LOCUS       62056_12760        9008 bp DNA     circular SYN 11-AUG-2020
DEFINITION  Cloning vector pIG6-Sia, complete sequence.
ACCESSION   MK353499
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Hu X, Ruan Y, Ding N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2019) Medical College, Dalian University, Xuefu 
            road 10, dalian 116600, China
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-JAN-2019) Medical College, Dalian University, Xuefu road 10, 
            dalian 116600, China"
FEATURES             Location/Qualifiers
     source          1..9008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..82
                     /label=lacI promoter
     CDS             83..1162
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1178..1199
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1214..1244
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1252..1268
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1276..1292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1312..1327
                     /label=ompA RBS
                     /note="ompA RBS"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     1328..1390
                     /label=OmpA signal peptide
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             1736..1753
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1828..1875
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      1901..2232
                     /note="regulatory region promoter from from pgl locus in 
                     Campylobacter jejuni"
                     /regulatory_class="promoter"
     gene            2236..3780
                     /gene="Delta16psp2,6ST"
                     /label=Delta16psp2,6ST
     CDS             2236..3780
                     /codon_start=1
                     /transl_table=11
                     /gene="Delta16psp2,6ST"
                     /product="protein Delta16psp2,6ST"
                     /label=Delta16psp2,6ST
                     /note="optimized 2,6-sialyltransferase from Vibrionaceae 
                     Photobacterium sp. JT-ISH-224"
                     /protein_id="QEA03482.1"
                     /translation="MKNFLLLTLILLTACNNSEENTQSIIKNDINKTIIDEEYVNLEPI
                     NQSNISFTKHSWVQTCGTQQLLTEQNKESISLSVVAPRLDDDEKYCFDFNGVSNKGEKY
                     ITKVTLNVVAPSLEVYVDHASLPTLQQLMDIIKSEEENPTAQRYIAWGRIVPTDEQMKE
                     LNITSFALINNHTPADLVQEIVKQAQTKHRLNVKLSSNTAHSFDNLVPILKELNSFNNV
                     TVTNIDLYDDGSAEYVNLYNWRDTLNKTDNLKIGKDYLEDVINGINEDTSNTGTSSVYN
                     WQKLYPANYHFLRKDYLTLEPSLHELRDYIGDSLKQMQWDGFKKFNSKQQELFLSIVNF
                     DKQKLQNEYNSSNLPNFVFTGTTVWAGNHEREYYAKQQINVINNAINESSPHYLGNSYD
                     LFFKGHPGGGIINTLIMQNYPSMVDIPSKISFEVLMMTDMLPDAVAGIASSLYFTIPAE
                     KIKFIVFTSTETITDRETALRSPLVQVMIKLGIVKEENVLFWADLPNCETGVCIAV"
     regulatory      3781..3796
                     /label=ompA RBS
                     /note="ompA RBS"
                     /regulatory_class="ribosome_binding_site"
     gene            3797..4837
                     /gene="nueB"
                     /label=nueB
     CDS             3797..4837
                     /codon_start=1
                     /transl_table=11
                     /gene="nueB"
                     /product="NueB"
                     /label=nueB
                     /note="sialic acid synthase from Campylobacter jejuni"
                     /protein_id="QEA03485.1"
                     /translation="MKEIKIQNIIISEEKAPLVVPEIGINHNGSLELAKIMVDAAFSAG
                     AKIIKHQTHIVEDEMSKAAKKVIPGNAKISIYEIMQKCALDYKDELALKEYTEKLGLVY
