LOCUS       62056_12605        4259 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1073..2074)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        2571..2675
                     /label=AmpR promoter
     CDS             2676..3533
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3701..4246
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagcttctag agatctgcag gtcgacggat ccccgggaat tctccgttaa tgcgccatga
       61 cagccatgat aattactaat actaggagaa gttaataaat acgtaaccaa catgattaac
      121 aattattaga ggtcatcgtt caaaatggta tgcgttttga cacatccact atatatccgt
      181 gtcgttctgt ccactcctga atcccattcc agaaattctc tagcgattcc agaagtttct
      241 cagagtcgga aagttgacca gacattacga actggcacag atggtcataa cctgaaggaa
      301 gatctgattg cttaactgct tcagttaaga ccgaagcgct cgtcgtataa cagatgcgat
      361 gatgcagacc aatcaacatg gcacctgcca ttgctacctg tacagtcaag gatggtagaa
      421 atgttgtcgg tccttgcaca cgaatattac gccatttgcc tgcatattca aacagctctt
      481 ctacgataag ggcacaaatc gcatcgtgga acgtttgggc ttctaccgat ttagcagttt
      541 gatacacttt ctctaagtat ccacctgaat cataaatcgg caaaatagag aaaaattgac
      601 catgtgtaag cggccaatct gattccacct gagatgcata atctagtaga atctcttcgc
      661 tatcaaaatt cacttccacc ttccactcac cggttgtcca ttcatggctg aactctgctt
      721 cctctgttga catgacacac atcatctcaa tatccgaata gggcccatca gtctgacgac
      781 caagagagcc ataaacacca atagccttaa catcatcccc atatttatcc aatattcgtt
      841 ccttaatttc atgaacaatc ttcattcttt cttctctagt cattattatt ggtccattca
      901 ctattctcat tcccttttca gataatttta gatttgcttt tctaaataag aatatttgga
      961 gagcaccgtt cttattcagc tattaataac tcgtcttcct aagcatcctt caatcctttt
     1021 aataacaatt atagcatcta atcttcaaca aactggcccg tttgttgaac tactctttaa
     1081 taaaataatt tttccgttcc caattccaca ttgcaataat agaaaatcca tcttcatcgg
     1141 ctttttcgtc atcatctgta tgaatcaaat cgccttcttc tgtgtcatca aggtttaatt
     1201 ttttatgtat ttcttttaac aaaccaccat aggagattaa ccttttacgg tgtaaacctt
     1261 cctccaaatc agacaaacgt ttcaaattct tttcttcatc atcggtcata aaatccgtat
     1321 cctttacagg atattttgca gtttcgtcaa ttgccgattg tatatccgat ttatatttat
     1381 ttttcggtcg aatcatttga acttttacat ttggatcata gtctaatttc attgcctttt
     1441 tccaaaattg aatccattgt ttttgattca cgtagttttc tgtattctta aaataagttg
     1501 gttccacaca taccaataca tgcatgtgct gattataaga attatcttta ttatttattg
     1561 tcacttccgt tgcacgcata aaaccaacaa gatttttatt aattttttta tattgcatca
     1621 ttcggcgaaa tccttgagcc atatctgaca aactcttatt taattcttcg ccatcataaa
     1681 catttttaac tgttaatgtg agaaacaacc aacgaactgt tggcttttgt ttaataactt
     1741 cagcaacaac cttttgtgac tgaatgccat gtttcattgc tctcctccag ttgcacattg
     1801 gacaaagcct ggatttacaa aaccacactc gatacaactt tctttcgcct gtttcacgat
     1861 tttgtttata ctctaatatt tcagcacaat cttttactct ttcagccttt ttaaattcaa
     1921 gaatatgcag aagttcaaag taatcaacat tagcgatttt cttttctctc catggtctca
     1981 cttttccact ttttgtcttg tccactaaaa cccttgattt ttcatctgaa taaatgctac
     2041 tattaggaca cataatatta aaagaaaccc ccatctattt agttatttgt ttagtcactt
     2101 ataactttaa cagatggggt ttttctgtgc aaccaatttt aagggttttc aatactttaa
     2161 aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa atgacgttat
     2221 ttctatatgt atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa gacacctttt
     2281 caggtgcttt ttttatttta taaactcatt ccctgatctc gacttcgttc tttttttacc
     2341 tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg tttttcttgg
     2401 aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt tttaaaggct
     2461 tttaagccgt ctgtacgttc cttaaggaat tattccttag tgctttctag gttaatgtca
     2521 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtc cgcggaaccc
     2581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     2941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3181 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     3241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3661 cgttccactg agcgtcagac cccttaataa gatgatcttc ttgagatcgt tttggtctgc
     3721 gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggaggtttt tcgaaggttc
     3781 tctgagctac caactctttg aaccgaggta actggcttgc aggagcgcag tcaccaaaac
     3841 ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa
     3901 ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga
     3961 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     4021 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     4081 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     4141 gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     4201 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccc
//
