LOCUS       40924_25271       13972 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pHZPRi-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Zhang H, Timko MP.
  TITLE     The tobacco transcription factors NtMYC2a and NtMYC2b regulate 
            multiple steps in nicotine biosynthesis and form nuclear complexes 
            with NtJAZ1 repressor
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13972)
  AUTHORS   Zhang H, Timko MP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2010) Biology, University of Virginia, Department 
            of Biology, University of Virginia, Charlottesville, VA 22904, USA
REFERENCE   3  (bases 1 to 13972)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13972)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUN-2010) Biology, University of Virginia, Department of 
            Biology, University of Virginia, Charlottesville, VA 22904, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    301..425
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        450..480
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             534..1190
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1535..1837
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1881..2005)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2017..2425
                     /label=partial GUS fragment
                     /note="partial GUS fragment"
     protein_bind    2441..2565
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2609..2911)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3256..3912)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3966..3996)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4021..4145)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4298..4642)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5074..5090)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5293..5317
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6617..7243
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7680..8744
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8813..9007
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9351..9491
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9677..10265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10355..11146)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11571..11595
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11673..11847)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11890..12912)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12980..13657)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    13848..13869
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13884..13914
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13922..13938
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13946..13962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
       61 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      121 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      181 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      241 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg gcggccgctc tagaactagg
      301 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      361 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      421 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      481 tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga
      541 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      601 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      661 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      721 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      781 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      841 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      901 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      961 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1021 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1081 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     1141 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
     1201 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     1261 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     1321 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1381 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1441 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1501 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     1561 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1621 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1681 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1741 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1801 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1861 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1921 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1981 gttcagcttt cttgtacaaa gtggttcgat aattccttaa ttaagatgtc acgccgtatg
     2041 ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga
     2101 ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc
     2161 atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc acgccgaaca
     2221 cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg
     2281 ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac
     2341 aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct
     2401 ggcaaccggg tgaaggttat ctctggcgcg ccaagctatc accactttgt acaagaaagc
     2461 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     2521 acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact
     2581 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
     2641 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
     2701 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
     2761 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
     2821 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
     2881 ctcttttata ggtgtaaacc ttaaactgca tttcaccagt ccctgttctc gtcagcaaaa
     2941 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
     3001 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
     3061 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
     3121 tacgctgctt catagcacac ctctttttga catacttcgg gtatacatat cagtatatat
     3181 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
     3241 ggatccacgc gtttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     3301 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
     3361 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     3421 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     3481 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     3541 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     3601 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     3661 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     3721 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     3781 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     3841 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     3901 ttttttctcc attttagctt ccttagctcc tgaaaatctc gccggatcct aactcaaaat
     3961 ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgcggccgc
     4021 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4081 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa
     4141 cttgtgataa ttcgagccta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg
     4201 ctttaaaaga aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt
     4261 cgtttgtttt gtatatgttg tgttgagaat tctcgaggtc ctctccaaat gaaatgaact
     4321 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     4381 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     4441 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc
     4501 aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac
     4561 tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat
     4621 taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt
     4681 tttggagtag acaagtgtgt cgtgctccac catgttatca catcaatcca cttgctttga
     4741 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     4801 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
     4861 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
     4921 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
     4981 gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat
     5041 gttgacctgc aggaattcga tatcaagctt ggcactggcc gtcgttttac aacgtcgtga
     5101 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5161 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5221 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     5281 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     5341 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     5401 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     5461 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     5521 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     5581 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     5641 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     5701 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     5761 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     5821 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     5881 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     5941 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     6001 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     6061 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     6121 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     6181 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     6241 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     6301 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     6361 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     6421 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     6481 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     6541 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     6601 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     6661 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     6721 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     6781 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     6841 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     6901 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     6961 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     7021 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     7081 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     7141 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     7201 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     7261 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     7321 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     7381 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     7441 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     7501 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     7561 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     7621 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     7681 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     7741 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     7801 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     7861 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     7921 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     7981 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     8041 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     8101 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     8161 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     8221 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     8281 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     8341 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     8401 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     8461 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     8521 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     8581 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     8641 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     8701 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     8761 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     8821 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     8881 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     8941 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     9001 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     9061 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     9121 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     9181 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     9241 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9301 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     9361 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     9421 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9481 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10081 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10321 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
    10381 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    10441 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    10501 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    10561 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    10621 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10681 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10741 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10801 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10861 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    10921 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    10981 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    11041 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    11101 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    11161 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    11221 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    11281 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    11341 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    11401 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    11461 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    11521 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11581 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    11641 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
    11701 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
    11761 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
    11821 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat ccggtcggca
    11881 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
    11941 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
    12001 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
    12061 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
    12121 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
    12181 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
    12241 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
    12301 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
    12361 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
    12421 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
    12481 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
    12541 cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc
    12601 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
    12661 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
    12721 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
    12781 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
    12841 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
    12901 aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga gatagatttg
    12961 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
    13021 aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
    13081 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    13141 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
    13201 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
    13261 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
    13321 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    13381 gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg
    13441 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    13501 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    13561 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    13621 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    13681 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
    13741 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    13801 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    13861 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    13921 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cg
//
