LOCUS       pHet-Mem1.        5726 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding FKBP heterodimerizer domains, for creating
            membrane-associated fusion proteins that can be dimerized with a 
            drug. Also known as pC4M-F2E.
ACCESSION   .
VERSION     .
KEYWORDS    pHet-Mem1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone into the XbaI site to fuse the DmrA domains to the C-terminus 
            of the partner protein, or into the SpeI site to fuse the DmrA 
            domains to the N-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             668..709
                     /label=myr
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             716..1036
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     CDS             1043..1363
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=human FK506-binding protein FKBP12
                     /note="FKBP (DmrA)"
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1370..1396
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1428..2000
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    2197..2252
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(2605..2740)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3017..3605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3776..4648)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4649..4753)
                     /label=AmpR promoter
     rep_origin      complement(5085..5540)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5685..5701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5708..5726
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 cgcgcgtatg gggagtagca agagcaagcc taaggacccc agccagcgct ctagaggagt
      721 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
      781 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag
      841 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
      901 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
      961 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
     1021 gcttctaaaa ctggaaacta gaggcgtgca ggtcgagacc atcagccccg gcgacggccg
     1081 cacctttccc aagagaggcc agacttgcgt ggtccactac accggcatgc tggaggacgg
     1141 caagaagttc gacagcagcc gcgaccgcaa caagcccttc aagttcatgc tgggcaaaca
     1201 ggaagtgatc cgcggctggg aggaaggcgt ggctcagatg agcgtggggc agcgggccaa
     1261 gctgaccatc agccccgact atgcctacgg cgccaccggc caccccggca tcatcccccc
     1321 ccacgccacc ctggtgttcg acgtggagct gctgaagctg gagactagtt atccgtacga
     1381 cgtaccagac tacgcataag aaaagtgagg atcctgagaa cttcagggtg agtttgggga
     1441 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
     1501 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     1561 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     1621 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     1681 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     1741 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     1801 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     1861 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     1921 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     1981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt
     2041 ttggcaaagg attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2101 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2161 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     2221 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     2281 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc
     2341 tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2401 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2461 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2521 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg aagatccctc
     2581 gaggagcttt ttgcaaaagc cctaggcctc caaaaaagcc tcttcactac ttctggaata
     2641 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     2701 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     2761 ctatggttgc tgactaattg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3421 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4441 ttgccacctc cacctgaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4801 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4861 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4921 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4981 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5041 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa
     5101 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5161 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5221 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5281 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5341 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5401 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5461 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5521 tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
     5581 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5641 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5701 tgaattgtaa tacgactcac tatagg
//
