LOCUS       62056_12610        5228 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             138..854
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      881..952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      968..995
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2042..3043)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        3540..3644
                     /label=AmpR promoter
     CDS             3645..4502
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4670..5215
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagcttctag aataaacatt tattttgtat atgacgagat aaagttagtt tattggataa
       61 acaaactaac tcaattaaga tagttgatgg ataaacttgt tcacttaaat caaaggggga
      121 aatgacaaat ggtccaaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      601 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagtaactc gagtaaggat ctccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgggatc cccgggaatt ctccgttaat
     1021 gcgccatgac agccatgata attactaata ctaggagaag ttaataaata cgtaaccaac
     1081 atgattaaca attattagag gtcatcgttc aaaatggtat gcgttttgac acatccacta
     1141 tatatccgtg tcgttctgtc cactcctgaa tcccattcca gaaattctct agcgattcca
     1201 gaagtttctc agagtcggaa agttgaccag acattacgaa ctggcacaga tggtcataac
     1261 ctgaaggaag atctgattgc ttaactgctt cagttaagac cgaagcgctc gtcgtataac
     1321 agatgcgatg atgcagacca atcaacatgg cacctgccat tgctacctgt acagtcaagg
     1381 atggtagaaa tgttgtcggt ccttgcacac gaatattacg ccatttgcct gcatattcaa
     1441 acagctcttc tacgataagg gcacaaatcg catcgtggaa cgtttgggct tctaccgatt
     1501 tagcagtttg atacactttc tctaagtatc cacctgaatc ataaatcggc aaaatagaga
     1561 aaaattgacc atgtgtaagc ggccaatctg attccacctg agatgcataa tctagtagaa
     1621 tctcttcgct atcaaaattc acttccacct tccactcacc ggttgtccat tcatggctga
     1681 actctgcttc ctctgttgac atgacacaca tcatctcaat atccgaatag ggcccatcag
     1741 tctgacgacc aagagagcca taaacaccaa tagccttaac atcatcccca tatttatcca
     1801 atattcgttc cttaatttca tgaacaatct tcattctttc ttctctagtc attattattg
     1861 gtccattcac tattctcatt cccttttcag ataattttag atttgctttt ctaaataaga
     1921 atatttggag agcaccgttc ttattcagct attaataact cgtcttccta agcatccttc
     1981 aatcctttta ataacaatta tagcatctaa tcttcaacaa actggcccgt ttgttgaact
     2041 actctttaat aaaataattt ttccgttccc aattccacat tgcaataata gaaaatccat
     2101 cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa
     2161 ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac cttttacggt
     2221 gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca tcggtcataa
     2281 aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt atatccgatt
     2341 tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag tctaatttca
     2401 ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct gtattcttaa
     2461 aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa ttatctttat
     2521 tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta atttttttat
     2581 attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc
     2641 catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt
     2701 taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt
     2761 tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg
     2821 tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt
     2881 taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc ttttctctcc
     2941 atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt tcatctgaat
     3001 aaatgctact attaggacac ataatattaa aagaaacccc catctattta gttatttgtt
     3061 tagtcactta taactttaac agatggggtt tttctgtgca accaatttta agggttttca
     3121 atactttaaa acacatacat accaacactt caacgcacct ttcagcaact aaaataaaaa
     3181 tgacgttatt tctatatgta tcaagataag aaagaacaag ttcaaaacca tcaaaaaaag
     3241 acaccttttc aggtgctttt tttattttat aaactcattc cctgatctcg acttcgttct
     3301 ttttttacct ctcggttatg agttagttca aattcgttct ttttaggttc taaatcgtgt
     3361 ttttcttgga attgtgctgt tttatccttt accttgtcta caaacccctt aaaaacgttt
     3421 ttaaaggctt ttaagccgtc tgtacgttcc ttaaggaatt attccttagt gctttctagg
     3481 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtcc
     3541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     3901 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3961 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4021 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4081 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4141 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     4201 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4261 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4321 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4381 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4441 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4501 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4561 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4621 gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt
     4681 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggaggttttt
     4741 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgca ggagcgcagt
     4801 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
     4861 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
     4921 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
     4981 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
     5041 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     5101 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     5161 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     5221 ctttgccc
//
