LOCUS       pIEx_Bac-4.        7496 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell and baculovirus vector encoding a cleavable N-terminal 
            GST tag and a C-terminal 10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx Bac-4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1196..1678
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        1682..2273
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        2283..2392
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2435..3088
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             3119..3133
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3133..3179
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3203..3229
                     /label=9xHis
                     /note="9xHis affinity tag"
     terminator      3265..3571
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_recomb     3600..4957
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5220..5808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5982..6839)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6840..6944)
                     /label=AmpR promoter
     rep_origin      6971..7426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
     1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
     1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
     1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
     1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
     1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
     1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
     1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
     1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
     1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
     1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
     1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
     1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
     1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
     1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
     2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
     2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
     2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
     2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
     2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga
     2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa
     2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcaga
     3121 tgacgacgac aaggtaccgg atccgacgtc ggtcgacaag cttgcggccg caggagctcc
     3181 gggcttctcc tcaatttccg ctcatcacca ccatcatcac catcaccacc actaagtgat
     3241 taacctcacg acctaggcct caggttatac atatattttg aatttaatta attatacata
     3301 tattttatat tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca
     3361 tagaaataac aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt
     3421 tcatcatgta tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca
     3481 acaaaatgat aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga
     3541 ctaaaccttc atcgcccagt aaccaatcta ggtagctgag cgcatgcaag ctgatccggg
     3601 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     3661 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     3721 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     3781 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     3841 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     3901 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     3961 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     4021 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
     4081 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
     4141 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
     4201 aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
     4261 ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
     4321 tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
     4381 ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
     4441 aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt
     4501 ttttggtttg accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa
     4561 ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg
     4621 cggcaattca gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg
     4681 agaaggtggt ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac
     4741 gattgtgggc accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg
     4801 cagcgcttgg ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc
     4861 tataagcatt gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta
     4921 agcaattgta ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa
     4981 gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgaggttta
     5041 aacgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5641 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6961 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7381 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc
     7441 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca
//
