LOCUS       V013038                 4806 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013038
VERSION     V013038
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..162)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        636..740
                     /label="AmpR promoter"
     CDS             741..1598
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1772..2360
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2648..2669
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2684..2714
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2722..2738
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2746..2762
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3130..3861
                     /gene="ermC'"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Bacillus
                     subtilis. Accession#: P13956"
ORIGIN
        1 agcttggctg cagtataaaa tataaataat tttctaaaaa acttaacttc atgtgaaaag
       61 tttgttaaaa tataaatgag cacgttaatc atttaacata gataattaaa tagtaaaagg
      121 gagtgtcgag gatccccggg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      301 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
      361 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      421 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      481 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      601 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      781 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      841 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      901 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      961 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1021 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1081 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1141 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1201 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1261 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1321 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1381 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1441 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1501 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1561 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1621 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1681 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1741 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1801 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1861 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1921 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1981 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2041 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2101 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2161 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2221 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2281 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2341 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2401 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2461 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2521 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2581 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2641 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2701 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2761 accatgatta cgccaagctt tggctaacac acacgccatt ccaaccaata gttttctcgg
     2821 cataaagcca tgctctgacg cttaaatgca ctaatgcctt aaaaaaacat taaagtctaa
     2881 cacactagac ttatttactt cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaag
     2941 tataaaacct ttaagaactt tcttttttct tgtaaaaaaa gaaactagat aaatctctca
     3001 tatcttttat tcaataatcg catcagattg cagtataaat ttaacgatca ctcatcatgt
     3061 tcatatttat cagagctcgt gctataatta tactaatttt ataaggagga aaaaataaag
     3121 agggttataa tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat
     3181 aatatagata aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc
     3241 ggctcaggaa aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc
     3301 attgaaatag accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat
     3361 ttccaagttt taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa
     3421 atatttggta atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat
     3481 agtatagctg atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat
     3541 acaaaacgct cattggcatt atttttaatg gcagaagttg atatttctat attaagtatg
     3601 gttccaagag aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat
     3661 agaaaaaaat caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa
     3721 tgggttaaca aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa
     3781 catgcaggaa ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat
     3841 agctataaat tatttaataa gtaagttaag ggatgcataa actgcatccc ttaacttgtt
     3901 tttcgtgtac ctattttttg tgaatcgatt atgtcttttg cgcattcact tcttttctat
     3961 ataaatatga gcgaagcgaa taagcgtcgg aaaagcagca aaaagtttcc tttttgctgt
     4021 tggagcatgg gggttcaggg ggtgcagtat ctgacgtcaa tgccgagcga aagcgagccg
     4081 aagggtagca tttacgttag ataaccccct gatatgctcc gacgctttat atagaaaaga
     4141 agattcaact aggtaaaatc ttaatatagg ttgagatgat aaggtttata aggaatttgt
     4201 ttgttctaat ttttcactca ttttgttcta atttctttta acaaatgttc tttttttttt
     4261 agaacagtta tgatatagtt agaatagttt aaaataagga gtgagaaaaa gatgaaagaa
     4321 agatatggaa cagtctataa aggctctcag aggctcatag acgaagaaag tggagaagtc
     4381 atagaggtag acaagttata ccgtaaacaa acgtctggta acttcgtaaa ggcatatata
     4441 gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa aacttaaaat cgttaactat
     4501 atcctagata atgtccactt aagtaacaat acaatgatag ctacaacaag agaaatagca
     4561 aaagctacag gaacaagtct acaaacagta ataacaacac ttaaaatctt agaagaagga
     4621 aatattataa aaagaaaaac tggagtatta atgttaaacc ctgaactact aatgagaggc
     4681 gacgaccaaa aacaaaaata cctcttactc gaatttggga actttgagca agaggcaaat
     4741 gaaatagatt gacctcccaa taacaccacg tagttattgg gaggtcaatc tatgaaaatg
     4801 cgatta
//
