LOCUS       pIB_V5-His.        3521 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for transient or stable constitutive expression 
            of C-terminally V5-6xHis-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pIB V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    557..652
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             659..700
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             710..727
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    745..874
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1002..1590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1665..1956
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        1982..2029
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2048..2443
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2566..3423
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg
       61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt
      121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
      181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
      241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
      301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
      361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
      421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
      481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
      541 catctgttcg aatttaaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      601 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
      661 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
      721 tcaccattga gtttatctga ctaaatctta gtttgtattg tcatgtttta atacaatatg
      781 ttatgtttaa atatgttttt aataaatttt ataaaataat ttcaactttt attgtaacaa
      841 cattgtccat ttacacactc ctttcaagcg cgtgggatcg atgctcactc aaaggcggta
      901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcgaaa cacgcacggc
     1681 gcgcgcacgc agcttagcac aaacgcgtcg ttgcacgcgc ccaccgctaa ccgcaggcca
     1741 atcggtcggc cggcctcata tccgctcacc agccgcgtcc tatcgggcgc ggcttccgcg
     1801 cccattttga ataaataaac gataacgccg ttggtggcgt gaggcatgta aaaggttaca
     1861 tcattatctt gttcgccatc cggttggtat aaatagacgt tcatgttggt ttttgtttca
     1921 gttgcaagtt ggctgcggcg cgcgcagcac ctttgccggg atctgccggg ctgcagcacg
     1981 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2041 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     2101 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     2161 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgcgcaga
     2221 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     2281 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggttcttct
     2341 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     2401 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg
     2461 aggagcagga ctgacacgtc ccgggagatc tgcatgtcta ctaaactcac aaattagagc
     2521 ttcaatttaa ttatatcagt tattacccat tgaaaaagga agagtatgag tattcaacat
     2581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3481 taatttaaaa ggatctaggt gaagatcctt tttgataatc t
//
