LOCUS       40924_24792       12146 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pHonda1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Sayal R, Ryu SM, Arnosti DN.
  TITLE     Optimization of reporter gene architecture for quantitative 
            measurements of gene expression in the Drosophila embryo
  JOURNAL   Fly (Austin) 5 (1), 47-52 (2011)
  PUBMED    21150286
REFERENCE   2  (bases 1 to 12146)
  AUTHORS   Sayal R, Ryu SM, Arnosti DN.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Biochemistry and Molecular Biology, Michigan
            State University, 413 Biochemistry, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 12146)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin)"; date: "2011"; volume: "5"; issue: "1"; pages: "47-52"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2011) Biochemistry and Molecular Biology, Michigan State 
            University, 413 Biochemistry, East Lansing, MI 48824, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_structure  4849..4914
                     /label=enhancer MCS
                     /note="enhancer MCS"
     misc_structure  4915..5254
                     /label=Knirps ORF340 spacer
                     /note="Knirps ORF340 spacer"
     regulatory      5261..5360
                     /label=twist promoter
                     /note="twist promoter"
                     /regulatory_class="promoter"
     5'UTR           5367..5482
                     /note="even-skipped (eve) 5' UTR; fragment"
     misc_feature    5488..5493
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     CDS             5495..8539
                     /label=lacZ
                     /note="beta-galactosidase"
     gene            8660..8819
                     /gene="lacY"
                     /label=lacY
     misc_feature    8832..9631
                     /note="contains alpha-tubulin 3' UTR and 3' flanking
                     sequence"
     protein_bind    9670..9739
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(9958..9976)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9997..10013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10021..10037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10045..10075)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10090..10111)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10399..10987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11161..12018)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12019..12123)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccatgacc
     4861 ggtgatggcg cgcctgccct agggcattaa ttaatgcggc cggccgagga attcaaccgc
     4921 tttagtcccg ccagccagcc accagtggtg cagcccacct cctcggcccg ccagtcgccc
     4981 atcgatgtct gcctggagga ggatgttcac tccgtgcaca gccatcagtc gtccgcaagc
     5041 ctcctgcatc ccattgccat ccgagccacg ccaaccactc cgactagcag cagcccgctg
     5101 agttttgcgg ccaagatgca gagcttgtcg cccgtttcgg tttgctccat tggcggcgaa
     5161 accaccagcg ttgtaccagt gcatcctccc accgtttccg ctcaagaagg acccatggat
     5221 ctgagcatga agacctcgcg gagctccgtg cacaagatct gagcagccgc aaaatgtcaa
     5281 tttgagcaat ggccggaagg atcctgcgtc agttgcgttc cgtaagtgcg tgcgagcaga
     5341 tcgatccagc aaaacgcggg ctcgagttaa tatcctctga ataagccaac tttgaatcac
     5401 aagacgcata ccaaacatgc acggataccg aacctacaac atggagagcc accatgccca
     5461 tcacgacgcc agtcccgtgg acctagagga tcccgtcgtt ttacaacgtc gtgactggga
     5521 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5581 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5641 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     5701 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     5761 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     5821 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     5881 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     5941 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     6001 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     6061 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     6121 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     6181 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     6241 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     6301 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     6361 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     6421 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     6481 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     6541 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     6601 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     6661 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     6721 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     6781 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     6841 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     6901 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     6961 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     7021 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     7081 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     7141 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     7201 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     7261 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     7321 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     7381 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     7441 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     7501 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     7561 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     7621 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     7681 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     7741 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     7801 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     7861 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     7921 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     7981 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     8041 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     8101 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     8161 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     8221 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     8281 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     8341 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     8401 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     8461 ctcctggagc ccgtcagtat cggcggaatt acagctgagc gccggtcgct accattacca
     8521 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
     8581 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt
     8641 tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga
     8701 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc
     8761 gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggcaagc tctaggcccg
     8821 gtacctctag aagtactaag cgtcacgcca cttcaacgct cgatgggagc gtcattggtg
     8881 ggcggggtaa ccgtcgaaat cagtgtttac gcttccaatc gcaacaaaaa attcactgca
     8941 acactgaaaa gcatacgaaa acgatgaaga ttgtacgaga aaccataaag tattttatcc
     9001 acaaagacac gtatagcaga aaagccaagt taactcggcg ataagttgtg tacacaagaa
     9061 taaaatcggc cagattcagt gttgtcagaa ataagaaaac cccactgttt ttctttgcct
     9121 tttctttctc ccagcgatca ttcatttcgt ggtgaaagaa cggggtcatt gcacggagtt
     9181 tcgactgcgg gaaagcagag ctgccgttca cttcgtctat aattagcgct ttctattttc
     9241 cccgattcgg gccgctgctg cgcttttccg cctgctgttt gtggcaagtg tagcagcagg
     9301 ctgtgcacgc agtgtggcat gcacttggct ttccaccgtt ggtatcgatt ctctgggacg
     9361 atgagtcatt cctttcgggg ccacagcata atcgttgcca gctcaccgaa atggtgactt
     9421 catttcttaa ctgccgtcaa gcatgcgatt gtacatacat acatatttac atatgtacat
     9481 atttatgtga ctatggtagg tcgatataat agcaatcaac gcaagcaaat gtgtcagtcc
     9541 tgcttacagg aacgattcta tttagtaatt ttcgttgtat aaagtaatta tgtatgtatg
     9601 taagccccat aaatctgaaa caattaggca atctagagat ccactagtgt cgacgatgta
     9661 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     9721 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     9781 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     9841 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     9901 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     9961 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10021 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10081 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10141 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10201 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11041 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11101 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11161 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11221 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11281 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11341 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11401 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11461 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11521 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11581 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11641 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11701 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11761 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11821 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11881 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11941 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12001 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12061 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12121 gcgcacattt ccccgaaaag tgccac
//
