LOCUS       V015755                 4514 bp    DNA     circular SYN 12-MAY-2020
DEFINITION  Exported.
ACCESSION   V015755
VERSION     V015755
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
   PUBMED   32341562
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 4514)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways,
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
FEATURES             Location/Qualifiers
     source          1..4514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1112
                     /gene="hpaC"
                     /label="4-hydroxyphenylacetate 3-monooxygenase reductase
                     component"
                     /note="4-hydroxyphenylacetate 3-monooxygenase reductase
                     component from Escherichia coli. Accession#: Q57501"
     misc_feature    1122..1178
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    1302..1423
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1430..1885)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1912..2016
                     /label="AmpR promoter"
     promoter        2018..2375
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2410..3201
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3436..3483
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3812..4400
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatgcagct tgatgagcag cgtttgcgtt ttcgtgacgc tatggcatca cttagtgccg
      661 ctgtgaacat catcacgact gagggagacg ccggtcagtg cggaatcaca gcaaccgcag
      721 tatgtagtgt tacggacact cccccatctc tgatggtctg tatcaatgcc aatagtgcta
      781 tgaatccagt ctttcagggt aatggtaaac tttgtgtcaa cgtcctgaat cacgagcaag
      841 agttgatggc tcgtcatttt gcaggaatga ctggtatggc tatggaagag cgtttttcct
      901 tgtcttgttg gcaaaaagga ccactggccc agcctgtatt gaaaggttct ttggcttccc
      961 tggagggtga gatccgtgac gttcaagcca ttggaactca tctggtatat ttggtagaaa
     1021 tcaagaatat tatcctgtca gccgagggcc atggtctgat ctactttaaa cgtcgttttc
     1081 accccgtgat gctggagatg gaggcagcca tttcaggttg aagatctcga gctcaagctt
     1141 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
     1201 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1261 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     1321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc
     1441 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1501 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1561 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1621 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1681 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1741 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1801 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1861 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1981 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     2041 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2101 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     2161 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     2221 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2281 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2341 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     2401 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     2461 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     2521 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2581 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2641 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2701 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2761 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2821 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2881 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     2941 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     3001 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     3061 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     3121 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     3181 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     3241 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     3301 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     3361 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     3421 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     3481 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     3541 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     3601 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     3661 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3721 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4441 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4501 ttaccgccat gcat
//
