LOCUS       62056_12310        7532 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2011..3660
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          3903..3968
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4098..4118
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4543..4677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4990..5578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5752..6609)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6610..6714)
                     /label=AmpR promoter
     rep_origin      6741..7196
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccatcaagc ttaacgtatg actaattgtt ctggattctc tacatccttt tacttctgtc
       61 tactgcctct tttaaaggca ctcccagggg aatcttgtgg ggaatctata gatggcagcc
      121 ataccacaca gatacctggc aacgagcttt ctaaaggcac tgttaatgaa ctacacccga
      181 tgaaggcttc atgacctaaa ggcacccctc cggattcaac cccccgcaac acgatctaag
      241 cccccaaagg gaaaaacaga atccccaagc attgtccaga aagcccggtt ctgtgcgccc
      301 ctgcagtagg ttggagaata aatactccgt gggagcggcc actctatctc tcatcgtggt
      361 aatcccaggg tttaacagtg tctggcgttc catcacaaag gggggtgggg ggggcttgcc
      421 caaggtcatg cagtcaagga agcaccacct cgtccgggac cagagctgga gaaatctttc
      481 acatgatctc agtgggaggt tgggggaggg gacgaaggga cgagccggtg cgccttgtgt
      541 gccaggcctc ccggacgcgg gaaagactgg gggcggggat caaacaacgc gctgggatcc
      601 cgagctgccc cgagttcacg gcggcgggaa aactgagggg tctggggcac ccgggtggag
      661 gccagggcag gcaggaggga ggcaggcagc cgcccgggca gtgcgcaccg cccgcgccgc
      721 gcggcaaatc ccccagggag gccgccgcca gagccctggc gctcccggcc cggcgcccgc
      781 cacgtggcac ccggcggcgg cggcgactcc cgggggcgcg cagcacacac gactcagccg
      841 acacgcacgc agcacacaca catctcttct gggccgtccg gccggcagcc gcttggacgc
      901 agcaataaaa catcctggca cgtgctccga ctccaggcgc gccctcgccg gccggctgat
      961 gtcaaactgc agctcggctg gtggagctct taaagggctc gcgcgcgggg cgccgaggcc
     1021 gccttggccg cccgcggggt gaggagggga gagccccgac tcccttgccc gccgtctatc
     1081 cgtattgcca actgggagcc tgcggggaga aaggtagaga gagtggcgga ggaacgagcg
     1141 cgcccctcct gtcgcattgc tatcaaagcc ccccttgttc attttttttt tcgtttgaaa
     1201 ttccatttcc tttctgccca gtaggaaatt attctgattg ttttctggag aagcgtgagg
     1261 cccaaggttg caattccaag agaggaatga atgggctaga gggagacaag catccaaaag
     1321 acaccttgct ccgaaaaccc tggctgtgaa ggcatttggg gggacggtaa gggcatgttt
     1381 agcgtgtggg gtttagtatc tccccaacct catgagcagt tttgactccc ctaaacatac
     1441 agagttcgag cgggaattcc ggaagacatt agagtaacac atcctcttta ccttgttccc
     1501 tccttttttc aatcactaaa ttttgtcttg gcctatatct gttcaaaata tttttcaaat
     1561 gaacttatta tacaaagtag ttatattgca tgcagcaaga acaataaaaa ccaaaggcct
     1621 ggccacaaaa gaaatagact agactaaaac taagcaaagc ccagaggaaa gagttagcaa
     1681 agggttaaaa tccttttgat tgacgttgta gcctccggtg ccccgggctc aggcgcgcgc
     1741 cattggccgc cagaccttgt gcctagcggc caatgggggg gcgcagtcca cgagcggtgc
     1801 cgcgtgtctc ttcctcccat tggctgaaag ttactgtggg aaagaaagtt tgggaagttt
     1861 cacacgagcc gttcgcgtgc agtcccagat atatatagag gccgccaggg cctgcggatc
     1921 acacaggatc tggagctggt gctgataaca gcggaatccc ctgtctggtc gagatctaag
     1981 taagcttggc attccggtac tgttggtaaa atggaagacg ccaaaaacat aaagaaaggc
     2041 ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca taaggctatg
     2101 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac
     2161 atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     2221 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     2281 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     2341 gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa
     2401 aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt
     2461 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     2521 catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa
     2581 acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt
     2641 ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa
     2701 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     2761 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     2821 gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc
     2881 ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     2941 gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc
     3001 ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg
     3061 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     3121 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta
     3181 tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     3241 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     3301 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
     3361 cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca
     3421 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     3481 aagacgatga cggaaaaaga gatcgtggat tacgtggcca gtcaagtaac aaccgcgaaa
     3541 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     3601 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
     3661 taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatac tgcgatgagt
     3721 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta
     3781 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg
     3841 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     3901 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     3961 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4021 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4081 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4141 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4201 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4261 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     4321 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     4381 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     4441 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4501 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4561 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4621 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt
     4681 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     4741 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     4801 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4861 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4921 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4981 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5041 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5101 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5161 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     5221 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5281 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5341 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5401 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5461 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5521 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5581 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5641 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5701 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5761 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5821 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5881 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5941 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6001 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6061 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6121 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6181 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6241 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6301 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6361 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6421 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6481 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6541 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6601 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6661 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6721 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6901 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7021 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7081 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7141 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca
     7201 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     7261 acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag gttttacttg
     7321 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     7381 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7501 tatcttatgg tactgtaact gagctaacat aa
//
