LOCUS       62056_12890        4421 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     polyA_signal    complement(1586..1707)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1811..2205)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2222..2593)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(3186..3489)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3569..4157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4265..4313
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4324..4415
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgtcgacgag ctcgatatcg gccatggtta
      661 cgcgtctcga gagatctgtg gagtgcccac cgtgcccagg taagccagcc caggcctcgc
      721 cctccagctc aaggcgggac aggtgcccta gagtagcctg catccaggga caggccccag
      781 ctgggtgctg acacgtccac ctccatctct tcctcagcac cacctgtggc aggaccgtca
      841 gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc
      901 acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg
      961 gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg
     1021 ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac
     1081 aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc
     1141 aaaggtggga cccgcggggt atgagggcca catggacaga ggccggctcg gcccaccctc
     1201 tgccctggga gtgaccgctg tgccaacctc tgtccctaca gggcagcccc gagaaccaca
     1261 ggtgtacacc ctgcccccat cccgggagga gatgaccaag aaccaggtca gcctgacctg
     1321 cctggtcaaa ggcttctacc ccagcgacat cgccgtggag tgggagagca atgggcagcc
     1381 ggagaacaac tacaagacca cacctcccat gctggactcc gacggctcct tcttcctcta
     1441 cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt
     1501 gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa
     1561 atgagtgcta gctggccaga catgataaga tacattgatg agtttggaca aaccacaact
     1621 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     1681 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1741 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1801 gaattaattc taaaatacag catagcaaaa ctttaacctc caaatcaagc ctctacttga
     1861 atccttttct gagggatgaa taaggcatag gcatcagggg ctgttgccaa tgtgcattag
     1921 ctgtttgcag cctcaccttc tttcatggag tttaagatat agtgtatttt cccaaggttt
     1981 gaactagctc ttcatttctt tatgttttaa atgcactgac ctcccacatt ccctttttag
     2041 taaaatattc agaaataatt taaatacatc attgcaatga aaataaatgt tttttattag
     2101 gcagaatcca gatgctcaag gcccttcata atatccccca gtttagtagt tggacttagg
     2161 gaacaaagga acctttaata gaaattggac agcaagaaag cgagcttcta gcttatcctc
     2221 agtcctgctc ctctgccaca aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     2281 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     2341 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     2401 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     2461 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     2521 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     2581 tcaacttggc catgatggct cctcctgtca ggagaggaaa gagaagaagg ttagtacaat
     2641 tgctatagtg agttgtatta tactatgcag atatactatg ccaatgatta attgtcaaac
     2701 tagggctgca gggttcatag tgccactttt cctgcactgc cccatctcct gcccaccctt
     2761 tcccaggcat agacagtcag tgacttacca aactcacagg agggagaagg cagaagcttg
     2821 agacagaccc gcgggaccgc cgaactgcga ggggacgtgg ctagggcggc ttcttttatg
     2881 gtgcgccggc cctcggaggc agggcgctcg gggaggccta gcggccaatc tgcggtggca
     2941 ggaggcgggg ccgaaggccg tgcctgacca atccggagca cataggagtc tcagcccccc
     3001 gccccaaagc aaggggaagt cacgcgcctg tagcgccagc gtgttgtgaa atgggggctt
     3061 gggggggttg gggccctgac tagtcaaaac aaactcccat tgacgtcaat ggggtggaga
     3121 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     3181 atcatcatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     3241 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     3301 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     3361 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     3421 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     3481 tatgtaacgc ctgcaggtta attaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tggctagtta attaacattt aaatcagcgg
     4261 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgta
     4321 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     4381 ccccagtgca agtgcaggtg ccagaacatt tctctatcga a
//
