LOCUS       pHom-1.        5371 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding an FKBP homodimerizer domain, for creating
            soluble fusion proteins that can be dimerized with a drug. Also 
            known as pC4-FV1E.
ACCESSION   .
VERSION     .
KEYWORDS    pHom-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone into the XbaI site to fuse DmrB to the C-terminus of the 
            partner protein, or into the SpeI site to fuse DmrB to the 
            N-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             676..678
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             688..1008
                     /label=DmrB
                     /note="F36V mutant of FK506-binding protein FKBP12"
     CDS             1015..1041
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1073..1645
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    1842..1897
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(2250..2385)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2662..3250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3421..4293)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4294..4398)
                     /label=AmpR promoter
     rep_origin      complement(4730..5185)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5330..5346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5353..5371
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 ccagaagccg ccaccatggc ttctagagga gtgcaggtgg agactatctc cccaggagac
      721 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      781 gatggaaaga aagttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      841 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      901 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      961 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagttatccg
     1021 tacgacgtac cagactacgc ataagaaaag tgaggatcct gagaacttca gggtgagttt
     1081 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     1141 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     1201 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     1261 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     1321 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     1381 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     1441 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     1501 tagaaacaac tacatcctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     1561 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     1621 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     1681 tcattttggc aaaggattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     1741 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     1801 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     1861 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     1921 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
     1981 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
     2041 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     2101 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     2161 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
     2221 ccctcgagga gctttttgca aaagccctag gcctccaaaa aagcctcttc actacttctg
     2281 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     2341 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     2401 cgggactatg gttgctgact aattgctgca ttaatgaatc ggccaacgcg cggggagagg
     2461 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2521 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2581 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2641 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2701 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2761 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2821 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2881 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2941 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3001 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3061 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3121 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3181 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3241 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3301 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3361 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3421 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3481 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3541 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3601 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3661 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3721 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3781 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3841 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3901 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3961 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4021 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4081 catcattgcc acctccacct gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4441 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     4501 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     4561 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4621 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     4681 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaaac
     4741 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4801 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4861 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4921 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4981 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     5041 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     5101 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5161 cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg
     5221 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5281 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5341 gccagtgaat tgtaatacga ctcactatag g
//
