LOCUS       40924_25466        9901 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible expression vector pILL2157, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Boneca IG, Ecobichon C, Chaput C, Mathieu A, Guadagnini S, Prevost 
            MC, Colland F, Labigne A, de Reuse H.
  TITLE     Development of inducible systems to engineer conditional mutants of 
            essential genes of Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 2095-2102 (2008)
  PUBMED    18245237
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Boneca IG, Ecobichon C, de Reuse H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2008) Microbiology, Institut Pasteur, 28 rue du 
            Dr. Roux, Paris 75724, France
REFERENCE   3  (bases 1 to 9901)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "7"; pages:
            "2095-2102"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2008) Microbiology, Institut Pasteur, 28 rue du Dr. Roux, 
            Paris 75724, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(168..189)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(205..1284)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(1285..1536)
                     /label=amiE promoter region
                     /note="amiE promoter region"
                     /regulatory_class="promoter"
     CDS             4546..5202
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      5334..5382
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(5425..8493)
                     /label=lacZ
                     /note="beta-galactosidase"
     protein_bind    8525..8541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(8546..8562)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(8922..9510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            9736..9845
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 agaagcttaa cattatccag aacgggagtg cgccttgagc gacacgaatt atgcagtgat
       61 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
      121 gcaccgtgca gtcgatgata agctgtcaaa catgagaatt gtgcctaatg agtgagctaa
      181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
      301 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
      361 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
      421 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
      481 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
      541 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
      601 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
      661 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
      721 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
      781 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
      841 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
      901 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
      961 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     1021 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     1081 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     1141 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     1201 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     1261 atcgtataac gttactggtt tcactattcc ctccttaatt aattcctaca aaccctctac
     1321 ttgaatttat aaaataattg tgtaaacgga tttataatga atatgaaaac tttaatcatt
     1381 atgattattg tgggcgagtt ttaacttttt tgctccattg ggtaacctat ttctaaaaat
     1441 gaggttttag ccgctctgtt tttatagtta ttttattttc acttccacta agccatatag
     1501 cgcgttaata caatagattt tagacgcttt ttgcaagtcc gttagcaaat ataatgtgtt
     1561 tagcacctaa aagcgcatct atcttgtgtt agccatctaa cctaaacaaa accgcctagc
     1621 gagcgttagc gagcatggac aaaagcgcat cgcagtttga aatcgtaggc gttagcagga
     1681 tggggttttg cgttagcaaa atcaaacaag ntagcggaaa accggggggg tcaccaaaaa
     1741 aaaccctaaa actaaaatcc ccaaaatatg tagcgcgtca tgcgcgttgt ttttattaca
     1801 tgttttaata actatgttgt ttttacatgt ttttacaacg cgcgcgcgtg tgagggattg
     1861 ggggttgcaa ccccctaaat aacgaagctg tagggtttct catttttggg ttaaaatgaa
     1921 taaaacagaa cttcttacga gtgataacag aacttcttac gagtgataac agaacttctt
     1981 acgagtgata acagaacttc ttacgagtga taacagaact tctttattgt gaagttatga
     2041 ttattaacaa tttttagcta aaataacaac ctaaggcggt gtaacatggt tgctaatcct
     2101 agtgttaaca aatttggagc aattagcttt aaaagctagt gggttgggag tttgtagcgg
     2161 gtatgcactc cgttaggagg cacaccatga aagcattttt gatagtagtg attttagtgg
