LOCUS       62056_12675        4844 bp DNA     circular SYN 29-APR-2019
DEFINITION  Expression vector pHytru, complete sequence.
ACCESSION   MK501803
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Chiera JM, Finer JJ, Grabau EA.
  TITLE     Ectopic expression of a soybean phytase in developing seeds of 
            Glycine max to improve phosphorus availability
  JOURNAL   Plant Mol. Biol. 56 (6), 895-904 (2004)
  PUBMED    15821988
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Gunadi A, Finer JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2019) Horticulture and Crop Science, The Ohio 
            State University, Ohio Agricultural Research and Development Center,
            1680 Madison Ave., Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2004"; volume: "56"; issue: "6"; pages: "895-904"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2019) Horticulture and Crop Science, The Ohio State 
            University, Ohio Agricultural Research and Development Center, 1680 
            Madison Ave., Wooster, OH 44691, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..53
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        68..98
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..122
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     130..146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        670..1015
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1039..2061
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      2111..2363
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2372..2388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2864..2968
                     /label=AmpR promoter
     CDS             2969..3826
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4000..4588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
       61 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      121 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcc
      181 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
      241 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
      301 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
      361 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
      421 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
      481 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
      541 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
      601 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
      661 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
      721 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
      781 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      841 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      901 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      961 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     1021 gactctagag gatccgatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     1081 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     1141 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     1201 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     1261 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     1321 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     1381 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     1441 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     1501 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     1561 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     1621 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     1681 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     1741 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     1801 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     1861 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     1921 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     1981 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     2041 actcgtccga gggcaaagga atagagtaga tgccgaccgg gatccccggg taccgagctc
     2101 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     2161 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     2221 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     2281 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     2341 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact
     2401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2521 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2581 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2641 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2701 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2761 gcgcgaggga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2821 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4141 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4621 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4681 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4741 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4801 gcgttggccg attcattaat gcagctggca cgacaggttt cccg
//
