LOCUS       V015894                 7312 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V015894
VERSION     V015894
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7312)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1910
                     /label="hypothetical protein 1 secretion signal"
                     /note="hypothetical protein 1 secretion signal"
     CDS             1917..3266
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4861..5520)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5521..6290)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgc tcgttttcaa tcgtcgtctt cttaccggtg
     1801 ttgccgctct tgccatgacg ctgagcgccc ctgctttcgc tcagaccacg caggaagcag
     1861 ccccggcagc ccctgctgct gcagctcccg ctgccccggc agccgcgcct ttaattaaaa
     1921 cctgcaggat gcctgttctg gaaaaccggg ctgctcaggg cgatattact gcacccggcg
     1981 gtgctcgccg tttaacgggt gatcagactg ccgctctgcg tgattctctt agcgataaac
     2041 ctgcaaaaaa tattattttg ctgattggcg atgggatggg ggactcggaa attactgccg
     2101 cacgtaatta tgccgaaggt gcgggcggct tttttaaagg tatagatgcc ttaccgctta
     2161 ccgggcaata cactcactat gcgctgaata aaaaaaccgg caaaccggac tacgtcaccg
     2221 actcggctgc atcagcaacc gcctggtcaa ccggtgtcaa aacctataac ggcgcgctgg
     2281 gcgtcgatat tcacgaaaaa gatcacccaa cgattctgga aatggcaaaa gccgcaggtc
     2341 tggcgaccgg taacgtttct accgcagagt tgcaggatgc cacgcccgct gcgctggtgg
     2401 cacatgtgac ctcgcgcaaa tgctacggtc cgagcgcgac cagtgaaaaa tgtccgggta
     2461 acgctctgga aaaaggcgga aaaggatcga ttaccgaaca gctgcttaac gctcgtgccg
     2521 acgttacgct tggcggcggc gcaaaaacct ttgctgaaac ggcaaccgct ggtgaatggc
     2581 agggaaaaac gctgcgtgaa caggcacagg cgcgtggtta tcagttggtg agcgatgctg
     2641 cctcactgaa ttcggtgacg gaagcgaatc agcaaaaacc cctgcttggc ctgtttgctg
     2701 acggcaatat gccagtgcgc tggctaggac cgaaagcaac gtaccatggc aatatcgata
     2761 agcccgcagt cacctgtacg ccaaatccgc aacgtaatga cagtgtacca accctggcgc
     2821 agatgaccga caaagccatt gaattgttga gtaaaaatga gaaaggcttt ttcctgcaag
     2881 ttgaaggtgc gtcaatcgat aaacaggatc atgctgcgaa tccttgtggg caaattggcg
     2941 agacggtcga tctcgatgaa gccgtacaac gggcgctgga attcgctaaa aaggagggta
     3001 acacgctggt catagtcacc gctgatcacg cccacgccag ccagattgtt gcgccggata
     3061 ccaaagctcc gggcctcacc caggcgctaa ataccaaaga tggcgcagtg atggtgatga
     3121 gttacgggaa ctccgaagag gattcacaag aacataccgg cagtcagttg cgtattgcgg
     3181 cgtatggccc gcatgccgcc aatgttgttg gactgaccga ccagaccgat ctcttctaca
     3241 ccatgaaagc cgctctgggg ctgaaataaa accgcgcccg gcagtgaatt ttcgctgccg
     3301 ggtggttttt ttgctgttag caaccagact taatggcaga tcacgggcgc atacgctcat
     3361 ggttaaaaca tgaagaggga tggtgctatg aaaataacat tactggttac cttgcttttc
     3421 ggtctggttt ttttaaccac cgtcggcgct gccgagagaa ctttaacccc acaacaacag
     3481 cgtatgacct cctgtaatca gcaggcgacg gcgcaggcgt tgaaagggga tgctcgtaag
     3541 acctacatga gtgattgcct gaagaacagc aagtctgcgc ctggcgaaaa aagtttgacg
     3601 ccacagcagc aaaagatgcg cgaatgcaat aatcaagcaa cacaacaatc tctgaaaggt
     3661 gatgatcgta ataagtttat gagtgcctgc ctcaagaaag ccgcctgata cctgatagtg
     3721 ctaacgggtg agctacgaaa atggctcacc cgaaatatca tacttctgcc tttagctccg
     3781 tctctataat ttgggaaaat tgtttctgaa tgttcccaaa aataatgaat gatgaaaact
     3841 ttttcaaaaa agcggcggcg cacggggagg aacctccttt aactcctcaa aacgaacatc
     3901 agcggtccgg gctgcgcttc gcccgtcgcg tcagactacc ccgtgcggtt ggcctggctg
     3961 gcatgttctt accgattgct tcaacgctgg tttcacaccc gccgccgggc tggtggtggc
     4021 tggtgttggt cggctgggcg ttcgtctggc cgcatttagc ctggcagata gcgagcaggg
     4081 ccgtcgatcc gcttagccgg gaaatttaca acttaaaaac cgatgcagta ttagcgggaa
     4141 tgtgggtagg cgtaatgggc gtaaacgtgc tgccttccac cgcgatgttg atgattatgt
     4201 gtctgaattt gatgggggca ggcggccccc gtctgtttgt cgcgggtctg gtgttgatgg
     4261 tggtttcctg ccttgtcacc ggccggccgg atccgaattc gcgttaatat tttgttaaaa