     2221 taatcttgac acagccacta tattaaaacc ttagcgtttt aataaccctt ataagtccgc
     2281 caagacttct taagggtttc actcctgtta ttatatcgtc ttttgaaaaa taagcattaa
     2341 aaggcgctta aatgcccatg aatacgaatt ttgaacagct tagaaaacaa gaattggaat
     2401 tacgaaaatt attagaagaa ttagaaacgc tcccacaaac cccacaaatt aaactgcaaa
     2461 aacaaaaaat acaaacttac atagacaaga taacaccaag tattttgagc ggttttgatc
     2521 aaaaattcaa agaaattata gaaaatctat caaatgaatt tgaaaaagaa aaatccacac
     2581 cactcaaaga gccacaaacc acccccacac catgcaaaga tttagtggtt agcaccccta
     2641 aagataacac ctataccacc taccacaata acgctaataa ggtcaatcta gggaaattga
     2701 gcgaaaggga agccaatctt ttattcgcta tttttcaaaa actcaaagat caagggaata
     2761 ccctcattcg ttttgaaccg caagatttga aacgcatgat catggtcaaa tccaacctaa
     2821 ccaacagaca attattgcaa atcctcaaaa acttacttga caacataggc ggtgctaatt
     2881 tttggatcat tagagagcat gttgaaaatg gcgaaatcta tgaagatcac actagctaca
     2941 tgcttttcaa acaatttgac attcgtatcc ataagccaac ccaaactata gaatacttag
     3001 aagtccaact caacgatagc taccactacc tgctcaacaa tctaggcatg ggcggtcaat
     3061 acacttcctt taaactttta gaattccaaa gggtgagggg caaatacgcc aagacgctct
     3121 atcgcttact caagcaatac aaaagcacag ggattttgag cgtggaatgg gatcaattca
     3181 gggagctttt agacattcca aaagattaca aaatggaaaa catcgatcaa aaagtcttaa
     3241 ctccaagcct caaggaactc agaaaaatct atccctttga acacttgagc tacaaaaaag
     3301 aacgcaaaag ccacgacaaa cgcaaggtaa cgcatattga tttttatttt gagcaattgc
     3361 cacagggcga aaccaagaaa caaaaacaag ccgacaagca acgcgctcaa agggacatca
     3421 agctcatagc gtgggatatt cacaaccaaa tcgctaaaag aaacgctaaa gccactatag
     3481 aagctaggtt tcttgagcta aaaaccttga ttggttatca gttcaggcac aacaatggga
     3541 ctattttaca gattgacaac gccacttttg aaaagaatca aatgtttttg catgtttcaa
     3601 ccagcaaaaa ttctcaaaaa ttccttgtgt ccaacaaaac attcgcttta gaacttctgt
     3661 ttgtcaatgg atacagccta aaaaaagaca tgttggaaat tgatccccca agtatccacc
     3721 ccatcaccaa cgaacctatc aaggaatttg cagaatacat cggaaaaacg atccacatca
     3781 ccaatttcaa tgtggatcaa tgccctgagg ggatcaataa ctacctgaaa atcactagga
     3841 ttgtgaaact gaatgacaat cggatctgtg tttcagtcca agatgtggat aagcctgaga
     3901 aacttctaaa acctttcatt gctaaagatg aaaaacattt gaaaaattgg tttaagaaac
     3961 actatcggtg aaaaataagt ggtgggatta acaatcgtcc cccacacctt gcaatgtagt
     4021 tagttttgca ttattttttc tataatctct atcaattctg agagaacata gattaattca
     4081 atgaaatttt tcattatcta tttcctttct gtaaattttg ttaaatattt attaaaaaaa
     4141 aatttaaaga tcactcttta agtggaacat acaaaggctg tagagagtgc cacattaagc
     4201 ctgatgtttt gcttgtgtat agagtgaaag gcaatatttt aactttagtt aggcttggca
     4261 gtcatagtga attgttttag acaaatttct ttctaaaatc gcccagaatg aatttttaat
     4321 tctaatttaa tataattcat cggttttcac tcaaaacgat gaaatagggc tatttttagc
     4381 tcattattgg ggtattttgt ttttagttga aagctaactt caaaattcaa aaaaacttca
     4441 tttccctttc tagagatccg ccatattgtg ttgaaacacc gcccggaacc cgatataatc
     4501 cgcccttcaa cagatccgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     4561 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     4621 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     4681 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     4741 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     4801 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     4861 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     4921 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     4981 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     5041 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     5101 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     5161 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     5221 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     5281 ggcagaaatt cggatcttcc atacctacca gttctgcgcc tgcaggtcga taaaccgata
     5341 caattaaagg ctccttttgg agcctttttt tttggagatt ttcaacgtgg atctgaattc
     5401 gagctcggta cccggggatc cttatttttg acaccagacc aactggtaat ggtagcgacc
     5461 ggcgctcagc tggaattccg ccgatactga cgggctccag gagtcgtcgc caccaatccc
     5521 catatggaaa ccgtcgatat tcagccatgt gccttcttcc gcgtgcagca gatggcgatg
     5581 gctggtttcc atcagttgct gttgactgta gcggctgatg ttgaactgga agtcgccgcg
     5641 ccactggtgt gggccataat tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg
     5701 gaagacgtac ggggtataca tgtctgacaa tggcagatcc cagcggtcaa aacaggcggc
     5761 agtaaggcgg tcgggatagt tttcttgcgg ccctaatccg agccagttta cccgctctgc
