LOCUS       pHet-Act1-2.        8202 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a transcription factor activation domain 
            and a DNA-binding domain that can be assembled by drug-induced 
            heterodimerization.
ACCESSION   .
VERSION     .
KEYWORDS    pHet-Act1-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1102..1104
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1114..1392
                     /label=FRB
                     /note="FKBP-rapamycin binding domain of human FRAP"
     CDS             1600..1971
                     /label=RelA (p65) AD
                     /note="transcriptional activation domain of human RelA,
                     also known as p65 (O'Shea and Perkins, 2008)"
     misc_feature    2040..2626
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2647..2649
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2656..2682
                     /label=c-myc NLS
                     /note="nuclear localization signal of human c-Myc
                     proto-oncogene (Dang and Lee, 1988)"
     CDS             2689..3051
                     /label=ZFHD1 (DNA binding domain)
                     /note="composite human DNA-binding domain composed of
                     domains from the transcription factors Zif268 and Oct-1 
                     (Pomerantz et al., 1995)"
     CDS             3058..3378
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     CDS             3388..3705
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /note="FKBP (DmrA)"
                     /translation="VQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPF
                     KFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLK
                     LE"
     CDS             3712..4032
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /note="FKBP (DmrA)"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    complement(4101..4222)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4408..4863
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4980..5337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5388..5984
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    6051..6099
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        6405..6509
                     /label=AmpR promoter
     CDS             6510..7367
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7541..8129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgagaatt catggcttct agaatcctct ggcatgagat gtggcatgaa
     1141 ggcctggaag aggcatctcg tttgtacttt ggggaaagga acgtgaaagg catgtttgag
     1201 gtgctggagc ccttgcatgc tatgatggaa cggggccccc agactctgaa ggaaacatcc
     1261 tttaatcagg cctatggtcg agatttaatg gaggcccaag agtggtgcag gaagtacatg
     1321 aaatcaggga atgtcaagga cctcctccaa gcctgggacc tctattatca tgtgttccga
     1381 cgaatctcaa agactagaga tgagtttccc accatggtgt ttccttctgg gcagatcagc
     1441 caggcctcgg ccttggcccc ggcccctccc caagtcctgc cccaggctcc agcccctgcc
     1501 cctgctccag ccatggtatc agctctggcc caggccccag cccctgtccc agtcctagcc
     1561 ccaggccctc ctcaggctgt ggccccacct gcccccaagc ccacccaggc tggggaagga
     1621 acgctgtcag aggccctgct gcagctgcag tttgatgatg aagacctggg ggccttgctt
     1681 ggcaacagca cagacccagc tgtgttcaca gacctggcat ccgtcgacaa ctccgagttt
     1741 cagcagctgc tgaaccaggg catacctgtg gccccccaca caactgagcc catgctgatg
     1801 gagtaccctg aggctataac tcgcctagtg acaggggccc agaggccccc cgacccagct
     1861 cctgctccac tgggggcccc ggggctcccc aatggcctcc tttcaggaga tgaagacttc
     1921 tcctccattg cggacatgga cttctcagcc ctgctgagtc agatcagctc cactagttat
     1981 taagaattca cgcgtcgagc atgcatctag ggcggccaat tccgcccctc tccccccccc
     2041 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     2101 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     2161 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     2221 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     2281 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     2341 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     2401 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     2461 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     2521 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     2581 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccggg atcctctaga
     2641 gtcgacatgg actatcctgc tgccaagagg gtcaagttgg actctagaga acgcccatat
     2701 gcttgccctg tcgagtcctg cgatcgccgc ttttctcgct cggatgagct tacccgccat
     2761 atccgcatcc acacaggcca gaagcccttc cagtgtcgaa tctgcatgcg taacttcagt
     2821 cgtagtgacc accttaccac ccacatccgc acccacacag gcggcggccg caggaggaag
     2881 aaacgcacca gcatagagac caacatccgt gtggccttag agaagagttt cttggagaat
     2941 caaaagccta cctcggaaga gatcactatg attgctgatc agctcaatat ggaaaaagag
     3001 gtgattcgtg tttggttctg taaccgccgc cagaaagaaa aaagaatcaa cactagagga
     3061 gtgcaggtgg aaaccatctc cccgggagac gggcgcacct tccccaagcg cggccagacc
     3121 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac
     3181 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa
     3241 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc
     3301 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg
     3361 gagcttctaa aactggaagt cgagggcgtg caggtggaaa ccatctcccc aggagacggg
     3421 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
     3481 ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     3541 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     3601 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     3661 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag aggagtgcag
     3721 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     3781 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
     3841 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     3901 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     3961 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     4021 ctaaaactgg aaactagtta ttaagtcgac ccgggcggcc gcttcccttt agtgagggtt
     4081 aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4141 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4201 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4261 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg
     4321 ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4381 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     4441 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4501 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4561 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4621 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4681 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4741 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4801 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4861 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     4921 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     4981 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5041 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5101 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5161 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5221 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5281 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5341 cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg accgagtaca
     5401 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     5461 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     5521 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5581 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5641 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5701 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5761 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5821 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5881 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5941 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg ggactctggg
     6001 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat
     6061 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc
     6121 gcgtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6181 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6241 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6301 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6481 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6541 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6601 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6661 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6721 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6781 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6841 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6901 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6961 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7021 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7081 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7141 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7201 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7261 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7321 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7381 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7441 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7501 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7561 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7621 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7681 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7741 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7801 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7861 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7921 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7981 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8041 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8101 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8161 tggccttttg ctggcctttt gctcacatgg ctcgacagat ct
//
