LOCUS       40924_24373        3322 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing HcRed1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..972
                     /codon_start=1
                     /label=HcRed1
                     /note="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     promoter        1417..1521
                     /label=AmpR promoter
     CDS             1522..2379
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2553..3141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcggc
      301 ctgctgaagg agagtatgcg catcaagatg tacatggagg gcaccgtgaa cggccactac
      361 ttcaagtgcg agggcgaggg cgacggcaac cccttcgccg gcacccagag catgagaatc
      421 cacgtgaccg agggcgcccc cctgcccttc gccttcgaca tcctggcccc ctgctgcgag
      481 tacggcagca ggaccttcgt gcaccacacc gccgagatcc ccgacttctt caagcagagc
      541 ttccccgagg gcttcacctg ggagagaacc accacctacg aggacggcgg catcctgacc
      601 gcccaccagg acaccagcct ggagggcaac tgcctgatct acaaggtgaa ggtgcacggc
      661 accaacttcc ccgccgacgg ccccgtgatg aagaacaaga gcggcggctg ggagcccagc
      721 accgaggtgg tgtaccccga gaacggcgtg ctgtgcggcc ggaacgtgat ggccctgaag
      781 gtgggcgacc ggcacctgat ctgccaccac tacaccagct accggagcaa gaaggccgtg
      841 cgcgccctga ccatgcccgg cttccacttc accgacatcc ggctccagat gctgcggaag
      901 aagaaggacg agtacttcga gctgtacgag gccagcgtgg cccggtacag cgacctgccc
      961 gagaaggcca actgaagcgg ccgcgactct agaattccaa ctgagcgccg gtcgctacca
     1021 ttaccaactt gtctggtgtc aaaaataata ggcctactag tcggccgtac gggccctttc
     1081 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     1141 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     1201 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     1261 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1321 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1381 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2041 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3181 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3241 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3301 tcagtgagcg aggaagcgga ag
//
