LOCUS       62056_12650        7597 bp DNA     circular SYN 24-FEB-2021
DEFINITION  Cloning vector pHyp4s kanamycin resistance protein gene, complete 
            cds.
ACCESSION   MW132123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition 
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University, 
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes 
            Ave, Pittsburgh, PA 15219-3016, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label=PnptII
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1916
                     /label=hypothetical protein 4 secretion signal
                     /note="hypothetical protein 4 secretion signal"
     misc_feature    1926..2150
                     /label=scorpine
                     /note="scorpine"
     misc_feature    2217..3554
                     /label=alkaline phosphatase, no leader signal
                     /note="alkaline phosphatase, no leader signal"
     CDS             complement(5146..5805)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5806..6575)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgg tttcttttcc ctgccgactc gtcgcctcga
     1801 cggtacttct ggcatcgggc ctgagcgccc tgcctgccat ggccgcatcg aagcagcagg
     1861 cgctggtcga tcgggcaacg ttggctgttc aggacatttt tcaggggact aaccccttaa
     1921 ttaaaggctg gattaacgaa gagaagattc agaaaaagat tgacgaacgg atgggcaaca
     1981 cggtcctggg tggcatggcg aaagcgattg tgcataaaat ggccaagaac gaatttcagt
     2041 gcatggcgaa catggatatg ctgggcaatt gcgagaaaca ctgtcagacg agcggcgaaa
     2101 aaggctactg tcacggcacc aagtgtaaat gcggcacccc cctgtcttat tcctgcaggg
     2161 gtggtggcgg ttccggcggc ggtggcagcg gcggtggcgg ttcgccctgc aggatgcctg
     2221 ttctggaaaa ccgggctgct cagggcgata ttactgcacc cggcggtgct cgccgtttaa
     2281 cgggtgatca gactgccgct ctgcgtgatt ctcttagcga taaacctgca aaaaatatta
     2341 ttttgctgat tggcgatggg atgggggact cggaaattac tgccgcacgt aattatgccg
     2401 aaggtgcggg cggctttttt aaaggtatag atgccttacc gcttaccggg caatacactc
     2461 actatgcgct gaataaaaaa accggcaaac cggactacgt caccgactcg gctgcatcag
     2521 caaccgcctg gtcaaccggt gtcaaaacct ataacggcgc gctgggcgtc gatattcacg
     2581 aaaaagatca cccaacgatt ctggaaatgg caaaagccgc aggtctggcg accggtaacg
     2641 tttctaccgc agagttgcag gatgccacgc ccgctgcgct ggtggcacat gtgacctcgc
     2701 gcaaatgcta cggtccgagc gcgaccagtg aaaaatgtcc gggtaacgct ctggaaaaag
     2761 gcggaaaagg atcgattacc gaacagctgc ttaacgctcg tgccgacgtt acgcttggcg
     2821 gcggcgcaaa aacctttgct gaaacggcaa ccgctggtga atggcaggga aaaacgctgc
     2881 gtgaacaggc acaggcgcgt ggttatcagt tggtgagcga tgctgcctca ctgaattcgg
     2941 tgacggaagc gaatcagcaa aaacccctgc ttggcctgtt tgctgacggc aatatgccag
     3001 tgcgctggct aggaccgaaa gcaacgtacc atggcaatat cgataagccc gcagtcacct
     3061 gtacgccaaa tccgcaacgt aatgacagtg taccaaccct ggcgcagatg accgacaaag
     3121 ccattgaatt gttgagtaaa aatgagaaag gctttttcct gcaagttgaa ggtgcgtcaa
     3181 tcgataaaca ggatcatgct gcgaatcctt gtgggcaaat tggcgagacg gtcgatctcg
     3241 atgaagccgt acaacgggcg ctggaattcg ctaaaaagga gggtaacacg ctggtcatag
     3301 tcaccgctga tcacgcccac gccagccaga ttgttgcgcc ggataccaaa gctccgggcc
     3361 tcacccaggc gctaaatacc aaagatggcg cagtgatggt gatgagttac gggaactccg
     3421 aagaggattc acaagaacat accggcagtc agttgcgtat tgcggcgtat ggcccgcatg
     3481 ccgccaatgt tgttggactg accgaccaga ccgatctctt ctacaccatg aaagccgctc
     3541 tggggctgaa ataaaaccgc gcccggcagt gaattttcgc tgccgggtgg tttttttgct
     3601 gttagcaacc agacttaatg gcagatcacg ggcgcatacg ctcatggtta aaacatgaag
     3661 agggatggtg ctatgaaaat aacattactg gttaccttgc ttttcggtct ggttttttta
     3721 accaccgtcg gcgctgccga gagaacttta accccacaac aacagcgtat gacctcctgt
     3781 aatcagcagg cgacggcgca ggcgttgaaa ggggatgctc gtaagaccta catgagtgat
     3841 tgcctgaaga acagcaagtc tgcgcctggc gaaaaaagtt tgacgccaca gcagcaaaag
     3901 atgcgcgaat gcaataatca agcaacacaa caatctctga aaggtgatga tcgtaataag
     3961 tttatgagtg cctgcctcaa gaaagccgcc tgatacctga tagtgctaac gggtgagcta
     4021 cgaaaatggc tcacccgaaa tatcatactt ctgcctttag ctccgtctct ataatttggg
     4081 aaaattgttt ctgaatgttc ccaaaaataa tgaatgatga aaactttttc aaaaaagcgg
     4141 cggcgcacgg ggaggaacct cctttaactc ctcaaaacga acatcagcgg tccgggctgc
