LOCUS       pHet-Nuc1.        5410 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding an FKBP heterodimerizer domain, for 
            creating soluble fusion proteins that can be dimerized with a drug. 
            Also known as pC4EN-F1.
ACCESSION   .
VERSION     .
KEYWORDS    pHet-Nuc1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone into the XbaI site to fuse DmrA to the C-terminus of the 
            partner protein, or into the SpeI site to fuse DmrA to the 
            N-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             673..675
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             685..711
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             733..753
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             760..1080
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     intron          1112..1684
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    1881..1936
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(2289..2424)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2701..3289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3460..4332)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4333..4437)
                     /label=AmpR promoter
     rep_origin      complement(4769..5224)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5369..5385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5392..5410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 ccagaagcgc gtatggcttc tagctatcct tatgacgtgc ctgactatgc cagcctggga
      721 ggaccttcta gtcctaagaa gaagagaaag gtgtctagag gagtgcaggt ggaaaccatc
      781 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
      841 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag
      901 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
      961 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     1021 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     1081 actagttatt gaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     1141 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     1201 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     1261 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     1321 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     1381 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     1441 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     1501 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     1561 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     1621 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aaggattcac
     1741 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1801 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1861 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1921 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1981 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     2041 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     2101 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     2161 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     2221 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgaggag ctttttgcaa
     2281 aagccctagg cctccaaaaa agcctcttca ctacttctgg aatagctcag aggccgaggc
     2341 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     2401 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     2461 attgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3121 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattgcca cctccacctg
     4141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4681 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4741 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
     4801 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4861 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4921 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4981 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5041 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5101 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5161 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5221 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5281 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5341 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     5401 tcactatagg
//
