagctgg caattcc
//
