LOCUS       40924_25291        4269 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIB-NC-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Tatematsu K-i.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2013) Transgenic Silkworm Research Unit, National 
            Institute of Agrobiological Sciences (NIAS), Ohwashi 1-2, Tsukuba, 
            Ibaraki 305-8634, Japan
REFERENCE   2  (bases 1 to 4269)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2013) Transgenic Silkworm Research Unit, National Institute 
            of Agrobiological Sciences (NIAS), Ohwashi 1-2, Tsukuba, Ibaraki 
            305-8634, Japan"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    569..578
                     /label=NC (negative consensus context sequence)
                     /note="NC (negative consensus context sequence)"
     CDS             579..1295
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1407..1448
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1458..1475
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    1493..1622
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1750..2338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2413..2704
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2730..2777
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2796..3191
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3314..4171
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg
       61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt
      121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
      181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
      241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
      301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
      361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
      421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
      481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
      541 catctgttcg aatttaaagc ttcctagccc ggcggcgtat ggtttcgaag ggcgaggagc
      601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      661 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      721 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      781 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      841 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      901 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      961 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1021 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1081 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1141 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1201 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1261 ccgggatcac tctcggcatg gacgagctgt acaagtaagc tagggtcgac ggtaccgagc
     1321 tcggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct
     1381 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     1441 attctacgcg taccggtcat catcaccatc accattgagt ttatctgact aaatcttagt
     1501 ttgtattgtc atgttttaat acaatatgtt atgtttaaat atgtttttaa taaattttat
     1561 aaaataattt caacttttat tgtaacaaca ttgtccattt acacactcct ttcaagcgcg
     1621 tgggatcgat gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2401 gattttggtc atgcgaaaca cgcacggcgc gcgcacgcag cttagcacaa acgcgtcgtt
     2461 gcacgcgccc accgctaacc gcaggccaat cggtcggccg gcctcatatc cgctcaccag
     2521 ccgcgtccta tcgggcgcgg cttccgcgcc cattttgaat aaataaacga taacgccgtt
     2581 ggtggcgtga ggcatgtaaa aggttacatc attatcttgt tcgccatccg gttggtataa
     2641 atagacgttc atgttggttt ttgtttcagt tgcaagttgg ctgcggcgcg cgcagcacct
     2701 ttgccgggat ctgccgggct gcagcacgtg ttgacaatta atcatcggca tagtatatcg
     2761 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     2821 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     2881 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     2941 atcattttac tgggggacct tgcgcagaac tcgtggtgct gggcactgct gctgctgcgg
     3001 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     3061 cctgcggacg gtgccgacag gttcttctcg atctgcatcc tgggatcaaa gccatagtga
     3121 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     3181 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtccc gggagatctg
     3241 catgtctact aaactcacaa attagagctt caatttaatt atatcagtta ttacccattg
     3301 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3361 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3421 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3481 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3541 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3601 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3661 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3721 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3781 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3841 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3901 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3961 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4021 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4081 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4141 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4261 tgataatct
//
