LOCUS       pIEx-9.        3747 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of proteins with a 
            cleavable N-terminal Strep-Tag(R) II plus a cleavable C-terminal 
            10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     CDS             1084..1086
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1093..1116
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1123..1146
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    1148..1188
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1192..1209
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1216..1242
                     /label=9xHis
                     /note="9xHis affinity tag"
     terminator      1264..1571
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1601..1705
                     /label=AmpR promoter
     CDS             1706..2563
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2737..3325
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3613..3634
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3649..3679
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3687..3703
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3711..3727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggcaa gctggagcca cccgcagttc gaaaagggtg cacttgaagt cctctttcag
     1141 ggacccgggt accaggatcc tgtacaagtc gacgcggccg cagagctcgc tctggtgcca
     1201 cgcggtagtt ccgctcatca ccaccatcat caccatcacc accactaagt gattaacctc
     1261 aggttataca tatattttga atttaattaa ttatacatat attttatatt atttttgtct
     1321 tttattatcg aggggccgtt gttggtgtgg ggttttgcat agaaataaca atgggagttg
     1381 gcgacgttgc tgcgccaaca ccacctccct tccctccttt catcatgtat ctgtagataa
     1441 aataaaatat taaacctaaa aacaagaccg cgcctatcaa caaaatgata ggcattaact
     1501 tgccgctgac gctgtcacta acgttggacg atttgccgac taaaccttca tcgcccagta
     1561 accaatctag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2881 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3721 ctatgaccat gattacgaat tcccggg
//
