LOCUS       40924_25286       10550 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pI2zCXCR4b6.6kbprom_mCherryF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Gamba L, Cubedo N, Ghysen A, Lutfalla G, Dambly-Chaudiere C.
  TITLE     Estrogen receptor ESR1 controls cell migration by repressing 
            chemokine receptor CXCR4 in the zebrafish posterior lateral line 
            system
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 107 (14), 6358-6363 (2010)
  PUBMED    20308561
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Gamba L, Cubedo N, Ghysen A, Lutfalla G, Dambly-Chaudiere C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2010) DIMNP, CNRS/Universite Montpellier2, Place 
            Eugene Bataillon, Montpellier, Cedex5 F-34095, France
REFERENCE   3  (bases 1 to 10550)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2010"; volume: "107"; issue: "14"; pages:
            "6358-6363"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2010) DIMNP, CNRS/Universite Montpellier2, Place Eugene 
            Bataillon, Montpellier, Cedex5 F-34095, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    656..673
                     /label=I-SceI meganuclease site
                     /note="I-SceI meganuclease site"
     CDS             complement(5657..5668)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      6647..6791
                     /gene="CXCR4b"
                     /label=derived from zebrafish
                     /note="derived from zebrafish"
                     /regulatory_class="promoter"
     regulatory      6745..6791
                     /gene="CXCR4b"
                     /note="minimal promoter; derived from zebrafish"
                     /regulatory_class="promoter"
     exon            6792..6895
                     /gene="CXCR4b"
                     /number=1
                     /note="derived from zebrafish"
     misc_difference 6881
                     /gene="CXCR4b"
                     /replace="a"
                     /label=ATG start codon mutated to GTG
                     /note="ATG start codon mutated to GTG"
     intron          6896..7247
                     /gene="CXCR4b"
                     /number=1
                     /note="derived from zebrafish"
     exon            7248..7261
                     /gene="CXCR4b"
                     /number=2
                     /note="derived from zebrafish"
     CDS             7290..7976
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     polyA_signal    8191..8312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8323..8340
                     /label=I-SceI meganuclease site
                     /note="I-SceI meganuclease site"
     promoter        complement(8359..8377)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8398..8414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8422..8438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8446..8476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8491..8512)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8800..9388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9562..10419)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10420..10524)
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtactaggg
      661 ataacagggt aatctcgaga agcttgataa ggcctgtcac agctcattgc atgttactca
      721 tggtaataat taatcataat tcttatttct ggatgtgtta ttgttgactt tggatgagaa