     4201 gcttcgcccg tcgcgtcaga ctaccccgtg cggttggcct ggctggcatg ttcttaccga
     4261 ttgcttcaac gctggtttca cacccgccgc cgggctggtg gtggctggtg ttggtcggct
     4321 gggcgttcgt ctggccgcat ttagcctggc agatagcgag cagggccgtc gatccgctta
     4381 gccgggaaat ttacaactta aaaaccgatg cagtattagc gggaatgtgg gtaggcgtaa
     4441 tgggcgtaaa cgtgctgcct tccaccgcga tgttgatgat tatgtgtctg aatttgatgg
     4501 gggcaggcgg cccccgtctg tttgtcgcgg gtctggtgtt gatggtggtt tcctgccttg
     4561 tcaccggccg gccggatccg aattcgcgtt aatattttgt taaaattcgc gttaaatttt
     4621 tgttaaatca gctcattttt taaccaatag gccgactgcg atgagtggca gggcggggcg
     4681 taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg
     4741 ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg
     4801 gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg
     4861 aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt
     4921 ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga taaaaacggt
     4981 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     5041 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgaggcggct acagccgata
     5101 gtctggaaca gcgcacttac gggttgctgc gcaacccaag tgctaccggc gcggcagcgt
     5161 gacccgtgtc ggcggctcca acggctcgcc atcgtccaga aaacacggct catcgggcat
     5221 cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc
     5281 tggttccatg cccggaatgc cgggctggct gggcggctcc tcgccggggc cggtcggtag
     5341 ttgctgctcg cccggataca gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga
     5401 ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg aacaccgaca ggcgcaactg
     5461 gtcgcggggc tggccccacg ccacgcggtc attgaccacg taggccgaca cggtgccggg
     5521 gccgttgagc ttcacgacgg agatccagcg ctcggccacc aagtccttga ctgcgtattg
     5581 gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga ccaccacggc
     5641 gttctggtgg cccatctgcg ccacgaggtg atgcagcagc attgccgccg tgggtttcct
     5701 cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg
     5761 cttgttcttg gcttgaatgc cgatttctct ggactgcgtg gccatgctta tctccatgcg
     5821 gtagggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga
     5881 caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa
     5941 tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg
     6001 atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac
     6061 ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc
     6121 cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg
     6181 gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgccttcgac
     6241 tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg
     6301 tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca
     6361 ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc
     6421 aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc
     6481 cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc
     6541 cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac
     6601 gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg
     6661 cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga gtatactcaa
     6721 gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac
     6781 caaaagggag gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa
     6841 atgggcaacg tggcggccag tctcaagcac gcctaccgcg agcgcgagac gcccaacgct
     6901 gacgccagca ggacgccaga gaacgagcac tgggcggcca gcagcaccga tgaagcgatg
     6961 ggccgactgc gcgagttgct gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag
     7021 tacgtcatga cggccagccc ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg
     7081 ttcttcgaga aggcgcacaa gtggctggcg gacaagtacg gggcggatcg catcgtgacg
     7141 gccagcatcc accgtgacga aaccagcccg cacatgaccg cgttcgtggt gccgctgacg
     7201 caggacggca ggctgtcggc caaggagttc atcggcaaca aagcgcagat gacccgcgac
     7261 cagaccacgt ttgcggccgc tgtggccgat ctagggctgc aacggggcat cgagggcagc
     7321 aaggcacgtc acacgcgcat tcaggcgttc tacgaggccc tggagcggcc accagtgggc
     7381 cacgtcacca tcagcccgca agcggtcgag ccacgcgcct atgcaccgca gggattggcc
     7441 gaaaagctgg gaatctcaaa gcgcgttgag acgccggaag ccgtggccga ccggctgaca
     7501 aaagcggttc ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg
     7561 cgcaagaagg ccgatcaagc ccaagagacg gcccgag
//
