LOCUS       40924_25581        4240 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 4240)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "761-766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2006) Biopathology, University of Palermo, via Divisi, 83, 
            Palermo 90100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..306
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     misc_feature    314..1225
                     /label=SUP16
                     /note="SUP16"
     misc_feature    1230..1927
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        2334..2438
                     /label=AmpR promoter
     CDS             2439..3296
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3470..4058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagatcgtc
       61 gacatggaaa gtgatgttga agatgacgat aaagaaaccc aactaaagaa aagggctgta
      121 agcggtgtag aattgcgtat ttatgtttaa tatgtcatac ttttagcgct cattgttatt
      181 gtatactatt tatagctaac agtttattat tagatttcct ctaataagcg ttataccagt
      241 gtagtaagtt tatcacttca gctatgtgtc attttttttt tttttttttg aggccttata
      301 tgaaaataag cttatcgata tcctgttgta gctttctttg agacatgaca gaatgagcgg
      361 atgtttcatg caatcaataa aaaaatatac taatagaggt tcaattacag ttcctaatta
      421 tttattgacg cggaagggac gctgtttgat cttgttatta tctaaattag ttcattgcac
      481 caaaaatgtg gaagtacttt tatgctataa aaagtttcgt agttagaatt ggtggattgt
      541 cgaaaaatca ataaagaagg gtaatacaat gttttgcatc acatgaccat gttcgagcaa
      601 tttatggcgc catataacaa gcacaaaaaa atgggtaaag tatggtatgt ttagacaagc
      661 tgatatgtta ccgtatgact ttcgtagcaa gttgccatat tggtccatga cctcttgagt
      721 gattttgaga tatgtgtgtt tgtataattg taaaagaacc tggtgctgac atggtggtta
      781 ttgaaaaata atttcaaaaa ataatacgac accagcagga tttgaaccag cgcgggcaga
      841 gcccaacaga tttcaagtct gtcgccttaa ccactcggcc atagtgccca aaataatctg
      901 gtagtgttag aaaacggaag gtattaaaaa catctgacta aaattaattt ccttatattg
      961 atgatgagtg tttcactagc agaagtaatt ctagctaggg acactaagag tgttggaatg
     1021 aatattatat attatttttt gactattatt catattactg ttacatattg tcatatacga
     1081 tgcaagaaga tgacataaag attgggaagt agtcgccaaa ttttttattg aagttaaagt
     1141 gtaatgattg gaacttctag catattctgt atatataata ttatcgacta tggaatccca
     1201 acaattttac aaaattctcc agatcaattc acatttatca ttgttggcga ttgttgattc
     1261 gtggtttcaa cttggtattg atccatatga tcatatgatt tgttcgacgc cattattcag
     1321 aatattcctg ggtgtgtccg aatatctttt tttaaaacct ggcttattag aacagacaat
     1381 tgatgcagct atccatgata aatcattcat aaaagatgat ttagaatttg ttatttcggc
     1441 tagagaatgg agctcggttg tttcttttgc caattttgat atatacaaaa agcaatttca
     1501 gagtgttcaa aagttctttg agccaatgct tccagaggct aatctcattg gcaacgagat
     1561 gataaaaacc attctgagtc attctagtga ccgttcggcc gctgaaaaaa gaaatacata
     1621 atgtttttaa tgctgatttc ctataatatt aggccttctt ttgtgaaaag gagtatattt
     1681 atataactga ataaaaatat atattgtgac aaatgcccat gcaaaaaatc gtgaaaaaag
     1741 gaagaaagtt ctactattct atgtaaaaat aaacccatta gatttttttc ctctttgtgt
     1801 aatgctcact accgcgatta tcgttcgttt catctggaca tgccaacgtt cttgaccttt
     1861 tagaacttac gaccggatga atactgtcat catcacatgc aagtaagttt ttggtcataa
     1921 agtcgacgat ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1981 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2041 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2101 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2161 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     2221 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     2281 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3601 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4081 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4141 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4201 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