                     LSTPFSRAGANRLEDMGVSAFKIGSGECNNYPLIKHIAAFKKPMIVSTGMNSIESIKPT
                     VKILLDNEIPFVLMHTTNLYPTPHNLVRLNAMLELKKEFSCMVGLSDHTTDNLACLGAV
                     VLGACVLERHFTDSMHRSGPDIVCSMDTKALKELIIQSEQMAIIRGNNESKKAAKQEQV
                     TIDFAFASVVSIKDIKKGEVLSMDNIWVKRPGLGGISAAEFENILGKKALRDIENDAQL
                     SYEDFA"
     gene            4834..5952
                     /gene="neuC"
                     /label=neuC
     CDS             4834..5952
                     /codon_start=1
                     /transl_table=11
                     /gene="neuC"
                     /product="NeuC"
                     /label=neuC
                     /note="N-acetylglucosamine-6-phosphate 2-epimerase from 
                     Campylobacter jejuni"
                     /protein_id="QEA03484.1"
                     /translation="MKKILFITGSRADYSKIKSLMYRVQNSSEFELYIFATGMHLSKNF
                     GYTVKELYKNGFKNIYEFINYDKYYQTDKALATTIDGFSRYANELKPDLIVVHGDRIEP
                     LAAAIVGALNNILVAHIEGGEISGTIDDSLRHAISKLAHIHLVNDEFAKRRLMQLGEDE
                     KSIFIIGSPDLELLNDNKISLSEAKKYYDINYENYALLMFHPVTTEITSIKNQADNLVK
                     ALIQSNKNYIVIYPNNDLGFELILQSYEEFKNNPRFKLFPSLRFEYFITLLKNADFIIG
                     NSSCILKEALYLKTAGILVGSRQNGRLGNENTLKVNANSDEILKAINTIHKKQDLFSAK
                     LEILDSSKLFFEYLQSGDFFKLSTQKVFKDIK"
     gene            5949..6614
                     /gene="neuA"
                     /label=neuA
     CDS             5949..6614
                     /codon_start=1
                     /transl_table=11
                     /gene="neuA"
                     /product="NeuA"
                     /label=neuA
                     /note="CMP-Neu5Ac synthetase from Campylobacter jejuni"
                     /protein_id="QEA03483.1"
                     /translation="MSLAIIPARGGSKGIKNKNLVLLNNKPLIYYTIKAALNAKSISKV
                     VVSSDSDEILNYAKSQNVDILKRPISLAQDDTTSDKVLLHALKFYKDYEDVVFLQPTSP
                     LRTNIHINEAFNLYKNSNANALISVSECDNKILKAFVCNDCGDLAGICNDEYPFMPRQK
                     LPKTYMSNGAIYILKIKEFLNNPSFLQSKTKHFLMDESSSLDIDCLEDLKKVEQIWKK"
     CDS             6621..6638
                     /label=6xHis
                     /note="6xHis affinity tag"
     rep_origin      6742..7197
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7224..7328
                     /label=AmpR promoter
     CDS             7329..8186
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8360..8948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
       61 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1201 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1261 gataacaatt tcacacagga aacagctatg accatgatta cgaatttcta gataacgagg
     1321 gcaaaaaatg aaaaagacag ctatcgcgat tgcagtggca ctggctggtt tcgctaccgt
     1381 agcgcaggcc gactacaaag atatccgtga cctggaagtg gtcgctgcca caccgacgag
     1441 tctgctgatt tcttgggatg caccagctgt aaccgtgcgc tactaccgca ttacttacgg
     1501 ggagacgggc ggcaattccc cggtgcaaga atttactgtt ccgggcagca aaagtacagc
     1561 aactattagc ggcctgaaac cgggcgttga ttataccatt actgtttacg cagtaactgg
     1621 gcgtggcgat tcaccggcgt cctctaaacc tatttcgatc aactatcgta ctgaaatcgg
     1681 tggtggtggt tctgaccaaa acgcgaccaa gcttggtggt ggtggttcac ttgagcacca
     1741 ccaccaccac cactgagaat tcgagatccg gctgctaaca aagcccgaaa ggaagctgag
     1801 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1861 ttgaggggtt ttttgctgaa aggaggaact atatccggat gggtttacca ggaaaatatg
     1921 atgtgcatgg cgatgaggtg atgaaacttt tttgtgaaaa tgaacttgaa aaaattcacg
     1981 aatattgtga aagtgatgtt ttaaatactt atatgctttt tttaaaatat gaacttatta
