LOCUS       40924_24443        5510 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pHel3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Haas R, Heuermann D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2017) Genetics and Genomics, Icahn School of 
            Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029, USA
REFERENCE   2  (bases 1 to 5510)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5510)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2017) Genetics and Genomics, Icahn School of Medicine at 
            Mount Sinai, 1425 Madison Ave, New York, NY 10029, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: HGAP v. 3
            Coverage              :: 100x
            Sequencing Technology :: PacBio
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..76
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            1095..1204
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             2082..3701
                     /codon_start=1
                     /product="RepA"
                     /label=RepA
                     /protein_id="ATY70547.1"
                     /translation="MPMNTNFEQLRKQELELRKLLEELETLPQTPQIKLQKQKIQTYID
                     KITPSILSGFDQKFKEIIENLSNEFEKEKSTPLKEPQTTPTPCKDLVVSTPKDNTYTTY
                     HNNANKVNLGKLSEREANLLFAIFQKLKDQGNTLIRFEPQDLKRMIMVKSNLTNRQLLQ
                     ILKNLLDNIGGANFWIIREHVENGEIYEDHTSYMLFKQFDIRIHKPTQTIEYLEVQLND
                     SYHYLLNNLGMGGQYTSFKLLEFQRVRGKYAKTLYRLLKQYKSTGILSVEWDQFRELLD
                     IPKDYKMENIDQKVLTPSLKELRKIYPFEHLSYKKERKSHDKRKVTHIDFYFEQLPQGE
                     TKKQKQADKQRAQRDIKLIAWDIHNQIAKRNAKATIEARFLELKTLIGYQFRHNNGTIL
                     QIDNATFEKNQMFLHVSTSKNSQKFLVSNKTFALELLFVNGYSLKKDMLEIDPPSIHPI
                     TNEPIKEFAEYIGKTIHITNFNVDQCPEGINNYLKITRIVKLNDNRICVSVQDVDKPEK
                     LLKPFIAKDEKHLKNWFKKHYR"
     CDS             complement(4230..5021)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tcaaatggtt cgctgggttt gaattcgagc tcggtacccg gggatcctct agagtcgacc
       61 tgcaggcatg caagctagct ttcgcgagct cgagatctag atatcgatga attcaaaaaa
      121 aaaggctcca aaaggagcct ttaatgatct aattcgtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccaccggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 ggacagtatt tggtatctgc gctctgctga aaccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatctctgca gttcagtaat
      901 ttcctgcatt tgcctgtttc cagtcggtag atattccaca aaacagcang gaagcagcgc
      961 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     1021 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     1081 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     1141 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     1201 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagctgcag
     1261 aagcttgtcc gttagcaaat ataatgtgtt tagcacctaa aagcgcatct atcttgtgtt
     1321 agccatctaa cctaaacaaa accgcctagc gagcgttagc gagcatggac aaaagcgcat
     1381 cgcagtttga aatcgtaggc gttagcagga tggggttttg cgttagcaaa atcaaacaag
     1441 atagcggaaa accggggggg tcaccaaaaa aaaccctaaa actaaaatcc ccaaaatatg
     1501 tagcgcgtca tgcgcgttgt ttttattaca tgttttaata actatgttgt ttttacatgt
     1561 ttttacaacg cgcgcgcgtg tgagggattg ggggttgcaa ccccctaaat aacgaagctg
     1621 tagggtttct catttttggg ttaaaatgaa taaaacagaa cttcttacga gtgataacag
     1681 aacttcttac gagtgataac agaacttctt acgagtgata acagaacttc ttacgagtga
     1741 taacagaact tctttattgt gaagttatga ttattaacaa tttttagcta aaataacaac
     1801 ctaaggcggt gtaacatggt tgctaatcct agtgttaaca aatttggagc aattagcttt
     1861 aaaagctagt gggttgggag tttgtagcgg gtatgcactc cgttaggagg cacaccatga
     1921 aagcattttt gatagtagtg attttagtgg taatcttgac acagccacta tattaaaacc
     1981 ttagcgtttt aataaccctt ataagtccgc caagacttct taagggtttc actcctgtta
     2041 ttatatcgtc ttttgaaaaa taagcattaa aaggcgctta aatgcccatg aatacgaatt
     2101 ttgaacagct tagaaaacaa gaattggaat tacgaaaatt attagaagaa ttagaaacgc
     2161 tcccacaaac cccacaaatt aaactgcaaa aacaaaaaat acaaacttac atagacaaga
