LOCUS       40924_25571        8008 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pINT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2007) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   4  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   5  (bases 1 to 8008)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 8008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-MAY-2007) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2009) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Jul 10, 2009 this sequence version replaced DQ813654.2.
FEATURES             Location/Qualifiers
     source          1..8008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(40..876)
                     /label=derived from hsp86 3' UTR
                     /note="derived from hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(885..1679)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    complement(1699..2544)
                     /label=contains promoter and 5' UTR from hsp86 gene
                     /note="contains promoter and 5' UTR from hsp86 gene"
     misc_feature    2552..3144
                     /label=contains promoter and 5' UTR from PcDT gene
                     /note="contains promoter and 5' UTR from PcDT gene"
     CDS             4679..4708
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      4718..5308
                     /label=derived from hrp2 3' UTR
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     promoter        complement(5326..5343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5350..5366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5840..5944
                     /label=AmpR promoter
     CDS             5945..6802
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6976..7564
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7852..7873
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7888..7918
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7926..7942
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7950..7966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7984..8002
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgggg ggatcctcta gagtcgacct gcaggcatgc tatttgatga attaactaca
       61 cttaaaataa tacaattatt attaaatttt tttttgattt atttattaat ttttaaactt
      121 aatcatttgt atttgggagg aattatatat atctttataa ttattttatt tttttttatt
      181 tttttatttt tttattatta ttattttttt ttattttttt tttttactgt atcaaagaaa
      241 aacctttaaa aaaaaaatta taatttcccc atcttactat atttttaata catacgtttt
      301 aaggaattaa attagacaaa agctatatta tgctttacat ataattagaa tttataaacg
      361 tttggttatt agatatttca tgtctcagta aagtctttca atacatatgt aaaaaaatat
      421 atatgaatac acataagttg ttaatatatt ttatatgcat aaatgtataa atatatatat
      481 atatatatat atatgtatgt atgtatatgt gtgtatatga aattatttca atgtttaatt
      541 ttttaaattt taattttttt tttttttttt ttttttatta tgtatattga tctttattat
      601 ttaaatatta cttttttcgt tttttcttct ttttattatt tttttttttt tttatatttt
      661 atacaaatgg taattcaaat aaaaggtata aatttatatt taattttctt ttatggataa
      721 ataaaagaaa aatataaata tataaaaata taaaaatata tatatgtata ttggggtgat
      781 gataaaatga aagataatat atatatatat atatctttat tttttttttt ttgtagaccc
      841 cattgtgagt acataaatat attatataac tcgaccttaa gtcagaagaa ctcgtcaaga
      901 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
      961 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     1021 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     1081 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg
     1141 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     1201 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     1261 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     1321 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     1381 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     1441 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     1501 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     1561 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     1621 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgac
     1681 attttgttat cacctaggtt tattcgaaat gtgggaagaa aaaaaatata ataaaaaagg
     1741 aaaatttctt tttaaagtaa aatatttatg taaatattta tatattttat tatatatata
     1801 ttattatata tatatatata tatatatata taccaataaa ataatataaa aatttttatg
     1861 tatatctaaa aatatgtttt tattaaaagg taaaaataaa cttatttttt cttttttttt
     1921 aaaaaatata tatatatata tatatatata atattatatg agatatatat taaatattat
     1981 attatttata taaatataat ttttttctta atttctcacg ttgttaaaat ttatatattt
     2041 atatatgata tatatttttt aatacttttt aatttttttt attatttttg gtaattatct
     2101 tattttattt taattttatt attttatatt aaatgatgct ttttatttta tattattatt
     2161 ttttatttaa aatttttgct aaacatgaaa ttattttatt atattttgta aatacaaaat
     2221 aaaattatat ataaatcaaa aaaaaaaaaa aagaaagaaa gaaagaaaga tgatatttaa
     2281 aaaaaaaaag atattataaa aatataatat tatatataaa atattatttt tatataaata
     2341 tatttatata tttatatata tgtatatata tatatatata tatataggta tagtatttat
     2401 tattttgtta tatttatata atactccttt ttgttatttt tttttttttt ttctttaaat
     2461 tcatgcaaaa atttactata atattattat atttaactat atactatgga atactaaata
     2521 tatatccaat ggcccctttg ggcccgcatg cttagctaat tcgcttgtaa gaggtactct
     2581 cgtttatgca aaactatttg atatagcatt ttaacaagta cacatatata tatgtaatat
     2641 atatactata tatatctatt gcatgtgtac taagcatgtg catggcatcc cctttttctc
     2701 gtgtttaaaa cagtttgtat gataaaatat aaaggatttg aaaaagagaa aaaaatatat
