LOCUS       40924_25586        5637 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 5637)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5637)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "761-766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2006) Biopathology, University of Palermo, via Divisi, 83, 
            Palermo 90100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    59..280
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        535..942
                     /label=LEU2 promoter
     CDS             943..2034
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2694..3024)
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     misc_feature    3025..3323
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        3731..3835
                     /label=AmpR promoter
     CDS             3836..4693
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4867..5455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagatcgtc
       61 gacatggaaa gtgatgttga agatgacgat aaagaaaccc aactaaagaa aagggctgta
      121 agcggtgtag aattgcgtat ttatgtttaa tatgtcatac ttttagcgct cattgttatt
      181 gtatactatt tatagctaac agtttattat tagatttcct ctaataagcg ttataccagt
      241 gtagtaagtt tatcacttca gctatgtgtc attttttttt tttttttttg aggatcgagg
      301 agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg gaatcccaac
      361 aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt
      421 gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt
      481 tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt
      541 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
      601 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
      661 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
      721 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
      781 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
      841 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
      901 tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag
      961 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
     1021 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
     1081 ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc
     1141 aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt
     1201 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
     1261 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
     1321 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
     1381 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
     1441 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
     1501 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
     1561 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct
     1621 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
     1681 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
     1741 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
     1801 tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg
     1861 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
     1921 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
     1981 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
     2041 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
     2101 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
     2161 catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga
     2221 tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg
     2281 aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat
     2341 ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat
     2401 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
     2461 aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag tcgatcctca
     2521 aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta
     2581 tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc
     2641 caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt gttcggagca
     2701 gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg
     2761 aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtacttcaat
     2821 atagcaatga gcagttaagc gtattactga aagttccaaa gagaaggttt ttttaggcta
     2881 agataatggg gctctttaca tttccacaac atataagtaa gattagatat ggatagttat
     2941 atggatatgt atatggtggt aatgccatgt aatatgatta ttaaacttct ttgcgtccat
     3001 ccaaaaaaaa agtaagaatt tttgctcgag aattccccgg gaagcttgat ccttcttttg
     3061 tgaaaaggag tatatttata taactgaata aaaatatata ttgtgacaaa tgcccatgca
     3121 aaaaatcgtg aaaaaaggaa gaaagttcta ctattctatg taaaaataaa cccattagat
     3181 ttttttcctc tttgtgtaat gctcactacc gcgattatcg ttcgtttcat ctggacatgc
     3241 caacgttctt gaccttttag aacttacgac cggatgaata ctgtcatcat cacatgcaag
     3301 taagtttttg gtcataaagt cgacgatctg gcgtaatagc gaagaggccc gcaccgatcg
     3361 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     3421 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     3481 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3541 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3601 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3661 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3721 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3781 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3841 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3901 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3961 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4021 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4081 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4141 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4201 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4261 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4321 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4381 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4441 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4501 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4561 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4621 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4681 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4741 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4801 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4981 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5041 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5101 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5161 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5221 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5281 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5341 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5401 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5461 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5521 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5581 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//
