LOCUS       40924_25336        5969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIE1-LUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Intron-dependent stimulation of marker gene expression in cultured 
            insect cells
  JOURNAL   Insect Mol. Biol. 11 (1), 87-95 (2002)
  PUBMED    11841506
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2001) Medical Entomology Section, Laboratory of 
            Parasitic Diseases, National Institutes of Health, 4 Center Drive 
            MSC 0425, Bethesda, MD 20892-0425, USA
REFERENCE   3  (bases 1 to 5969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2002"; volume: "11"; issue: "1"; pages: "87-95"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2001) Medical Entomology Section, Laboratory of Parasitic 
            Diseases, National Institutes of Health, 4 Center Drive MSC 0425, 
            Bethesda, MD 20892-0425, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..595
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     regulatory      615..1201
                     /note="Autographa californica nuclear polyhedrosis virus 
                     immediate early promoter"
                     /regulatory_class="promoter"
     CDS             1328..2977
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3146..3251
                     /note="Heliothis virescens p63 chaperonin gene double 
                     poly-adenylation sequence"
     CDS             4168..5025
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5199..5787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca atttaaatcg ggcccaacta gtggatccgg cgcgtaaaac
      121 acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat aaccgaactg
      181 gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatggtc atttgttttt
      241 caaatcgaga tgatgtcatg ttttgcacac gggctcataa actgctttac gagtagaatt
      301 ctacgtgtaa cgcacgatcg agatgagtca tttgttttgc aatatgatat catacaatat
      361 gactcatttg tttttcaaaa ccgaacttga tttacgggta gaattctact cgtaaagcac
      421 aatcaaaaag atgatgtcat ttgtttttca aaactgaact ctcggcttta cgagtagaat
      481 tctacgtgta aaacacaatc aagaaatgat gtcatttgtt ataaaaataa aagctgatgt
      541 catgttttgc acatggctca taactaaact cgctttacgg gtagaattct acgcgccgga
      601 tccactagct agttctagag tcgcgatgtc tttgtgatgc gccgacattt ttgtaggtta
      661 ttgataaaat gaacggatac agttgcccga cattatcatt aaatccttgg cgtagaattt
      721 gtcgggtcca ttgtccgtgt gcgctagcat gcccgctaac ggacctcgta cttttggctt
      781 caaaggtttt gcgcacagac aaaatgtgcc acacttgcag ctctgcatgt gtgcgcgtta
      841 ccacaaatcc caacggcgca gtgtacttgt tgtatgcaaa taaatctcga taaaggcgcg
      901 gcgcgcgaat gcagctgatc acgtacgctc ctcgtgttcc gttcaaggac ggtgttatcg
      961 acctcagatt aatgtttatc ggccgactgt tttcgtatcc gctcaccaaa cgcgtttttg
     1021 cattaacatt gtatgtcggc ggatgttcta tatctaattt gaataaataa acgataaccg
     1081 cgttggtttt agagggcata ataaaagaaa tattgttatc gtgttcgcca ttagggcagt
     1141 ataaattgac gttcatgttg gatattgttt cagttgcaag ttgacactgg cggcgacaag
     1201 agatctcgtt aacttctaga gggtaccact gcaggtcgac tctagaggat ccgagcttgg
     1261 aattcctttg tgttacattc ttgaatgtcg ctcgcagtga cattagcatt ccggtactgt
     1321 tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctctaga
     1381 ggatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
     1441 aacaattgct tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga
     1501 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
     1561 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
     1621 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
     1681 gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa
     1741 cgtgcaaaaa aaattaccaa taatccagaa aattattatc atggattcta aaacggatta
     1801 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
     1861 atacgatttt gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaattc
     1921 ctctggatct actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag
     1981 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
     2041 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     2101 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca
     2161 ggattacaaa attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag
     2221 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc
     2281 tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca
     2341 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     2401 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     2461 cgggaaaacg ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat
     2521 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     2581 acattctgga gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt
     2641 gaagtcttta attaaataca aaggatatca ggtggccccc gctgaattgg aatcgatatt
     2701 gttacaacac cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg
     2761 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     2821 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     2881 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     2941 cctcataaag gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga
     3001 tgacgaaatt cttagctatt gtaatattat atgcaaattg atgaatggta attttgtaat
     3061 tgtgggtcac tgtactattt taacgaataa taaaatcagg tataggtaac taaaaaggaa
     3121 ttcgagctcg atcgatacac gtgctgtaca catttacttt aaatttaata aaatttactt
     3181 tagccgttgt ccgataattc ttatatttaa tttaaaccac ctgcaagctt ttaataaatc
     3241 tatatgttcc cgggcgttta aacgcggccg ctatgcagga ttttatgtat gaaaacgccc
     3301 accattccca cccttctggg gccggacggc atgacatcgc tgcgcgaata tgccggttat
     3361 cacggcggtg gcagcggatt tggagggcag ttgcggtcgt ggaacccacc gagtgaaagt
     3421 gtggatgcag ccctgttgcc caactttacc cgtggcaatg cccgcgcaga cgatctggta
     3481 cgcaataacg gctatgccgc caacgccatc cagctgcatc aggatcatat cgtcgggtct
     3541 tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc
     3601 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc
     3661 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
     3721 cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt
     3781 ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc
     3841 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat
     3901 tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt
     3961 gagttacccg gcgggcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag
     4021 actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
     4081 catgaaacaa ttcaaacagg gttctggcgt cgttctcgta ctgttttccc caggccagtg
     4141 ctttagcgtt attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4201 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4261 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4321 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4381 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4441 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4501 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4561 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4621 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4681 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4741 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4801 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4861 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4921 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4981 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5041 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5101 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5341 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5821 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5881 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5941 agcgagtcag tgagcgagga agcggaaga
//