      781 attaaatgca aaaacatata ttgttttgac taaaatgtca gattatgtaa tgtgtttatg
      841 tgcttcctgc tagcacatct aagaggtgta aatatgttaa cagccacagt tgtgaatagt
      901 agtgtgacag tttgaactga aaacaattca attgcagatt agttctaaat gctgtttccc
      961 accatgcagt caatattatg actgagtata aatatatact tatgataaat actaaaagta
     1021 ttagtctagt acatggttag tataaatatt aacccgaata atactaaaat aatgtatgaa
     1081 gattgtgtgt taagaatact taaaccacaa aaacatgatt aaactggtta ataactgaag
     1141 tgttcacatg cagaaacctt taagttagat ctggattttt ttttaaatat gcatttctct
     1201 cagcctcctt tgtttatgtt cagttatttc acattaaagg ctatgaaaat aacctattat
     1261 tttccatcca ttaattaatt tcctctccat ttcagagatc acctcagcag aatgaacagc
     1321 caactatttc agccacaacc cagtattgag aaacacccat atccactcat tcacagacac
     1381 actcatacat atggacaatt cagcttatcc aatttaccta tactgcatgt ctttggactg
     1441 tgggggaaac ccagaggaaa cccacacgaa catggggtga acatgcaaac tccacataga
     1501 aatgccaacg agcccagctg ggactcgaac aagtgacatt cttgctgtga ggcaacaatg
     1561 ctaaagactg agccatatcc aggttaacag agaggaccgg gagaattctc tgtgggccgg
     1621 tccatatttt ggccgtgagg gctgggctgg tgtcatatcc tcctgagacc aaaataaatc
     1681 ttttttcttc tttttttctg taatttccaa catcctttgg tttacaaaaa atctataatt
     1741 tagagttttg acattttgtt tttaatttta attttacagg atgtccactg tagtggacca
     1801 caggaccatt tcagttcgaa atgaccacca ctcagacaac aaaactgtac aggatgtggc
     1861 tgtaaagaga tattaatcca tattaatgta acaaaatcaa acaaaaaaaa acattttttt
     1921 ccatttgtat tgaagttcct gaaacagttt agtctgaaag agaactcaaa aaaaaaaaaa
     1981 aaaagaaaga agtgatgtta atccaaaaga aatttaacat aaaaacgatt gtcattatct
     2041 aattgtctct aattctctaa ttgtctgatt gtcagactct aaatcagagt ccccctaact
     2101 gtcttttaaa gaatcctctt tgcttcagtt ctgcccccta caacatgcat caataattac
     2161 attaagatgg ttctggtgtc cactatagtg gacattttaa aaaggatgat attttaaacc
     2221 acaaacaagt taacagcttt caaagctaaa tgtattgttc ctgacacttt aatacataaa
     2281 tataattttt gtaataacaa tagtaaaaaa gaaaaagaaa aaaaaaacat ttaaaaaact
     2341 acattttaca tacttctctc cttcccataa aatttgcttt tttgtgagtc ggccattttg
     2401 gatgcagtgt aagaagtggt tcccagcatg ccagccaatg aaatgacata tcactaaaaa
     2461 aaactcagga tgttctctga acagtacaac aggacttgac agagtagctc aaagaattca
     2521 aagaaaactc atgaaaacgc aatgtccact ccaaaggact caagtctaca ggtggggttt
     2581 ctggaggctt tagctgcctg cactctcaac agtcacaatt tgttttccat ttatttatat
     2641 attttaccat agcctcacta tttttattca ttattttacc acagctccac tctttttctt
     2701 tattatattg cagcctcata agcagaatgc agcctgcagg ccaacagcgg tacccaacac
     2761 aatctcacgg caattcgtaa cttttccatt tagcggctaa ttcgtacaaa ttcgtaagat
     2821 ctaattcgta cattttagta cgatttgctc atcacccaat gacggttggg tttaggggtg
     2881 gggttaggtg ccacgcctcc tttttaaaat cgtacatttt cttacgactg aactcgtacg
     2941 aaatcgtaca aattagccac taaactgcca aaacgtaaaa tacttacgtt ttctcgtgag
     3001 atcaggctgc gataccttgc tcatcagcac aaaggtggca ctaaaaggcc agaattgaaa
     3061 aaagctgggt atattttttt gttaaaacca gcggcagcaa gagtacaaca acacatgaaa
     3121 atgatgaaac agccggtaag ttaacattag ctggttagga gcagtgcaac acaacaatgt
     3181 ttgcagttta tgtaccgaag gtttacttgc tcttcagcag cagcagcagc agcctcttgt
     3241 ccggtttgct gctaacagtg aagagccagg ccccgtaaca ttaccaagca ccagtcatga
     3301 gcccaacaca ctagaatggg caggtaggat ttttatttag tgaagagttc aaattattat
     3361 taatattaat tttcccagtg ttggattttg cttggacgag catctgctgc ataaaacatg
     3421 tgctgtataa gttggcagtt cataccgctg tggcgacccc tgattaataa atggactaag