     4321 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga ctgcgatgag
     4381 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggtgct
     4441 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgaaagca aattcgaccc
     4501 ggtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct ggtttaccgg
     4561 tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc
     4621 tcaggctctc cccgtggagg taataattga cgatatgatc atttattctg cctcccagag
     4681 cctgataaaa acggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
     4741 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgagg
     4801 cggctacagc cgatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta
     4861 ccggcgcggc agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca
     4921 cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt
     4981 caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc
     5041 ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc
     5101 atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac
     5161 cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc
     5221 cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc
     5281 cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg
     5341 gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc
     5401 cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg
     5461 cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat
     5521 gcttatctcc atgcggtagg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac
     5581 ttttcggcag cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt
     5641 ctttaaccta cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc
     5701 ttttttaagt tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg
     5761 cccaaagaag ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc
     5821 aaaaagtggc ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt
     5881 ggcgctgccc ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg
     5941 ggcttcgcct tcgactgccc ccactcgcat aggcttgggt cgttccaggc gcgtcaaggc
     6001 caagccgctg cgcggtcgct gcgcgagcct tgacccgcct tccacttggt gtccaaccgg
     6061 caagcgaagc gcgcaggccg caggccggag gcttttcccc agagaaaatt aaaaaaattg
     6121 atggggcaag gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg aaggctgggt
     6181 agccggtggg caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc catcagcttg
     6241 tccagcaggg ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc tcgcggccat
     6301 cgtccacata tccacgggct ggcaagggag cgcagcgacc gcgcagggcg aagcccggag
     6361 agcaagcccg tagggcgccg cagccgccgt aggcggtcac gactttgcga agcaaagtct
     6421 agtgagtata ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc tgcccccgtc
     6481 gagccggttg gacaccaaaa gggaggggca ggcatggcgg catacgcgat catgcgatgc
     6541 aagaagctgg cgaaaatggg caacgtggcg gccagtctca agcacgccta ccgcgagcgc
     6601 gagacgccca acgctgacgc cagcaggacg ccagagaacg agcactgggc ggccagcagc
     6661 accgatgaag cgatgggccg actgcgcgag ttgctgccag agaagcggcg caaggacgct
     6721 gtgttggcgg tcgagtacgt catgacggcc agcccggaat ggtggaagtc ggccagccaa
     6781 gaacagcagg cggcgttctt cgagaaggcg cacaagtggc tggcggacaa gtacggggcg
     6841 gatcgcatcg tgacggccag catccaccgt gacgaaacca gcccgcacat gaccgcgttc
     6901 gtggtgccgc tgacgcagga cggcaggctg tcggccaagg agttcatcgg caacaaagcg
     6961 cagatgaccc gcgaccagac cacgtttgcg gccgctgtgg ccgatctagg gctgcaacgg
     7021 ggcatcgagg gcagcaaggc acgtcacacg cgcattcagg cgttctacga ggccctggag
     7081 cggccaccag tgggccacgt caccatcagc ccgcaagcgg tcgagccacg cgcctatgca
     7141 ccgcagggat tggccgaaaa gctgggaatc tcaaagcgcg ttgagacgcc ggaagccgtg
     7201 gccgaccggc tgacaaaagc ggttcggcag gggtatgagc ctgccctaca ggccgccgca
     7261 ggagcgcgtg agatgcgcaa gaaggccgat caagcccaag agacggcccg ag
//