     2761 gatctcatcc tatatagcgc cataattttt atttgggttg aataaaattt tctactaaat
     2821 ttaggtgtaa gtaaaataat ggaatatata taagtacaat aaaaaagtgc ataaattaaa
     2881 aaatttttat aataaatatt ttttttaaaa aagtcaataa taatattaaa tatatataac
     2941 acaggattat atatgttcac tacaattttt tatattataa tataaattct tttcaatttt
     3001 cattttattt tacatacact ttcctttttt gtcactatat tttaatattc acatatttag
     3061 tttaaatact ggctatttct ttctacattt gctagtaaca attgtgtagt gcttatatat
     3121 atacacacac ctaaaactta caaaccatgg tgataacaaa atgagagccc tggtagtcat
     3181 ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg agtcttgcca
     3241 gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc tggacgtctc
     3301 cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt ggctagcgtt
     3361 cgaggagcaa ccgttcgacg tgatcgtggc gtaccgggta gaccggttga cccgatcgat
     3421 ccggcatctg cagcagctgg tccactgggc cgaggaccac aagaagctgg tcgtctccgc
     3481 gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg
     3541 aacggtggcg cagatggaat tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt
     3601 caatatccgc gccgggaaat accgaggatc cctgccgccg tggggatacc tgcctacgcg
     3661 cgtggacggg gagtggcggc tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt
     3721 gtatcaccgc gtcgtcgaca accacgagcc gctgcacctg gtggcccacg acctgaaccg
     3781 gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg caaggccgcg agccgcaggg
     3841 ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc tccgaggcga tgctcgggta
     3901 cgcgactctg aacggtaaga ccgtccgaga cgacgacgga gccccgctgg tgcgggctga
     3961 gccgatcctg acccgtgagc agctggaggc gctgcgcgcc gagctcgtga agacctcccg
     4021 ggcgaagccc gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg
     4081 cggggagccc gcgtacaagt tcgccggggg aggacgtaag cacccgcgct accgctgccg
     4141 ctcgatgggg ttcccgaagc actgcgggaa cggcacggtg gcgatggccg agtgggacgc
     4201 gttctgcgag gagcaggtgc tggatctgct cggggacgcg gagcgtctgg agaaagtctg
     4261 ggtagccggc tcggactccg cggtcgaact cgcggaggtg aacgcggagc tggtggacct
     4321 gacgtcgctg atcggctccc cggcctaccg ggccggctct ccgcagcgag aagcactgga
     4381 tgcccgtatt gcggcgctgg ccgcgcggca agaggagctg gagggtctag aggctcgccc
     4441 gtctggctgg gagtggcgcg agaccgggca gcggttcggg gactggtggc gggagcagga
     4501 caccgcggca aagaacacct ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg
     4561 cggcgggctg actcgcacga tcgacttcgg ggatctgcag gagtacgagc agcatctcag
     4621 gctcggcagc gtggtcgaac ggctacacac cgggatgtcg agagcggccg cactcgagga
     4681 acaaaagcta ataagtgaag aagatttata aggtacccca ttaaatttat ttaataatag
     4741 attaaaaata ttataaaaat aaaaacataa acacagaaat tacaaaaaaa atacatatga
     4801 attttttttt tgtaatcttc cttataaata tagaataatg aatcatataa aacatatcat
     4861 ttttcattta tttacattta aaattattgt ttcagtatct ttaatttatt atgtatatat
     4921 aaaaataact tacaatttta ttaataaaca atatatgttt attaattcat gttttgtaat
     4981 ttatgggata gcgatttttt ttactgtctg tatttttctt ttttaattat gttttaattg
     5041 tattttattt ttattattgt tctttttata gtattatttt aaaacaaaat gttttttcta
     5101 agaacttata ataataataa atataaattt taataaaaat tatatttatc ttttacaata
     5161 tgaacataaa gtacaacatt aatatatagc ttttaatatt tttattccta atcatgtaaa
     5221 tcttaaattt ttctttttaa acatatgtta aatatttatt tctcattata tataaggaca
     5281 tatttattaa accgcagaga aatctagagg taccgagctc gaattctata gtgagtcgta
     5341 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5401 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5461 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     5521 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     5581 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5641 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5701 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5761 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5821 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5881 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5941 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6001 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6061 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6121 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6181 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6241 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6301 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6361 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6421 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6481 gatgcctgta gcaatgccaa caacgttgcg caaactatta actggcgaac tacttactct
     6541 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6601 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6661 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6721 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6781 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6841 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6901 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7081 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatc
     7561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7621 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7681 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7741 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7801 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7861 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7921 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7981 gctatttagg tgacactata gaatactc
//
