LOCUS       V016108                 5564 bp    DNA     circular SYN 12-MAY-2020
DEFINITION  Exported.
ACCESSION   V016108
VERSION     V016108
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
   PUBMED   32341562
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 5564)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways,
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
FEATURES             Location/Qualifiers
     source          1..5564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..2162
                     /gene="hpaB"
                     /label="4-hydroxyphenylacetate 3-monooxygenase oxygenase
                     component"
                     /note="4-hydroxyphenylacetate 3-monooxygenase oxygenase
                     component from Escherichia coli. Accession#: Q57160"
     misc_feature    2172..2228
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    2352..2473
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2480..2935)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2962..3066
                     /label="AmpR promoter"
     promoter        3068..3425
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3460..4251
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4486..4533
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4862..5450
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatgaaacc tgaagatttt cgtgcttcca cccagcggcc ctttactgga gaagaatatc
      661 tgaagtctct tcaagacggc cgtgagatct atatttacgg cgagcgtgta aaggatgtta
      721 caacgcaccc tgcttttcgt aatgctgctg cctcagttgc ccaactttat gacgccctgc
      781 ataaacccga aatgcaggac tcactttgtt ggaatacgga cacggggtcc ggtggttaca
      841 cacacaagtt ctttcgtgtc gccaaatctg ccgatgactt gcgtcaacaa cgtgatgcta
      901 tcgctgaatg gtcacgtctg agttacggct ggatgggccg tacgcccgac tataaggccg
      961 cctttggttg cgcattgggt gcaaatcccg gcttttatgg acagtttgaa caaaatgcac
     1021 gtaactggta tactcgtatc caggaaaccg gcttgtactt taatcatgct attgttaacc
     1081 cacctatcga tcgtcatttg ccaaccgata aggtcaagga tgtatatatt aaacttgaaa
     1141 aggagacgga cgctggtatc attgtgtctg gcgctaaagt ggtggctacg aacagtgcat
     1201 tgacgcatta caatatgatt ggttttggtt ccgcccaagt gatgggcgag aaccccgact
     1261 ttgccttgat gtttgttgcc cctatggacg ctgacggagt aaaacttatt tcacgtgcct
     1321 catatgaaat ggtagctggt gccactggtt ctccttatga ttatcctctt tcatcccgtt
     1381 ttgacgaaaa cgacgccatc ctggtcatgg acaatgttct tattccctgg gagaacgtgc
     1441 tgatctaccg tgacttcgat cgttgccgtc gttggactat ggaaggaggt ttcgctcgta
     1501 tgtatccact gcaggcctgt gttcgtttgg ctgtgaagtt ggactttatc acagcccttc
     1561 ttaaaaagtc acttgagtgt actggaacgt tggagttccg tggtgtacag gcagatctgg
     1621 gcgaagtagt agcctggcgt aatactttct gggcactgtc tgactctatg tgctccgagg
     1681 caacaccatg ggtgaacggc gcatacctgc ccgatcacgc cgctcttcaa acatatcgtg
     1741 tgcttgctcc catggcttat gctaaaatca aaaatattat cgagcgtaat gtaacttctg
     1801 gtctgattta cctgccatcc agtgctcgtg accttaacaa tcctcaaatc gatcagtatc
     1861 ttgcaaaata tgtgcgtggc tcaaacggaa tggaccatgt gcagcgtatc aaaatcctga
     1921 agttgatgtg ggatgccatc ggttctgaat ttggcggacg tcatgaactg tatgagatta
     1981 actactctgg atctcaagac gaaatcagac ttcagtgttt gcgtcaggcc caaaattctg
     2041 gaaatatgga taaaatgatg gctatggtag accgttgtct gagtgagtac gatcaagatg
     2101 gttggacagt tcctcatctt cataataatg acgatattaa tatgcttgat aagttgctta
     2161 aatcaggttg aagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     2221 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     2281 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2341 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2461 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     2521 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2581 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2641 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     2701 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2761 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     2821 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     2881 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     2941 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3001 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3061 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3481 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3541 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3601 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     3661 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3721 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3781 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3841 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3901 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     3961 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     4021 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4081 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4141 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4201 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4261 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4321 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4381 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     4441 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4501 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4561 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4621 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4681 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     4741 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4801 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4921 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4981 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5041 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5101 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5161 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5221 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5281 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5341 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5401 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5461 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5521 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
