LOCUS       pIEx_Bac-3.        6802 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell and baculovirus vector encoding a cleavable N-terminal 
            10xHis tag and a cleavable C-terminal Strep-Tag(R) II.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx Bac-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1196..1678
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        1682..2273
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        2283..2392
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2394..2396
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2400..2426
                     /label=9xHis
                     /note="9xHis affinity tag"
     CDS             2436..2459
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    2457..2501
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2505..2522
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             2529..2552
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      2571..2877
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_recomb     2906..4263
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(4526..5114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5288..6145)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6146..6250)
                     /label=AmpR promoter
     rep_origin      6277..6732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
     1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
     1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
     1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
     1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
     1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
     1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
     1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
     1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
     1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
     1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
     1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
     1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
     1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
     1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
     2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
     2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
     2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
     2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
     2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcac
     2401 atcaccacca tcatcaccat caccaccacg gtgcacttga agtcctcttt cagggacccg
     2461 ggtaccagga tcctgtacaa gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta
     2521 gttccgcttg gagccacccg cagttcgaaa agtaagtgat taacctcagg ttatacatat
     2581 attttgaatt taattaatta tacatatatt ttatattatt tttgtctttt attatcgagg
     2641 ggccgttgtt ggtgtggggt tttgcataga aataacaatg ggagttggcg acgttgctgc
     2701 gccaacacca cctcccttcc ctcctttcat catgtatctg tagataaaat aaaatattaa
     2761 acctaaaaac aagaccgcgc ctatcaacaa aatgataggc attaacttgc cgctgacgct
     2821 gtcactaacg ttggacgatt tgccgactaa accttcatcg cccagtaacc aatctaggta
     2881 gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg
     2941 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
     3001 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
     3061 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
     3121 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
     3181 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
     3241 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
     3301 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg
     3361 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
     3421 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
     3481 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
     3541 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
     3601 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
     3661 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
     3721 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
     3781 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
     3841 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
     3901 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
     3961 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
     4021 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
     4081 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
     4141 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
     4201 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
     4261 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
     4321 agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg ctgcgctcgg
     4381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6721 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6781 gggcctcttc gctattacgc ca
//
