LOCUS       40924_25261        6257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Dana CE, Glauber KM, Chan TA, Bridge DM, Steele RE.
  TITLE     Incorporation of a horizontally transferred gene into an operon 
            during cnidarian evolution
  JOURNAL   PLoS ONE 7 (2), E31643 (2012)
  PUBMED    22328943
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Steele RE, Bridge DM, Chan TA, Dana K.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6257)
  AUTHORS   Steele RE, Bridge DM, Chan TA, Dana K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2007) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   4  (bases 1 to 6257)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "2"; pages: "E31643"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2007) Department of Biological Chemistry, University of 
            California, Irvine, D240 Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /label=Hydra vulgaris actin
                     /note="Hydra vulgaris actin"
                     /regulatory_class="promoter"
     CDS             1705..2418
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     misc_feature    2428..2863
                     /note="intergenic region between Hydra replication factor C
                     large subunit gene and Hydra flp gene"
     misc_feature    2844..2845
                     /note="splice acceptor dinucleotide for trans-spliced
                     leader"
     CDS             2882..3556
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    3560..4055
                     /note="3' flanking sequence from Hydra vulgaris actin gene"
     regulatory      3691..3696
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(4069..4087)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4108..4124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4132..4148)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4156..4186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4201..4222)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4510..5098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5272..6129)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6130..6234)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggttttaag
      661 ccgagatgca ggattctgaa tgaaaaagaa aaaaagaagt ctcggtagag taaaagtgat
      721 cggtttgcaa ctgtaaaatt tattgaagta ccaataattt tatttaaaat aaaactgaaa
      781 tataaagtta aagttgctgt tctataagtt taccgaattt taaaaccatt gtaacgctag
      841 agtaatattt gagtctacta agttagtccc cgcacttttt aatcaagcaa taaataccca
      901 aactttgctt attcaaatca ataaaccaat atatctctta aaataaagta aaaacttctg
      961 aaattctata aaaaaaaatt taatttcgaa atatcaaatg taacttcaac accgcactat
     1021 tttcttttaa acaactgata tagtaattac ttctcaaaaa cgttatctca aggtttgtga
     1081 tgtacttaaa accactccta ttttgttacg cgtttaaaaa agcaaacata agttggtttc
     1141 tattgatgaa tgagaacata tttcatttaa agttaaaatc ctaccagtgg tttcactgta
     1201 cgtaaacacc gtcaaaaaaa caggaacgtt tttaaagatt aataattgaa gtaaaaaaaa
     1261 tttaataccg ggggttaaaa aaatctttta aaataattat aaatatatat attaaaattt
     1321 ataaattttt aaacacattt aaaatatata ttaagtataa taaaagtaat attataaaaa
     1381 aaaatttaat tttataatta tttttattaa atttataaat aataggtaaa acttacatat
     1441 ccgttttatt ttttcttaat aaaataacgc gtgcaaattt ttgtccatat aaagaccttt
     1501 tcgaacaata acttttttgc ttagccgttt tttttcttat atggtcaaaa aagcgctcaa
     1561 gcgattcacc ataaaaagcg caattagttc agcgttcgtt attcagaagc ttcagctttg
     1621 cttgatactc agctcttctc tttttaaaca aaacacttaa tcaaaatggc cgatgatgaa
     1681 gttgccgccc tcgctgcagg atccatgagt aaaggagaag aacttttcac tggagttgtc
     1741 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     1801 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     1861 ctacctgttc catggccaac acttgtcact actttctgtt atggtgttca atgcttttca
     1921 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     1981 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     2041 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     2101 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     2161 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     2221 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     2281 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     2341 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     2401 atggatgaac tatacaaaga tccataaatc tgattgcgat cttttgtttt ccaataaaac
     2461 aaagataccg aaagttaccg atttttctta ccgaaagttt gacaatgctt tgttgttctc
     2521 catttttcta ttgtaattaa aaacattttt tcttttttta agaagttgtg ctttttataa
     2581 atttgtgtaa aaacgcgtta taatgctaag ttgagggtta aagtttactg acctgataat
     2641 atatcgagcg aggattatta tataattaaa gatttattag tatatatagt atataacatg
     2701 taaactgata gtatatactt gatatattaa tgattaatat atcaagcgag gattcattta
     2761 tcattaaagt atttgcgatc gtcgcttgta acattaaatt ttaaatttta ccttattaaa
     2821 acttcataat tttttttatt tttagatagt aaatatagta gcaatggcag atgctgcaga
     2881 aatggcttca tcagaaaatg ttattacaga atttatgaga tttaaagtta gaatggaagg
     2941 tacagttaat ggacatgaat ttgaaattga gggagaagga gaaggtagac catatgaagg
     3001 acataataca gttaaattaa aggttacaaa aggaggacca ttaccatttg cgtgggatat
     3061 tttatcacca caatttcaat atggatcaaa ggtttacgtt aaacatccag ctgatattcc
     3121 agattataaa aaattatctt ttccagaagg atttaaatgg gaaagagtta tgaattttga
     3181 agatggtgga gttgctacag ttacacaaga ttcatcatta caagatggtt gttttattta
     3241 taaagttaaa tttattgggg ttaattttcc atcagacgga ccagttatgc aaaaaaaaac
     3301 aatgggatgg gaagctagta cagaaagatt atatccaaga gacggggttt taaagggaga
     3361 aacacataaa gctctaaaat taaaggacgg aggacattat ttagttgaat ttaaatcaat
     3421 ttatatggct aaaaaaccag ttcaattacc aggatattat tatgtagatg ctaagttaga
     3481 tattacatca cataatgaag attatacaat tgttgaacaa tatgaaagaa cagaaggtag
     3541 acatcattta tttttatagc attcgtagaa ttcacaattc gattatattt atactggact
     3601 atttttacat ctgttcggtt attttcacat ttatttttct atatatatct tataaacgtt
     3661 ttaaaaccca tgtaattttt gttaagctgt aatataaaag acgtcctaac aaacttcttt
     3721 tattactgaa tttcctttaa ttataataaa taacaagttt taaaataaat tcaggcaatt
     3781 aaggcgctcc tgaggtacta aaattaatgt aaacatttaa aattaacttg gatggtctta
     3841 agtactgtac tcgtgatttt gttatacttt attattagaa aagtcgtcta ttaacttttt
     3901 gttccttaat ttacttgatt aaattgtcgc ttaatttatc aaatcaggtt ttgcgcgtta
     3961 ttttagagaa aaacttatta gaaaaatgaa taagcaaagt ttaggctaac atgttttttt
     4021 attattttaa atagttcaag tcaatgacgt ataaaccagc ttttgttccc tttagtgagg
     4081 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4921 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6241 tccccgaaaa gtgccac
//