     2221 taacaccaag tattttgagc ggttttgatc aaaaattcaa agaaattata gaaaatctat
     2281 caaatgaatt tgaaaaagaa aaatccacac cactcaaaga gccacaaacc acccccacac
     2341 catgcaaaga tttagtggtt agcaccccta aagataacac ctataccacc taccacaata
     2401 acgctaataa ggtcaatcta gggaaattga gcgaaaggga agccaatctt ttattcgcta
     2461 tttttcaaaa actcaaagat caagggaata ccctcattcg ttttgaaccg caagatttga
     2521 aacgcatgat catggtcaaa tccaacctaa ccaacagaca attattgcaa atcctcaaaa
     2581 acttacttga caacataggc ggtgctaatt tttggatcat tagagagcat gttgaaaatg
     2641 gcgaaatcta tgaagatcac actagctaca tgcttttcaa acaatttgac attcgtatcc
     2701 ataagccaac ccaaactata gaatacttag aagtccaact caacgatagc taccactacc
     2761 tgctcaacaa tctaggcatg ggcggtcaat acacttcctt taaactttta gaattccaaa
     2821 gggtgagggg caaatacgcc aagacgctct atcgcttact caagcaatac aaaagcacag
     2881 ggattttgag cgtggaatgg gatcaattca gggagctttt agacattcca aaagattaca
     2941 aaatggaaaa catcgatcaa aaagtcttaa ctccaagcct caaggaactc agaaaaatct
     3001 atccctttga acacttgagc tacaaaaaag aacgcaaaag ccacgacaaa cgcaaggtaa
     3061 cgcatattga tttttatttt gagcaattgc cacagggcga aaccaagaaa caaaaacaag
     3121 ccgacaagca acgcgctcaa agggacatca agctcatagc gtgggatatt cacaaccaaa
     3181 tcgctaaaag aaacgctaaa gccactatag aagctaggtt tcttgagcta aaaaccttga
     3241 ttggttatca gttcaggcac aacaatggga ctattttaca gattgacaac gccacttttg
     3301 aaaagaatca aatgtttttg catgtttcaa ccagcaaaaa ttctcaaaaa ttccttgtgt
     3361 ccaacaaaac attcgcttta gaacttctgt ttgtcaatgg atacagccta aaaaaagaca
     3421 tgttggaaat tgatccccca agtatccacc ccatcaccaa cgaacctatc aaggaatttg
     3481 cagaatacat cggaaaaacg atccacatca ccaatttcaa tgtggatcaa tgccctgagg
     3541 ggatcaataa ctacctgaaa atcactagga ttgtgaaact gaatgacaat cggatctgtg
     3601 tttcagtcca agatgtggat aagcctgaga aacttctaaa acctttcatt gctaaagatg
     3661 aaaaacattt gaaaaattgg tttaagaaac actatcggtg aaaaataagt ggtgggatta
     3721 acaatcgtcc cccacacctt gcaatgtagt tagttttgca ttattttttc tataatctct
     3781 atcaattctg agagaacata gattaattca atgaaatttt tcattatcta tttcctttct
     3841 gtaaattttg ttaaatattt attaaaaaaa aatttaaaga tcactcttta agtggaacat
     3901 acaaaggctg tagagagtgc cacattaagc ctgatgtttt gcttgtgtat agagtgaaag
     3961 gcaatatttt aactttagtt aggcttggca gtcatagtga attgttttag acaaatttct
     4021 ttctaaaatc gcccagaatg aatttttaat tctaatttaa tataattcat cggttttcac
     4081 tcaaaacgat gaaatagggc tatttttagc tcattattgg ggtattttgt ttttagttga
     4141 aagctaactt caaaattcaa aaaaacttca tttccctttc tagaggatcg atccccgggg
     4201 atcttttaga catctaaatc taggtactaa aacaattcat ccagtaaaat ataatatttt
     4261 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     4321 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     4381 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     4441 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     4501 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     4561 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     4621 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     4681 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     4741 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     4801 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     4861 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     4921 aacttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     4981 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     5041 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     5101 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     5161 agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta tgatagaatt
     5221 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
     5281 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
     5341 tacatacggc gatatcttct atataagcgt accggttcca attttttcgc agtttaactt
     5401 ttccgacgca tttatcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg
     5461 taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
//