     5821 tacctgcgcc agctggcagt tcaggccaat ccgcgccgga tgcggtgtat cgctcgccac
     5881 ttcaacatca acggtaatcg ccatttgacc actaccatca atccggtagg ttttccggct
     5941 gataaataag gttttcccct gatgctgcca cgcgtgagcg gtcgtaatca gcaccgcatc
     6001 agcaagtgta tctgccgtgc actgcaacaa cgctgcttcg gcctggtaat ggcccgccgc
     6061 cttccagcgt tcgacccagg cgttagggtc aatgcgggtc gcttcactta cgccaatgtc
     6121 gttatccagc ggtgcacggg tgaactgatc gcgcagcggc gtcagcagtt gttttttatc
     6181 gccaatccac atctgtgaaa gaaagcctga ctggcggtta aattgccaac gcttattacc
     6241 cagctcgatg caaaaatcca tttcgctggt ggtcagatgc gggatggcgt gggacgcggc
     6301 ggggagcgtc acactgaggt tttccgccag acgccactgc tgccaggcgc tgatgtgccc
     6361 ggcttctgac catgcggtcg cgttcggttg cactacgcgt actgtgagcc agagttgccc
     6421 ggcgctctcc ggctgcggta gttcaggcag ttcaatcaac tgtttacctt gtggagcgac
     6481 atccagaggc acttcaccgc ttgccagcgg cttaccatcc agcgccacca tccagtgcag
     6541 gagctcgtta tcgctatgac ggaacaggta ttcgctggtc acttcgatgg tttgcccgga
     6601 taaacggaac tggaaaaact gctgctggtg ttttgcttcc gtcagcgctg gatgcggcgt
     6661 gcggtcggca aagaccagac cgttcataca gaactggcga tcgttcggcg tatcgccaaa
     6721 atcaccgccg taagccgacc acgggttgcc gttttcatca tatttaatca gcgactgatc
     6781 cacccagtcc cagacgaagc cgccctgtaa acggggatac tgacgaaacg cctgccagta
     6841 tttagcgaaa ccgccaagac tgttacccat cgcgtgggcg tattcgcaaa ggatcagcgg
     6901 gcgcgtctct ccaggtagcg aaagccattt tttgatggac catttcggca cagccgggaa
     6961 gggctggtct tcatccacgc gcgcgtacat cgggcaaata atatcggtgg ccgtggtgtc
     7021 ggctccgccg ccttcatact gcaccgggcg ggaaggatcg acagatttga tccagcgata
     7081 cagcgcgtcg tgattagcgc cgtggcctga ttcattcccc agcgaccaga tgatcacact
     7141 cgggtgatta cgatcgcgct gcaccattcg cgttacgcgt tcgctcatcg ccggtagcca
     7201 gcgcggatca tcggtcagac gattcattgg caccatgccg tgggtttcaa tattggcttc
     7261 atccaccaca tacaggccgt agcggtcgca cagcgtgtac cacagcggat ggttcggata
     7321 atgcgaacag cgcacggcgt taaagttgtt ctgcttcatc agcaggatat cctgcaccat
     7381 cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt taacgcctcg
     7441 aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca cctcgcggaa
     7501 accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac
     7561 cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga cgttcagacg
     7621 tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc
     7681 ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc gtaggtagtc
     7741 acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga aatcatcatt
     7801 aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca gcaacgagac
     7861 gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac tgccgtcact
     7921 ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc
     7981 aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag
     8041 atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct gtagccagct
     8101 ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg gaacaaacgg
     8161 cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt aaccgtgcat
     8221 ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact ccagccagct
     8281 ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg
     8341 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8401 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     8461 taaaacgacg gccagtgaat ccgtaatcat ggtactagtc atatgctttt ccttccaaac
     8521 aaaattgtta tccgctcaca attaattgtt atccgctcac aattaaattt ttacaacaaa
     8581 cgcaccttac gaataacttt tgttgcttga gctaatcata aagaaaaata gtaaattgac
     8641 aaaaaataaa aacaaaaaaa gcccccaatt ttttgataaa caaaaaatca gagagctaaa
     8701 aactaaggat ttaaggagcg ttgctcctag gaagatctag atatcgatga attcaaaaaa
     8761 aaaaggctcc aaaaggagcc tttaatgatc taattcgtat cagctcactc aaaggcggta
     8821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9241 ccggtaagac acgacttatc gccaccggca gcagccactg gtaacaggat tagcagagcg
     9301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9361 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatctctgc agttcagtaa
     9541 tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg
     9601 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     9661 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     9721 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     9781 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     9841 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagctgca
     9901 g
//
