LOCUS       40924_25221        5525 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Dana CE, Glauber KM, Chan TA, Bridge DM, Steele RE.
  TITLE     Incorporation of a horizontally transferred gene into an operon 
            during cnidarian evolution
  JOURNAL   PLoS ONE 7 (2), E31643 (2012)
  PUBMED    22328943
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Steele R, Chan T, Dana C.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5525)
  AUTHORS   Steele R, Chan T, Dana C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2007) Department of Biological Chemistry, 
            University of California, Irvine, 240D Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   4  (bases 1 to 5525)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "2"; pages: "E31643"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (29-SEP-2007) Department of Biological Chemistry, University of 
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /label=Hydra vulgaris actin
                     /note="Hydra vulgaris actin"
                     /regulatory_class="promoter"
     misc_feature    1665..1691
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1696..2131
                     /note="intergenic region between Hydra replication factor C
                     large subunit gene and flp gene"
     misc_feature    2112..2113
                     /note="splice acceptor dinucleotide for trans-spliced
                     leader"
     CDS             2150..2824
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    2828..3323
                     /note="3' flanking sequence from Hydra vulgaris actin gene"
     regulatory      2959..2964
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(3337..3355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3400..3416)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3424..3454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3469..3490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3778..4366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4540..5397)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5398..5502)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggttttaag
      661 ccgagatgca ggattctgaa tgaaaaagaa aaaaagaagt ctcggtagag taaaagtgat
      721 cggtttgcaa ctgtaaaatt tattgaagta ccaataattt tatttaaaat aaaactgaaa
      781 tataaagtta aagttgctgt tctataagtt taccgaattt taaaaccatt gtaacgctag
      841 agtaatattt gagtctacta agttagtccc cgcacttttt aatcaagcaa taaataccca
      901 aactttgctt attcaaatca ataaaccaat atatctctta aaataaagta aaaacttctg
      961 aaattctata aaaaaaaatt taatttcgaa atatcaaatg taacttcaac accgcactat
     1021 tttcttttaa acaactgata tagtaattac ttctcaaaaa cgttatctca aggtttgtga
     1081 tgtacttaaa accactccta ttttgttacg cgtttaaaaa agcaaacata agttggtttc
     1141 tattgatgaa tgagaacata tttcatttaa agttaaaatc ctaccagtgg tttcactgta
     1201 cgtaaacacc gtcaaaaaaa caggaacgtt tttaaagatt aataattgaa gtaaaaaaaa
     1261 tttaataccg ggggttaaaa aaatctttta aaataattat aaatatatat attaaaattt
     1321 ataaattttt aaacacattt aaaatatata ttaagtataa taaaagtaat attataaaaa
     1381 aaaatttaat tttataatta tttttattaa atttataaat aataggtaaa acttacatat
     1441 ccgttttatt ttttcttaat aaaataacgc gtgcaaattt ttgtccatat aaagaccttt
     1501 tcgaacaata acttttttgc ttagccgttt tttttcttat atggtcaaaa aagcgctcaa
     1561 gcgattcacc ataaaaagcg caattagttc agcgttcgtt attcagaagc ttcagctttg
     1621 cttgataatc agctcttctc tttttaaaca aaacacttaa tcaagctagc cgatgatgtt
     1681 aacgaggatc cataaatctg attgcgatct tttgttttcc aataaaacaa agataccgaa
     1741 agttaccgat ttttcttacc gaaagtttga caatgctttg ttgttctcca tttttctatt
     1801 gtaattaaaa acattttttc tttttttaag aagttgtgct ttttataaat ttgtgtaaaa
     1861 acgcgttata atgctaagtt gagggttaaa gtttactgac ctgataatat atcgagcgag
     1921 gattattata taattaaaga tttattagta tatatagtat ataacatgta aactgatagt
     1981 atatacttga tatattaatg attaatatat caagcgagga ttcatttatc attaaagtat
     2041 ttgcgatcgt cgcttgtaac attaaatttt aaattttacc ttattaaaac ttcataattt
     2101 tttttatttt tagatagtaa atatagtagc aatggcagat gctgcagaaa tggcttcatc
     2161 agaaaatgtt attacagaat ttatgagatt taaagttaga atggaaggta cagttaatgg
     2221 acatgaattt gaaattgagg gagaaggaga aggtagacca tatgaaggac ataatacagt
     2281 taaattaaag gttacaaaag gaggaccatt accatttgcg tgggatattt tatcaccaca
     2341 atttcaatat ggatcaaagg tttacgttaa acatccagct gatattccag attataaaaa
     2401 attatctttt ccagaaggat ttaaatggga aagagttatg aattttgaag atggtggagt
     2461 tgctacagtt acacaagatt catcattaca agatggttgt tttatttata aagttaaatt
     2521 tattggggtt aattttccat cagacggacc agttatgcaa aaaaaaacaa tgggatggga
     2581 agctagtaca gaaagattat atccaagaga cggggtttta aagggagaaa cacataaagc
     2641 tctaaaatta aaggacggag gacattattt agttgaattt aaatcaattt atatggctaa
     2701 aaaaccagtt caattaccag gatattatta tgtagatgct aagttagata ttacatcaca
     2761 taatgaagat tatacaattg ttgaacaata tgaaagaaca gaaggtagac atcatttatt
     2821 tttatagcat tcgtagaatt cacaattcga ttatatttat actggactat ttttacatct
     2881 gttcggttat tttcacattt atttttctat atatatctta taaacgtttt aaaacccatg
     2941 taatttttgt taagctgtaa tataaaagac gtcctaacaa acttctttta ttactgaatt
     3001 tcctttaatt ataataaata acaagtttta aaataaattc aggcaattaa ggcgctcctg
     3061 aggtactaaa attaatgtaa acatttaaaa ttaacttgga tggtcttaag tactgtactc
     3121 gtgattttgt tatactttat tattagaaaa gtcgtctatt aactttttgt tccttaattt
     3181 acttgattaa attgtcgctt aatttatcaa atcaggtttt gcgcgttatt ttagagaaaa
     3241 acttattaga aaaatgaata agcaaagttt aggctaacat gtttttttat tattttaaat
     3301 agttcaagtc aatgacgtat aaaccagctt ttgttccctt tagtgagggt taattgcgcg
     3361 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3421 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3481 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3541 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3601 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3661 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3721 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3781 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3841 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3901 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3961 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4021 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4081 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4141 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4201 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4261 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4321 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4381 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4441 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4501 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4561 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4621 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4681 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4741 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4801 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4861 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4921 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4981 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5041 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5101 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5161 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5221 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5281 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5341 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5401 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5461 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5521 gccac
//
