LOCUS       pIDS.        2231 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector with a p10 promoter and a spectinomycin 
            resistance marker. Serves as a donor vector in the MultiBac(TM) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pIDS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    133..196
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    261..309
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    511..544
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      546..902
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             1365..2153
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgagc catggtgcta gcagctgatg
      181 catagcatgc ggtaccggga gatgggggag gctaactgaa acacggaagg agacaatacc
      241 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
      301 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
      361 gaccccattg ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt
      421 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacta
      481 cgggtacgtt taaacccatg tgcctggcag ataacttcgt ataatgtatg ctatacgaag
      541 ttatggtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa
      601 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg
      661 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa
      721 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa
      781 ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact
      841 gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga
      901 cataattcag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg
      961 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctgggag
     1021 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     1081 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1261 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1321 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     1381 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1441 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1501 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1561 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1621 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1681 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1741 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1801 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1861 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1921 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1981 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2041 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2101 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     2161 taacaattcg ttcaagccga cggatctatg tcgggtgcgg agaaagaggt aatgaaatgg
     2221 cacctaggta t
//
