LOCUS       62056_12630        7591 bp DNA     circular SYN 24-FEB-2021
DEFINITION  Cloning vector pHyp1s kanamycin resistance protein gene, complete 
            cds.
ACCESSION   MW132121
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition 
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University, 
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes 
            Ave, Pittsburgh, PA 15219-3016, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label=PnptII
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1910
                     /label=hypothetical protein 1 secretion signal
                     /note="hypothetical protein 1 secretion signal"
     misc_feature    1920..2144
                     /label=scorpine
                     /note="scorpine"
     misc_feature    2211..3548
                     /label=alkaline phosphatase, no leader signal
                     /note="alkaline phosphatase, no leader signal"
     CDS             complement(5140..5799)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5800..6569)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgc tcgttttcaa tcgtcgtctt cttaccggtg
     1801 ttgccgctct tgccatgacg ctgagcgccc ctgctttcgc tcagaccacg caggaagcag
     1861 ccccggcagc ccctgctgct gcagctcccg ctgccccggc agccgcgcct ttaattaaag
     1921 gctggattaa cgaagagaag attcagaaaa agattgacga acggatgggc aacacggtcc
     1981 tgggtggcat ggcgaaagcg attgtgcata aaatggccaa gaacgaattt cagtgcatgg
     2041 cgaacatgga tatgctgggc aattgcgaga aacactgtca gacgagcggc gaaaaaggct
     2101 actgtcacgg caccaagtgt aaatgcggca cccccctgtc ttattcctgc aggggtggtg
     2161 gcggttccgg cggcggtggc agcggcggtg gcggttcgcc ctgcaggatg cctgttctgg
     2221 aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt ttaacgggtg
     2281 atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat attattttgc
     2341 tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat gccgaaggtg
     2401 cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac actcactatg
     2461 cgctgaataa aaaaaccggc aaaccggact acgtcaccga ctcggctgca tcagcaaccg
     2521 cctggtcaac cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt cacgaaaaag
     2581 atcacccaac gattctggaa atggcaaaag ccgcaggtct ggcgaccggt aacgtttcta
     2641 ccgcagagtt gcaggatgcc acgcccgctg cgctggtggc acatgtgacc tcgcgcaaat
     2701 gctacggtcc gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa aaaggcggaa
     2761 aaggatcgat taccgaacag ctgcttaacg ctcgtgccga cgttacgctt ggcggcggcg
     2821 caaaaacctt tgctgaaacg gcaaccgctg gtgaatggca gggaaaaacg ctgcgtgaac
     2881 aggcacaggc gcgtggttat cagttggtga gcgatgctgc ctcactgaat tcggtgacgg
     2941 aagcgaatca gcaaaaaccc ctgcttggcc tgtttgctga cggcaatatg ccagtgcgct
     3001 ggctaggacc gaaagcaacg taccatggca atatcgataa gcccgcagtc acctgtacgc
     3061 caaatccgca acgtaatgac agtgtaccaa ccctggcgca gatgaccgac aaagccattg
     3121 aattgttgag taaaaatgag aaaggctttt tcctgcaagt tgaaggtgcg tcaatcgata
     3181 aacaggatca tgctgcgaat ccttgtgggc aaattggcga gacggtcgat ctcgatgaag
     3241 ccgtacaacg ggcgctggaa ttcgctaaaa aggagggtaa cacgctggtc atagtcaccg
     3301 ctgatcacgc ccacgccagc cagattgttg cgccggatac caaagctccg ggcctcaccc
     3361 aggcgctaaa taccaaagat ggcgcagtga tggtgatgag ttacgggaac tccgaagagg
     3421 attcacaaga acataccggc agtcagttgc gtattgcggc gtatggcccg catgccgcca
     3481 atgttgttgg actgaccgac cagaccgatc tcttctacac catgaaagcc gctctggggc
     3541 tgaaataaaa ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt tgctgttagc
     3601 aaccagactt aatggcagat cacgggcgca tacgctcatg gttaaaacat gaagagggat
     3661 ggtgctatga aaataacatt actggttacc ttgcttttcg gtctggtttt tttaaccacc
     3721 gtcggcgctg ccgagagaac tttaacccca caacaacagc gtatgacctc ctgtaatcag
     3781 caggcgacgg cgcaggcgtt gaaaggggat gctcgtaaga cctacatgag tgattgcctg
     3841 aagaacagca agtctgcgcc tggcgaaaaa agtttgacgc cacagcagca aaagatgcgc
     3901 gaatgcaata atcaagcaac acaacaatct ctgaaaggtg atgatcgtaa taagtttatg
     3961 agtgcctgcc tcaagaaagc cgcctgatac ctgatagtgc taacgggtga gctacgaaaa
     4021 tggctcaccc gaaatatcat acttctgcct ttagctccgt ctctataatt tgggaaaatt
     4081 gtttctgaat gttcccaaaa ataatgaatg atgaaaactt tttcaaaaaa gcggcggcgc
     4141 acggggagga acctccttta actcctcaaa acgaacatca gcggtccggg ctgcgcttcg
     4201 cccgtcgcgt cagactaccc cgtgcggttg gcctggctgg catgttctta ccgattgctt
     4261 caacgctggt ttcacacccg ccgccgggct ggtggtggct ggtgttggtc ggctgggcgt
     4321 tcgtctggcc gcatttagcc tggcagatag cgagcagggc cgtcgatccg cttagccggg
     4381 aaatttacaa cttaaaaacc gatgcagtat tagcgggaat gtgggtaggc gtaatgggcg
     4441 taaacgtgct gccttccacc gcgatgttga tgattatgtg tctgaatttg atgggggcag
     4501 gcggcccccg tctgtttgtc gcgggtctgg tgttgatggt ggtttcctgc cttgtcaccg
     4561 gccggccgga tccgaattcg cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4621 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
     4681 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     4741 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     4801 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     4861 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     4921 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     4981 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     5041 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     5101 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     5161 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     5221 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     5281 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     5341 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     5401 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     5461 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     5521 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     5581 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     5641 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     5701 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     5761 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     5821 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
     5881 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
     5941 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
     6001 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
     6061 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
     6121 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
     6181 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
     6241 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     6301 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     6361 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     6421 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     6481 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     6541 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     6601 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
     6661 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
     6721 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
     6781 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
     6841 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
     6901 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
     6961 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
     7021 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
     7081 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
     7141 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
     7201 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
     7261 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
     7321 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
     7381 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
     7441 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
     7501 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
     7561 aaggccgatc aagcccaaga gacggcccga g
//
