LOCUS       pIEx_Bac-1.        6796 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell and baculovirus vector encoding a cleavable N-terminal 
            Strep-Tag(R) II and a C-terminal 10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx Bac-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1196..1678
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        1682..2273
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        2283..2392
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2394..2396
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2403..2426
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             2433..2447
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    2447..2506
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2517..2543
                     /label=9xHis
                     /note="9xHis affinity tag"
     terminator      2565..2871
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_recomb     2900..4257
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(4520..5108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5282..6139)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6140..6244)
                     /label=AmpR promoter
     rep_origin      6271..6726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
     1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
     1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
     1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
     1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
     1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
     1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
     1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
     1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
     1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
     1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
     1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
     1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
     1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
     1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
     2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
     2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
     2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
     2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
     2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcaa
     2401 gctggagcca cccgcagttc gaaaagggtg cagatgacga cgacaaggta ccggatccga
     2461 cgtcggtcga caagcttgcg gccgcaggag ctccgggctt ctcctcaatt tccgctcatc
     2521 accaccatca tcaccatcac caccactaag tgattaacct caggttatac atatattttg
     2581 aatttaatta attatacata tattttatat tatttttgtc ttttattatc gaggggccgt
     2641 tgttggtgtg gggttttgca tagaaataac aatgggagtt ggcgacgttg ctgcgccaac
     2701 accacctccc ttccctcctt tcatcatgta tctgtagata aaataaaata ttaaacctaa
     2761 aaacaagacc gcgcctatca acaaaatgat aggcattaac ttgccgctga cgctgtcact
     2821 aacgttggac gatttgccga ctaaaccttc atcgcccagt aaccaatcta ggtagctgag
     2881 cgcatgcaag ctgatccggg ttattagtac atttattaag cgctagattc tgtgcgttgt
     2941 tgatttacag acaattgttg tacgtatttt aataattcat taaatttata atctttaggg
     3001 tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt
     3061 aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc
     3121 gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct
     3181 tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg
     3241 acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat
     3301 tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct
     3361 ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac
     3421 gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa
     3481 tttgtagttg agctttttgg aattatttct gattgcgggc gtttttgggc gggtttcaat
     3541 ctaactgtgc ccgattttaa ttcagacaac acgttagaaa gcgatggtgc aggcggtggt
     3601 aacatttcag acggcaaatc tactaatggc ggcggtggtg gagctgatga taaatctacc
     3661 atcggtggag gcgcaggcgg ggctggcggc ggaggcggag gcggaggtgg tggcggtgat
     3721 gcagacggcg gtttaggctc aaatgtctct ttaggcaaca cagtcggcac ctcaactatt
     3781 gtactggttt cgggcgccgt ttttggtttg accggtctga gacgagtgcg atttttttcg
     3841 tttctaatag cttccaacaa ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc
     3901 gtcggcattg gtggagcggg cggcaattca gacatcgatg gtggtggtgg tggtggaggc
     3961 gctggaatgt taggcacggg agaaggtggt ggcggcggtg ccgccggtat aatttgttct
     4021 ggtttagttt gttcgcgcac gattgtgggc accggcgcag gcgccgctgg ctgcacaacg
     4081 gaaggtcgtc tgcttcgagg cagcgcttgg ggtggtggca attcaatatt ataattggaa
     4141 tacaaatcgt aaaaatctgc tataagcatt gtaatttcgc tatcgtttac cgtgccgata
     4201 tttaacaacc gctcaatgta agcaattgta ttgtaaagag attgtctcaa gctcggatcg
     4261 atcccgcacg ccgataacaa gccttttcat ttttactaca gcattgtagt ggcgagacac
     4321 ttcgctgtcg tcgaggttta aacgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4381 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4441 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4921 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4981 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5221 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5401 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5581 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5881 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6241 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     6301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6661 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     6721 caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     6781 cttcgctatt acgcca
//
