LOCUS       62056_12160        5841 bp DNA     circular SYN 14-DEC-2020
DEFINITION  Cloning vector pHal7-RFP, complete sequence.
ACCESSION   MW076824
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Trisrivirat D, Hughes JMX., Hoeven R, Faulkner M, Toogood H, Chaiyen
            P, Scrutton NS.
  TITLE     Promoter engineering for microbial bio-alkane gas production
  JOURNAL   Synth Biol (Oxf) 5 (1), ysaa022 (2020)
  PUBMED    33263086
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Trisrivirat D, Hughes JMX., Hoeven R, Faulkner M, Toogood HS, 
            Chaiyen P, Scrutton NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2020) Chemistry, University of Manchester, 131 
            Princess Street, Manchester, Lancashire M1 7DN, United Kingdom
REFERENCE   3  (bases 1 to 5841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)"; date: "2020"; volume: "5"; issue: "1"; pages: "ysaa022"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2020) Chemistry, University of Manchester, 131 Princess 
            Street, Manchester, Lancashire M1 7DN, United Kingdom"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(494..1324)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      1338..1689
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(1762..1848)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      1889..1959
                     /regulatory_class="promoter"
     CDS             1960..2634
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      2728..2822
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2931..3743
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4197..4299
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4519..5307
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     oriT            5488..5596
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5690..5841
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
       61 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggcatccg gtaagcggca
      301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      421 ggcggagcct atggaaaaac gccagcaacg cggccgtgaa aggcaggccg gtccgtggtg
      481 gccacggcct ctaggccaga tccagcggca tctgggttag tcgagcgcgg gccgcttccc
      541 atgtctcacc agggcgagcc tgtttcgcga tctcagcatc tgaaatcttc ccggccttgc
      601 gcttcgctgg ggccttaccc accgccttgg cgggcttctt cggtccaaaa ctgaacaaca
      661 gatgtgtgac cttgcgcccg gtctttcgct gcgcccactc cacctgtagc gggctgtgct
      721 cgttgatctg cgtcacggct ggatcaagca ctcgcaactt gaagtccttg atcgagggat
      781 accggccttc cagttgaaac cactttcgca gctggtcaat ttctatttcg cgctggccga
      841 tgctgtccca ttgcatgagc agctcgtaaa gcctgatcgc gtgggtgctg tccatcttgg
      901 ccacgtcagc caaggcgtat ttggtgaact gtttggtgag ttccgtcagg tacggcagca
      961 tgtctttggt gaacctgagt tctacacggc cctcaccctc ccggtagatg attgtttgca
     1021 cccagccggt aatcatcaca ctcggtcttt tccccttgcc attgggctct tgggttaacc
     1081 ggacttcccg ccgtttcagg cgcagggccg cttctttgag ctggttgtag gaagattcga
     1141 tagggacacc cgccatcgtc gctatgtcct ccgccgtcac tgaatacatc acttcatcgg
     1201 tgacaggctc gctcctcttc acctggctaa tacaggccag aacgatccgc tgttcctgaa
     1261 cactgaggcg atacgcggcc tcgaccaggg cattgctttt gtaaaccatt gggggtgagg
     1321 ccacgttcga cattccttgt gtataagggg acactgtatc tgcgtcccac aatacaacaa
     1381 atccgtccct ttacaacaac aaatccgtcc cttcttaaca acaaatccgt cccttaatgg
     1441 caacaaatcc gtcccttttt aaactctaga ggccacggat tacgtggcct gtagacgtcc
     1501 taaaaggttt aaaagggaaa aggaagaaaa gggtggaaac gcaaaaaacg caccactacg
     1561 tggccccgtt ggggccgcat ttgtgcccct gaaggggcgg gggaggcgtc tgggcaatcc
     1621 ccgttttacc agtcccctat cgccgcctga gagggcgcag gaagcgagta atcagggtat
     1681 cgaggcggat tcacccttgg cgtccaacca gcggcaccag cggcgcctga gaggggcgcg
     1741 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     1801 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt
     1861 aaagcggata acaatttcac acaggaggtt tttctattgc gtacaaccga taaaggtata
     1921 gagtttgaga ctttactaga gaaagaggag aaatactaga tggcgagtag cgaagacgtt
     1981 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     2041 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     2101 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     2161 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     2221 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     2281 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     2341 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     2401 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     2461 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     2521 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     2581 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaatta
     2641 acctaggctg ctgccaccgc tgagcaataa agcccatgca agcttgcggc cgcgtcgtga
     2701 ctgggaaaac cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag
     2761 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     2821 atccagacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2881 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     2941 ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat
     3001 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     3061 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     3121 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     3181 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     3241 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     3301 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     3361 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     3421 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     3481 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     3541 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     3601 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     3661 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     3721 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     3781 gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt ttgctgagtt
     3841 gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt
     3901 caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt
     3961 ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt cttcacgagg
     4021 cagacctcag cgctattctg accttgccat cacgactgtg ctggtcatta aacgcgtatt
     4081 caggctgacc ctgcgcgctg cgcagggctt tattgattcc atttttacac tgatgaatgt
     4141 tccgttgcgc tgcccggatt acagccggat cctctagagt cgacctgcag gcatgctgat
     4201 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     4261 tttgagttat cgagattttc aggagctaag gaagctaaaa tgcgctcacg caactggtcc
     4321 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     4381 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     4441 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     4501 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     4561 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     4621 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     4681 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     4741 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     4801 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     4861 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     4921 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     4981 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     5041 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     5101 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     5161 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     5221 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     5281 ggcgagatca ccaaggtagt cggcaaataa actagtaaat aataaaaaag ccggattaat
     5341 aatctggctt tttatattct ctgcataacc ctgcttcggg gtcattatag cgattttttc
     5401 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     5461 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     5521 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     5581 tctgcgaggc tggccgtagg ccggccgata atctcatgac caaaatccct taacgtgagt
     5641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5821 agataccaaa tactgttctt c
//
