LOCUS       40924_25596        6394 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 6394)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "761-766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2006) Biopathology, University of Palermo, via Divisi, 83, 
            Palermo 90100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    59..290
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        535..942
                     /label=LEU2 promoter
     CDS             943..2034
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2694..3024)
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     CDS             3065..3781
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3811..4080
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        4488..4592
                     /label=AmpR promoter
     CDS             4593..5450
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5624..6212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagatcgtc
       61 gacatggaaa gtgatgttga agatgacgat aaagaaaccc aactaaagaa aagggctgta
      121 agcggtgtag aattgcgtat ttatgtttaa tatgtcatac ttttagcgct cattgttatt
      181 gtatactatt tatagctaac agtttattat tagatttcct ctaataagcg ttataccagt
      241 gtagtaagtt tatcacttca gctatgtgtc attttttttt tttttttttg aggatcgagg
      301 agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg gaatcccaac
      361 aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt
      421 gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt
      481 tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt
      541 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
      601 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
      661 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
      721 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
      781 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
      841 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
      901 tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag
      961 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
     1021 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
     1081 ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc
     1141 aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt
     1201 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
     1261 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
     1321 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
     1381 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
     1441 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
     1501 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
     1561 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct
     1621 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
     1681 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
     1741 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
     1801 tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg
     1861 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
     1921 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
     1981 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
     2041 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
     2101 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
     2161 catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga
     2221 tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg
     2281 aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat
     2341 ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat
     2401 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
     2461 aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag tcgatcctca
     2521 aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta
     2581 tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc
     2641 caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt gttcggagca
     2701 gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg
     2761 aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtacttcaat
     2821 atagcaatga gcagttaagc gtattactga aagttccaaa gagaaggttt ttttaggcta
     2881 agataatggg gctctttaca tttccacaac atataagtaa gattagatat ggatagttat
     2941 atggatatgt atatggtggt aatgccatgt aatatgatta ttaaacttct ttgcgtccat
     3001 ccaaaaaaaa agtaagaatt tttgctcgac ggtaccgcgg gcccgggatc caccggtcgc
     3061 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3121 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3181 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3241 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3301 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3361 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3421 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3481 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3541 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3601 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3661 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3721 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3781 gtaaagcggc cgcgactcta gcttgatcct tcttttgtga aaaggagtat atttatataa
     3841 ctgaataaaa atatatattg tgacaaatgc ccatgcaaaa aatcgtgaaa aaaggaagaa
     3901 agttctacta ttctatgtaa aaataaaccc attagatttt tttcctcttt gtgtaatgct
     3961 cactaccgcg attatcgttc gtttcatctg gacatgccaa cgttcttgac cttttagaac
     4021 ttacgaccgg atgaatactg tcatcatcac atgcaagtaa gtttttggtc ataaagtcga
     4081 cgatctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4141 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4201 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4261 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4321 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4381 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4441 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4801 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5761 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6361 agcgcagcga gtcagtgagc gaggaagcgg aaga
//
