LOCUS       pHY300PLK.        4870 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Escherichia coli-Bacillus subtilis shuttle vector with ampicillin 
            and tetracycline resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pHY300PLK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   TaKaRa
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(103..1476)
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(1686..2687)
                     /label=repB
                     /note="RepB replication protein"
     promoter        3184..3288
                     /label=AmpR promoter
     CDS             3289..4146
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4314..4858
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagcttctag agatctgcag gtcgacggat ccccgggaat tcctgttata aaaaaaggat
       61 caattttgaa ctctctccca aagttgatcc cttaacgatt tagaaatccc tttgagaatg
      121 tttatataca ttcaaggtaa ccagccaact aatgacaatg attcctgaaa aaagtaataa
      181 caaattacta tacagataag ttgactgatc aacttccata ggtaacaacc tttgatcaag
      241 taagggtatg gataataaac cacctacaat tgcaatacct gttccctctg ataaaaagct
      301 ggtaaagtta agcaaactca ttccagcacc agcttcctgc tgtttcaagc tacttgaaac
      361 aattgttgat ataactgttt tggtgaacga aagcccacct aaaacaaata cgattataat
      421 tgtcatgaac catgatgttg tttctaaaag aaaggaagca gttaaaaagc taacagaaag
      481 aaatgtaact ccgatgttta acacgtataa aggacctctt ctatcaacaa gtatcccacc
      541 aatgtagccg aaaataatga cactcattgt tccagggaaa ataattacac ttccgatttc
      601 ggcagtactt agctggtgaa catctttcat catataagga accatagaga caaaccctgc
      661 tactgttcca aatataattc ccccacaaag aactccaatc ataaaaggta tatttttccc
      721 taatccggga tcaacaaaag gatctgttac tttcctgata tgttttacaa atatcaggaa
      781 tgacagcacg ctaacgataa gaaaagaaat gctatatgat gttgtaaaca acataaaaaa
      841 tacaatgcct acagacatta gtataattcc tttgatatca aaatgacctt ttatccttac
      901 ttctttcttt aataatttca taagaaacgg aacagtgata attgttatca taggaatgag
      961 tagaagatag gaccaatgaa tataatgggc tatcattcca ccaatcgctg gaccgactcc
     1021 ttctcccatg gctactatcg atccaataag accaaatgct ttacccctat tttcctttgg
     1081 aatatagcgc gcaactacaa ccattacgag tgctggaaat gcagctgcac cagccccttg
     1141 aataaaacga gccataataa gtaaggaaaa gaaagaatgg ccaacaaacc caattaccga
     1201 cccgaaacaa tttattataa ttccaaatag gagtaacctt ttgatgccta attgatcaga
     1261 tagctttcca tatacagctg ttccaatgga aaaggttaac ataaaggctg tgttcaccca
     1321 gtttgtactc gcaggtggtt tattaaaatc atttgcaata tcaggtaatg agacgttcaa
     1381 aaccatttca tttaatacgc taaaaaaaga taaaatgcaa agccaaatta aaatttggtt
     1441 gtgtcgtaaa ttcgattgtg aataggatgt attcacattt caccctccaa taatgagggc
     1501 agacgtagtt tatagggtta atgatacgct tccctctttt aattgaaccc tgttacattc
     1561 attattcatt acacttcata attaattcct cctaaacttg attaaaacat tttaccacat
     1621 ataaactaag ttttaaattc agtatttcat cacttataca acaatatggc ccgtttgttg
     1681 aactactctt taataaaata atttttccgt tcccaattcc acattgcaat aatagaaaat
     1741 ccatcttcat cggctttttc gtcatcatct gtatgaatca aatcgccttc ttctgtgtca
     1801 tcaaggttta attttttatg tatttctttt aacaaaccac cataggagat taacctttta
     1861 cggtgtaaac cttcctccaa atcagacaaa cgtttcaaat tcttttcttc atcatcggtc
     1921 ataaaatccg tatcctttac aggatatttt gcagtttcgt caattgccga ttgtatatcc
     1981 gatttatatt tatttttcgg tcgaatcatt tgaactttta catttggatc atagtctaat
     2041 ttcattgcct ttttccaaaa ttgaatccat tgtttttgat tcacgtagtt ttctgtattc
     2101 ttaaaataag ttggttccac acataccaat acatgcatgt gctgattata agaattatct
     2161 ttattattta ttgtcacttc cgttgcacgc ataaaaccaa caagattttt attaattttt
     2221 ttatattgca tcattcggcg aaatccttga gccatatctg acaaactctt atttaattct
     2281 tcgccatcat aaacattttt aactgttaat gtgagaaaca accaacgaac tgttggcttt
     2341 tgtttaataa cttcagcaac aaccttttgt gactgaatgc catgtttcat tgctctcctc
     2401 cagttgcaca ttggacaaag cctggattta caaaaccaca ctcgatacaa ctttctttcg
     2461 cctgtttcac gattttgttt atactctaat atttcagcac aatcttttac tctttcagcc
     2521 tttttaaatt caagaatatg cagaagttca aagtaatcaa cattagcgat tttcttttct
     2581 ctccatggtc tcacttttcc actttttgtc ttgtccacta aaacccttga tttttcatct
     2641 gaataaatgc tactattagg acacataata ttaaaagaaa cccccatcta tttagttatt
     2701 tgtttggtca cttataactt taacagatgg ggtttttctg tgcaaccaat tttaagggtt
     2761 ttccaatact ttaaaacaca tacataccaa cacttcaacg cacctttcag caactaaaat
     2821 aaaaatgacg ttatttctat atgtatcaag ataagaaaga acaagttcaa aaccatcaaa
     2881 aaaagacacc ttttcaggtg ctttttttat tttataaact cattccctga tctcgacttc
     2941 gttctttttt tacctctcgg ttatgagtta gttcaaattc gttcttttta ggttctaaat
     3001 cgtgtttttc ttggaattgt gctgttttat cctttacctt gtctacaaac cccttaaaaa
     3061 cgtttttaaa ggcttttaag cgtctgtacg ttccttaagg aattattcct tagtgctttc
     3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3181 gtccgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     3541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4261 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat
     4321 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggaggt
     4381 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tgcaggagcg
     4441 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     4501 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     4561 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     4621 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     4681 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     4741 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4801 accactgatt tgagcgtcag atttcgtgat gcttgtcagg gggcggagcc tatggaaaaa
     4861 cgctttgccc
//
