LOCUS       pIEx-1.        3897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of proteins with a 
            cleavable N-terminal 10xHis-S-Tag cassette plus a C-terminal HSV 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     CDS             1084..1086
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1090..1116
                     /label=9xHis
                     /note="9xHis affinity tag"
     CDS             1129..1173
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1189..1206
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1225..1239
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1354..1386
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     terminator      1414..1721
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1751..1855
                     /label=AmpR promoter
     CDS             1856..2713
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2887..3475
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3763..3784
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3799..3829
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3837..3853
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3861..3877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggcac accaccatca ccaccatcac caccatcact cttctggtaa agaaaccgct
     1141 gctgcgaaat ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg
     1201 ggcagcgcag gttctggtac gattgatgac gacgacaaga gtccgggctt ctcctcaacg
     1261 gatcccaatt gggagctcgt gtacacggcg cgcctgcagg tcgacggtac cggttcgaag
     1321 cttgcggccg cacagctgta tacacgtgca agccagccag aactcgcccc ggaagacccc
     1381 gaggatctcg agcactaagt gattaacctc aggttataca tatattttga atttaattaa
     1441 ttatacatat attttatatt atttttgtct tttattatcg aggggccgtt gttggtgtgg
     1501 ggttttgcat agaaataaca atgggagttg gcgacgttgc tgcgccaaca ccacctccct
     1561 tccctccttt catcatgtat ctgtagataa aataaaatat taaacctaaa aacaagaccg
     1621 cgcctatcaa caaaatgata ggcattaact tgccgctgac gctgtcacta acgttggacg
     1681 atttgccgac taaaccttca tcgcccagta accaatctag acgtcaggtg gcacttttcg
     1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3721 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3781 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3841 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat tcccggg
//