     2041 aagctaatgt tagtgaagaa gattatattg attttctttc ttatatgaga gattttttgc
     2101 gtgcaaaaaa atcagatcgt tcctatacag aagtttttgc aaaagcttgt gagagtgaaa
     2161 tttcaaaagt tcaatcttaa gtatttaaga aaatatatta aaatttattt ttgatatttt
     2221 ttagaaaaag gacatatgaa gaacttcctg ctgctgaccc tgatcctgct gaccgcgtgc
     2281 aacaacagcg aggaaaacac ccagagcatc atcaagaacg atatcaacaa gaccatcatc
     2341 gacgaggaat acgtgaacct ggaaccgatc aaccaaagca acattagctt taccaaacac
     2401 agctgggttc agacctgcgg cacccagcaa ctgctgaccg aacaaaacaa ggagagcatc
     2461 agcctgagcg tggttgcgcc gcgtctggac gatgacgaaa aatactgctt cgactttaac
     2521 ggcgtgagca acaagggcga gaaatatatt accaaggtta ccctgaacgt ggttgcgccg
     2581 agcctggaag tgtacgttga tcacgcgagc ctgccgaccc tgcagcaact gatggacatc
     2641 attaagagcg aggaagagaa cccgaccgcg cagcgttata tcgcgtgggg tcgtattgtg
     2701 ccgaccgatg aacaaatgaa agagctgaac atcaccagct tcgcgctgat taacaaccac
     2761 accccggcgg acctggtgca ggagatcgtt aagcaggcgc aaaccaaaca ccgtctgaac
     2821 gtgaagctga gcagcaacac cgcgcacagc ttcgataacc tggttccgat cctgaaagaa
     2881 ctgaacagct ttaacaacgt gaccgttacc aacattgacc tgtacgatga cggcagcgcg
     2941 gagtacgtta acctgtataa ctggcgtgat accctgaaca agaccgacaa cctgaagatc
     3001 ggtaaagatt acctggaaga cgtgatcaac ggcattaacg aggataccag caacaccggt
     3061 accagcagcg tttataactg gcagaagctg tacccggcga actatcactt tctgcgtaaa
     3121 gactacctga ccctggaacc gagcctgcac gagctgcgtg attatatcgg cgacagcctg
     3181 aagcagatgc aatgggatgg tttcaagaaa tttaacagca aacagcaaga actgttcctg
     3241 agcattgtga actttgacaa gcagaaactg caaaacgagt ataacagcag caacctgccg
     3301 aacttcgtgt ttaccggcac caccgtttgg gcgggtaacc acgagcgtga gtactatgcg
     3361 aaacagcaaa tcaacgttat taacaacgcg atcaacgaaa gcagcccgca ctacctgggc
     3421 aacagctacg acctgttctt caagggtcac ccgggtggcg gtatcattaa caccctgatt
     3481 atgcagaact acccgagcat ggtggacatc ccgagcaaaa ttagcttcga agttctgatg
     3541 atgaccgata tgctgccgga cgcggtggcg ggtatcgcga gcagcctgta ttttaccatt
     3601 ccggcggaga agatcaaatt cattgttttt accagcaccg aaaccatcac cgatcgtgag
     3661 accgcgctgc gtagcccgct ggtgcaagtt atgatcaagc tgggcattgt gaaagaagag
     3721 aacgttctgt tctgggcgga cctgccgaac tgcgagaccg gtgtgtgcat cgcggtttaa
     3781 ataacgaggg caaaaaatga aagaaataaa aatacaaaat ataatcataa gtgaagaaaa
     3841 agcaccctta gtcgtacctg aaataggcat taatcataat ggcagtttag aactagctaa
     3901 aattatggta gatgcagcct ttagcgcagg tgctaagatt ataaagcatc aaactcatat
     3961 tgttgaagat gagatgagta aggccgctaa aaaagtaatt cctggtaatg caaaaataag
     4021 catttatgag attatgcaaa aatgtgcttt ggattataaa gatgagctag cacttaaaga
     4081 atacacagaa aaattaggtc ttgtttatct tagcacacct ttttctcgtg caggtgcgaa
     4141 ccgcttagaa gatatgggag ttagtgcttt taagattggt tcaggtgagt gtaataatta
     4201 tccgcttatt aaacacatag cagcctttaa aaagcctatg atagttagca caggaatgaa
     4261 tagtattgaa agtataaaac caactgtaaa aatcttatta gacaatgaaa ttccttttgt
     4321 tttaatgcac acgaccaatc tttacccaac cccgcataat cttgtaagat taaacgctat
     4381 gcttgagtta aaaaaagaat tttcttgtat ggtaggctta agcgaccaca caacagataa
     4441 tcttgcgtgt ttaggtgcag ttgtacttgg agcttgtgtg cttgaaagac attttactga
     4501 tagtatgcat agaagtggcc ctgatatagt ttgttctatg gatacaaagg ctttaaaaga
     4561 gctaattata caaagtgagc aaatggctat aataagagga aataatgaaa gtaaaaaagc
     4621 ggctaaacaa gaacaagtta caattgattt tgcctttgca agtgtagtta gcattaaaga
     4681 tattaaaaaa ggcgaagttt tatctatgga taatatttgg gttaaaagac ctggacttgg
     4741 tggaattagt gcggctgaat ttgaaaatat tttaggcaaa aaagcattaa gagatataga
     4801 aaatgatgct cagttaagct atgaggattt tgcatgaaaa aaatcctttt tataacaggc