     3481 ctaaaaagaa aataaattaa tgaattaata ttaatgaata ttaaaaatcc tcaatgaaaa
     3541 aatgccctgg ctgagacaat taatgaaatc cgatgattca ctacattaat aaatctgact
     3601 taacttgatc agaaagctaa aaatggggaa taaagaaaaa gccgtcatta gaaagtaata
     3661 ctgtggttat aatgtctggc aatacctatt atttaattgt gttaaagaac tggatatcta
     3721 taaacaattt tgcacataat atatattcag atattgcaga aaaaggacac tgcgatgttt
     3781 tcaagaatag tgttggagat tcagctactc tttaattttc tcatatttat gaaaaatatt
     3841 tgaagtgata aagcagctgt ttttttgaaa aagacatgat agtgtcatta gaaagtttaa
     3901 tacttttaat tttaatcttt atttcatata tttagcttaa tatttgtatt tatttgaggt
     3961 ttttttaatt tcaaggtttg gatatttatg agcacttatt cttacagaaa atagattctg
     4021 aacattacat tgtgataact gttcaaataa atcttcactg taaagtactg atttgaaaat
     4081 gatgttattt taatatagtc agttgtgaac tgaagtgggc aggtctaagg tttaaaactt
     4141 aggtttaaaa attgctgaaa atgtgtccaa ctccggctct ggccatacct ctattctttt
     4201 caatacaagt gaaattaatg aacatgcaca gggaagcctg gcaaaaatgc agaaaatttt
     4261 gcacctgaac tcttcaactc tatcctctat ttaacttcaa gctctgctat ttgctttatt
     4321 tttttttaat ccaagcatgt ttcttcatga cactaactaa ataaaaagca gctgacgttg
     4381 gctatcaagc ttcattttct tttcagctta atgaaccgcc aacttatcca tgtagcatat
     4441 ttattacaca gcggatgccc taccagctgc aaccaatctc tgactctccc acaaatgcac
     4501 agagactccc ggatgcccga ataacccacc tggtaacttc tgggagactt gggatgtttg
     4561 gaataacctc ccgcccgact tatatatcaa aattctctct tcataaagtc gtattacatt
     4621 tccataaaac actgtgatat agtcgtgctc gcatcgtatc gcttttctta ctacaattga
     4681 tccactccaa agttagtttc aatcgagcag agaccacctc attcaggcga tctcagaccg
     4741 attgttttgg cgcaaatccg agcgggattg ctgaaattcc atatgccaaa tgaaccgcgc
     4801 taactgggca aatgagacaa aagtctaggt gtgaaagcac ccaaaattgg ctgtagtgta
     4861 tgtgagagtg tatgggtgtt tcccagtact aggttgcagc tggaagggca tccgctgcgt
     4921 aaagcatatg ctagatgagt tgatggttca tttcactgtg gcgaccccag attattaaaa
     4981 taactaagcc aatagaaaat gaattaataa ataattaaaa tattagccaa ctctgaactc
     5041 gcattcggca aactctcaca aatgaaatga ttaactccct taaatcagcg cgtcatgttt
     5101 cagagaaata tgatcatcgt taggagtcgc agagtttgga tctgtcagga aatgttagtt
     5161 taggcttatg atggaggcga ctgggtgacc ttcttccttc atcactcaca atcagagccc
     5221 agcattaaat atattgcagg aaggaccaga ggagaaccgt catgcagtaa attacactac
     5281 aaagcctgat gtgtcctcaa gtaacggcct tcacactgct cttcacatga cgcgataata
     5341 aacaaactgc tgggatttct gagagagaaa gtccactaca gatgatctga tgaatatgtg
     5401 agggatcttg tgaagccttt aacacacaat aaaccctggc agggtgatca gcacttcaac
     5461 acaacaaagt ggcttgtatt gcccttgagg gaattaattt gtgataatga cctgctgact
     5521 gctcattatc ccctttgttg cacagctaat tagcaaataa ataaataaat aaattgacat
     5581 caaataatac tttgaatata aaagatatct atccgtctga ttgtctgtct gtctttccgt
     5641 ttgtctgcct gtctgttcgt ccttctattt atctaccttt ttcattatct atctatctat
     5701 ctatctatct atctatctat ctatctatct atctatctat ctatctatct atctatctat
     5761 ctatctatct gtctgtctgt ctgtctgtct gtctgtctgt ctgtctgtct gtctgtctgt
     5821 ctgtctgtct gtctgtctgt ctgtctgtct gtctgtctgt ctgtctatct atctatctat
     5881 ctatctatct atctatcata tatctattat ctttttttca ttatctatct atctatctat
     5941 ctatctatct atctatctat ctatctatct atctatctat ctatctatct atctatctat
     6001 ctatctatct atctatctat catctttttt cattatctat ctgtctaaga tgatagcaat
     6061 ctatagtaaa taatacattg ttttcaaacc aaactatagt aatgtagccc acttaaattg
     6121 atctggtgtt gtttttgtat agttggtatg gaaactgtgg tagtaatctt tgatctgttg
