LOCUS       40924_24358        4118 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless HcRed1 reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Can be used to monitor transcription from a promoter inserted into 
            the MCS.
FEATURES             Location/Qualifiers
     source          1..4118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..780
                     /codon_start=1
                     /label=HcRed1
                     /note="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     polyA_signal    906..1027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1034..1489)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1516..1620
                     /label=AmpR promoter
     promoter        1622..1979
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2014..2805
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3040..3087
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3416..4004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcggcct gctgaaggag
      121 agtatgcgca tcaagatgta catggagggc accgtgaacg gccactactt caagtgcgag
      181 ggcgagggcg acggcaaccc cttcgccggc acccagagca tgagaatcca cgtgaccgag
      241 ggcgcccccc tgcccttcgc cttcgacatc ctggccccct gctgcgagta cggcagcagg
      301 accttcgtgc accacaccgc cgagatcccc gacttcttca agcagagctt ccccgagggc
      361 ttcacctggg agagaaccac cacctacgag gacggcggca tcctgaccgc ccaccaggac
      421 accagcctgg agggcaactg cctgatctac aaggtgaagg tgcacggcac caacttcccc
      481 gccgacggcc ccgtgatgaa gaacaagagc ggcggctggg agcccagcac cgaggtggtg
      541 taccccgaga acggcgtgct gtgcggccgg aacgtgatgg ccctgaaggt gggcgaccgg
      601 cacctgatct gccaccacta caccagctac cggagcaaga aggccgtgcg cgccctgacc
      661 atgcccggct tccacttcac cgacatccgg ctccagatgc tgcggaagaa gaaggacgag
      721 tacttcgagc tgtacgaggc cagcgtggcc cggtacagcg acctgcccga gaaggccaac
      781 tgaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      841 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      901 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1021 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     1081 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     1141 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     1201 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     1261 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     1321 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     1381 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     1441 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     1501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1621 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     1681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     1741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     1981 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     2041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     2101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     2161 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     2221 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     2281 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     2341 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     2401 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     2461 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     2521 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     2581 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     2641 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     2701 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     2761 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     2821 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     2881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     2941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     3001 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     3061 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     3121 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     3181 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     3241 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     3301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4081 cccctgattc tgtggataac cgtattaccg ccatgcat
//