     4861 tctagggctg attattctaa gattaaatct ttaatgtaca gggtgcaaaa ctcaagcgaa
     4921 tttgaacttt acatctttgc aacaggaatg cacttaagta aaaattttgg ctatacagtt
     4981 aaagaacttt ataaaaatgg ctttaaaaat atttatgaat ttataaatta tgataaatat
     5041 tatcaaactg ataaggcttt agctactaca attgatggat tttcaaggta tgcaaatgag
     5101 ctaaaacctg atttaatcgt agtacatgga gatagaattg agcctttagc agcagctatt
     5161 gttggagcat taaataatat cttagtagcg catattgaag gcggagagat ttcaggaact
     5221 attgacgata gcttacgcca cgctatatca aaactagctc atattcattt agtaaatgat
     5281 gagtttgcaa aaaggcgttt aatgcagctt ggagaagatg aaaaatctat ttttatcata
     5341 ggttcgcctg atttagaact tttaaacgat aataaaattt cacttagcga agcaaaaaaa
     5401 tattatgata taaattatga aaactacgct ttgcttatgt ttcatcctgt tacaactgaa
     5461 attactagca ttaaaaatca agcagacaat ttagtaaaag cactgataca aagtaataaa
     5521 aattatattg ttatttatcc aaataatgat ttaggttttg aattaatctt gcaaagctat
     5581 gaagagttta aaaataaccc tagatttaag ctttttccat cgcttagatt tgagtatttt
     5641 ataactttgt taaaaaatgc tgattttata ataggtaatt caagttgtat tttaaaagag
     5701 gccttatact taaaaacagc agggatttta gttggctcaa gacaaaatgg aagacttggc
     5761 aatgaaaata cactaaaagt taatgcaaat agtgatgaaa tactaaaagc tattaacact
     5821 attcataaaa aacaagattt atttagcgct aagttagaga ttttagatag ctcaaaatta
     5881 ttttttgaat atttacaaag cggagatttt tttaaactca gcacacaaaa agtttttaag
     5941 gatataaaat gagcttagca ataatccctg ctcgtggtgg ctcaaagggt attaaaaata
     6001 aaaatttggt tttattaaac aataaacctt taatttacta cacgatcaaa gctgcactaa
     6061 atgctaaaag cattagtaaa gttgttgtaa gcagtgatag tgatgaaatt ttaaattatg
     6121 caaaaagtca aaatgttgat attttaaaac gcccaattag ccttgcacaa gatgatacca
     6181 caagcgataa agtgctgtta catgctctaa aattttataa agattatgaa gatgtagttt
     6241 ttttacaacc cacttcaccg ctaagaacaa atattcatat taatgaagct tttaatcttt
     6301 ataaaaatag caatgcaaat gccctaatta gcgtaagcga atgtgataat aaaattctaa
     6361 aagcctttgt ttgtaatgat tgtggcgatt tagcagggat ttgtaatgat gaatatcctt
     6421 ttatgccaag gcaaaaattg cctaaaactt atatgagcaa tggtgcaatt tatattttaa
     6481 agataaaaga atttttaaac aatcctagct ttttacaaag caaaaccaag cattttttaa
     6541 tggacgaaag ctcaagttta gatattgact gtttggagga tttaaaaaag gttgaacaga
     6601 tatggaaaaa ataactcgag caccaccacc accaccactg atccggctgc taagatagct
     6661 tgacctgtga agtgaaaaat ggcgcacatt gtgcgacatt ttttttgtct gccgtttacc
     6721 gctactgcgt cacggatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6961 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7021 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7081 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7141 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcag
     7201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7321 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7381 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7441 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7501 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7561 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7621 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7681 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7741 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7801 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7861 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7921 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7981 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8041 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8101 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8161 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9001 ctcacatg
//