     6181 ttttgattct gcagttgcta tagcaacaca acgaaacagg aatataaaca aatgacatgc
     6241 tgtagttttc tacaacaata gggtatacta tactggtaca ctacagttta ctgtagtaaa
     6301 aactaaaata ggatatactg cctttttctt ttttctcttt tgttttttac agttgaacag
     6361 ttctctacag tgctgaagca ttcattaaca aagttgaaat actatatgat ttacaataaa
     6421 ataaacttta ctataaaata gttcaaaaac agttttattt tttataatta ggctactata
     6481 ccgttttttt catgtagtaa aaaaaagcaa atcgtattaa aaatattttt tcttcaaaac
     6541 attgttcaat agtgtttttc aaaccatgtt tttttctgtg cataggctat ttttgctatc
     6601 tagcccgaat aaacatgtag atattacgct tcctctgtaa aatccgctcg agcgcatgca
     6661 tttaacaccg tttattttga catgccagag agtactctct catcaccccc cacacaccca
     6721 caacgcacaa gcgccgcctg catcatgacc ccgccctgct gaataaaatt gggcgtgtct
     6781 cagggaacgg aggactattt aaatgctgct gtgagaggag ggcggttgac tgaagtgtgg
     6841 agacttattg cgccttttta agcacaccta taagaacaaa gtggaatttt acgatgtaag
     6901 ttggcttgtg attaatttat aacgctatgt tatcagacat tccattgata agtattatta
     6961 ataagactag ttaaagtgta tcatgtttta ttacttgccc tagggtgtca cgttttacca
     7021 ttcaggctcg tgtttggaag cattggaaaa taactatata tttttttcag ttatttattt
     7081 attttttgtc atttattatt aaccttgtat ttgtataaaa ccattttact ttttttgcat
     7141 ttgttttctt gaattattat tattttttgt tagtattatc gtaataaagt ggtatttata
     7201 taaaagttaa tcaaaatact gatttttttt tcttcttttt tctgcagagc atcattttag
     7261 acgcggccgc atgcatggat cccgccacca tggtgagcaa gggcgaggag gataacatgg
     7321 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     7381 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     7441 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     7501 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     7561 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     7621 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     7681 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     7741 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     7801 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     7861 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     7921 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcgcct
     7981 ccggactcag atctaagctg aaccctcctg atgagagtgg ccccggctgc atgagctgca
     8041 agtgtgtgct ctcctgagga tcgatccacc ggatctagat aactgatcat aatcagccat
     8101 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     8161 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     8221 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8281 tgtggtttgt ccaaactcat caatgtatct taacgcgtta agattaccct gttatcccta
     8341 gagctccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
     8401 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg
     8461 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt
     8521 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8581 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tcggcccccc
     8821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8881 aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     9001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9241 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9601 tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga
     9661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9961 catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10021 ggccgcagtg ttatcactca tgcttatggc agcactgcat aattctctta ctgtcatgcc
    10081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
