LOCUS       40924_25246        5112 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2004) Dept. of Biological Chemistry, University of
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA
REFERENCE   3  (bases 1 to 5112)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-APR-2004) Dept. of Biological Chemistry, University of 
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /label=Hydra vulgaris actin promoter
                     /note="Hydra vulgaris actin promoter"
                     /regulatory_class="promoter"
     misc_feature    1666..1668
                     /label=Hydra vulgaris actin start codon
                     /note="Hydra vulgaris actin start codon"
     CDS             1698..2411
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     misc_feature    2415..2910
                     /label=3' flanking sequence from Hydra vulgaris actin
                     /note="3' flanking sequence from Hydra vulgaris actin"
     regulatory      2546..2551
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(2924..2942)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2963..2979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2987..3003)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3011..3041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3056..3077)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3365..3953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4127..4984)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4985..5089)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggttttaag
      661 ccgagatgca ggattctgaa tgaaaaagaa aaaaagaagt ctcggtagag taaaagtgat
      721 cggtttgcaa ctgtaaaatt tattgaagta ccaataattt tatttaaaat aaaactgaaa
      781 tataaagtta aagttgctgt tctataagtt taccgaattt taaaaccatt gtaacgctag
      841 agtaatattt gagtctacta agttagtccc cgcacttttt aatcaagcaa taaataccca
      901 aactttgctt attcaaatca ataaaccaat atatctctta aaataaagta aaaacttctg
      961 aaattctata aaaaaaaatt taatttcgaa atatcaaatg taacttcaac accgcactat
     1021 tttcttttaa acaactgata tagtaattac ttctcaaaaa cgttatctca aggtttgtga
     1081 tgtacttaaa accactccta ttttgttacg cgtttaaaaa agcaaacata agttggtttc
     1141 tattgatgaa tgagaacata tttcatttaa agttaaaatc ctaccagtgg tttcactgta
     1201 cgtaaacacc gtcaaaaaaa caggaacgtt tttaaagatt aataattgaa gtaaaaaaaa
     1261 tttaataccg ggggttaaaa aaatctttta aaataattat aaatatatat attaaaattt
     1321 ataaattttt aaacacattt aaaatatata ttaagtataa taaaagtaat attataaaaa
     1381 aaaatttaat tttataatta tttttattaa atttataaat aataggtaaa acttacatat
     1441 ccgttttatt ttttcttaat aaaataacgc gtgcaaattt ttgtccatat aaagaccttt
     1501 tcgaacaata acttttttgc ttagccgttt tttttcttat atggtcaaaa aagcgctcaa
     1561 gcgattcacc ataaaaagcg caattagttc agcgttcgtt attcagaagc ttcagctttg
     1621 cttgatactc agctcttctc tttttaaaca aaacacttaa tcaaaatgca tgggatactg
     1681 cagccccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     1741 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1801 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1861 ttccatggcc aacacttgtc actactttct gttatggtgt tcaatgcttt tcaagatacc
     1921 cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1981 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     2041 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     2101 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     2161 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     2221 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     2281 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     2341 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     2401 aactatacaa atagcattcg tagaattcac aattcgatta tatttatact ggactatttt
     2461 tacatctgtt cggttatttt cacatttatt tttctatata tatcttataa acgttttaaa
     2521 acccatgtaa tttttgttaa gctgtaatat aaaagacgtc ctaacaaact tcttttatta
     2581 ctgaatttcc tttaattata ataaataaca agttttaaaa taaattcagg caattaaggc
     2641 gctcctgagg tactaaaatt aatgtaaaca tttaaaatta acttggatgg tcttaagtac
     2701 tgtactcgtg attttgttat actttattat tagaaaagtc gtctattaac tttttgttcc
     2761 ttaatttact tgattaaatt gtcgcttaat ttatcaaatc aggttttgcg cgttatttta
     2821 gagaaaaact tattagaaaa atgaataagc aaagtttagg ctaacatgtt tttttattat
     2881 tttaaatagt tcaagtcaat gacgtataaa ccagcttttg ttccctttag tgagggttaa
     2941 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3121 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3781 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5101 gaaaagtgcc ac
//
