LOCUS       V015930                 7315 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V015930
VERSION     V015930
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1913
                     /label="hypothetical protein 2 secretion signal"
                     /note="hypothetical protein 2 secretion signal"
     CDS             1920..3269
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4864..5523)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5524..6293)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgc gctcatttac ccgccgtgcc cttgctgctt
     1801 cgacctttgc cgctgcggct ctggccgtca tgccggtagc gcatgcagcc cccgatgcac
     1861 tgggcacccc cgttggccac atcaccctga tggcgaaatc cgccgatgtg ggtttaatta
     1921 aaacctgcag gatgcctgtt ctggaaaacc gggctgctca gggcgatatt actgcacccg
     1981 gcggtgctcg ccgtttaacg ggtgatcaga ctgccgctct gcgtgattct cttagcgata
     2041 aacctgcaaa aaatattatt ttgctgattg gcgatgggat gggggactcg gaaattactg
     2101 ccgcacgtaa ttatgccgaa ggtgcgggcg gcttttttaa aggtatagat gccttaccgc
     2161 ttaccgggca atacactcac tatgcgctga ataaaaaaac cggcaaaccg gactacgtca
     2221 ccgactcggc tgcatcagca accgcctggt caaccggtgt caaaacctat aacggcgcgc
     2281 tgggcgtcga tattcacgaa aaagatcacc caacgattct ggaaatggca aaagccgcag
     2341 gtctggcgac cggtaacgtt tctaccgcag agttgcagga tgccacgccc gctgcgctgg
     2401 tggcacatgt gacctcgcgc aaatgctacg gtccgagcgc gaccagtgaa aaatgtccgg
     2461 gtaacgctct ggaaaaaggc ggaaaaggat cgattaccga acagctgctt aacgctcgtg
     2521 ccgacgttac gcttggcggc ggcgcaaaaa cctttgctga aacggcaacc gctggtgaat
     2581 ggcagggaaa aacgctgcgt gaacaggcac aggcgcgtgg ttatcagttg gtgagcgatg
     2641 ctgcctcact gaattcggtg acggaagcga atcagcaaaa acccctgctt ggcctgtttg
     2701 ctgacggcaa tatgccagtg cgctggctag gaccgaaagc aacgtaccat ggcaatatcg
     2761 ataagcccgc agtcacctgt acgccaaatc cgcaacgtaa tgacagtgta ccaaccctgg
     2821 cgcagatgac cgacaaagcc attgaattgt tgagtaaaaa tgagaaaggc tttttcctgc
     2881 aagttgaagg tgcgtcaatc gataaacagg atcatgctgc gaatccttgt gggcaaattg
     2941 gcgagacggt cgatctcgat gaagccgtac aacgggcgct ggaattcgct aaaaaggagg
     3001 gtaacacgct ggtcatagtc accgctgatc acgcccacgc cagccagatt gttgcgccgg
     3061 ataccaaagc tccgggcctc acccaggcgc taaataccaa agatggcgca gtgatggtga
     3121 tgagttacgg gaactccgaa gaggattcac aagaacatac cggcagtcag ttgcgtattg
     3181 cggcgtatgg cccgcatgcc gccaatgttg ttggactgac cgaccagacc gatctcttct
     3241 acaccatgaa agccgctctg gggctgaaat aaaaccgcgc ccggcagtga attttcgctg
     3301 ccgggtggtt tttttgctgt tagcaaccag acttaatggc agatcacggg cgcatacgct
     3361 catggttaaa acatgaagag ggatggtgct atgaaaataa cattactggt taccttgctt
     3421 ttcggtctgg tttttttaac caccgtcggc gctgccgaga gaactttaac cccacaacaa
     3481 cagcgtatga cctcctgtaa tcagcaggcg acggcgcagg cgttgaaagg ggatgctcgt
     3541 aagacctaca tgagtgattg cctgaagaac agcaagtctg cgcctggcga aaaaagtttg
     3601 acgccacagc agcaaaagat gcgcgaatgc aataatcaag caacacaaca atctctgaaa
     3661 ggtgatgatc gtaataagtt tatgagtgcc tgcctcaaga aagccgcctg atacctgata
     3721 gtgctaacgg gtgagctacg aaaatggctc acccgaaata tcatacttct gcctttagct
     3781 ccgtctctat aatttgggaa aattgtttct gaatgttccc aaaaataatg aatgatgaaa
     3841 actttttcaa aaaagcggcg gcgcacgggg aggaacctcc tttaactcct caaaacgaac
     3901 atcagcggtc cgggctgcgc ttcgcccgtc gcgtcagact accccgtgcg gttggcctgg
     3961 ctggcatgtt cttaccgatt gcttcaacgc tggtttcaca cccgccgccg ggctggtggt
     4021 ggctggtgtt ggtcggctgg gcgttcgtct ggccgcattt agcctggcag atagcgagca
     4081 gggccgtcga tccgcttagc cgggaaattt acaacttaaa aaccgatgca gtattagcgg
     4141 gaatgtgggt aggcgtaatg ggcgtaaacg tgctgccttc caccgcgatg ttgatgatta
     4201 tgtgtctgaa tttgatgggg gcaggcggcc cccgtctgtt tgtcgcgggt ctggtgttga
     4261 tggtggtttc ctgccttgtc accggccggc cggatccgaa ttcgcgttaa tattttgtta
     4321 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgactgcgat
     4381 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
     4441 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
     4501 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
     4561 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
     4621 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
     4681 gagcctgata aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     4741 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     4801 aggcggctac agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg
     4861 ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
     4921 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
     4981 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
     5041 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
     5101 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
     5161 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
     5221 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
     5281 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
     5341 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
     5401 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
     5461 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
     5521 catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc
     5581 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     5641 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     5701 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     5761 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     5821 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     5881 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     5941 gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     6001 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     6061 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     6121 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     6181 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     6241 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     6301 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     6361 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     6421 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     6481 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     6541 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     6601 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     6661 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     6721 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     6781 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     6841 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     6901 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     6961 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     7021 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     7081 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     7141 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     7201 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     7261 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgag
//
