LOCUS       62056_12600        5872 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             317..1033
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(1103..2476)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(2686..3687)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        4184..4288
                     /label=AmpR promoter
     CDS             4289..5146
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5314..5859
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagcttctag agatctgcag gtcgacggat cctcctttgt ttatccaccg aactaagttg
       61 gtgttttttg aagcttgaat tagatattta aaagtatcat atctaatatt ataactaaat
      121 tttctaaaaa aaacattgaa ataaacattt attttgtata tgatgagata aagttagttt
      181 attggataaa caaactaact caattaagat agttgatgga taaacttgtt cacttaaatc
      241 aaagggggaa atgacaaatg gtccaaacta gtgatatcta aaaatcaaag ggggaaatga
      301 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      361 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      421 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      481 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      541 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      601 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      661 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      721 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      781 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      841 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      901 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      961 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1021 cgagctgtac aagtaagaat tcctgttata aaaaaaggat caattttgaa ctctctccca
     1081 aagttgatcc cttaacgatt tagaaatccc tttgagaatg tttatataca ttcaaggtaa
     1141 ccagccaact aatgacaatg attcctgaaa aaagtaataa caaattacta tacagataag
     1201 ttgactgatc aacttccata ggtaacaacc tttgatcaag taagggtatg gataataaac
     1261 cacctacaat tgcaatacct gttccctctg ataaaaagct ggtaaagtta agcaaactca
     1321 ttccagcacc agcttcctgc tgtttcaagc tacttgaaac aattgttgat ataactgttt
     1381 tggtgaacga aagcccacct aaaacaaata cgattataat tgtcatgaac catgatgttg
     1441 tttctaaaag aaaggaagca gttaaaaagc taacagaaag aaatgtaact ccgatgttta
     1501 acacgtataa aggacctctt ctatcaacaa gtatcccacc aatgtagccg aaaataatga
     1561 cactcattgt tccagggaaa ataattacac ttccgatttc ggcagtactt agctggtgaa
     1621 catctttcat catataagga accatagaga caaaccctgc tactgttcca aatataattc
     1681 ccccacaaag aactccaatc ataaaaggta tatttttccc taatccggga tcaacaaaag
     1741 gatctgttac tttcctgata tgttttacaa atatcaggaa tgacagcacg ctaacgataa
     1801 gaaaagaaat gctatatgat gttgtaaaca acataaaaaa tacaatgcct acagacatta
     1861 gtataattcc tttgatatca aaatgacctt ttatccttac ttctttcttt aataatttca
     1921 taagaaacgg aacagtgata attgttatca taggaatgag tagaagatag gaccaatgaa
     1981 tataatgggc tatcattcca ccaatcgctg gaccgactcc ttctcccatg gctactatcg
     2041 atccaataag accaaatgct ttacccctat tttcctttgg aatatagcgc gcaactacaa
     2101 ccattacgag tgctggaaat gcagctgcac cagccccttg aataaaacga gccataataa
     2161 gtaaggaaaa gaaagaatgg ccaacaaacc caattaccga cccgaaacaa tttattataa
     2221 ttccaaatag gagtaacctt ttgatgccta attgatcaga tagctttcca tatacagctg
     2281 ttccaatgga aaaggttaac ataaaggctg tgttcaccca gtttgtactc gcaggtggtt
     2341 tattaaaatc atttgcaata tcaggtaatg agacgttcaa aaccatttca tttaatacgc
     2401 taaaaaaaga taaaatgcaa agccaaatta aaatttggtt gtgtcgtaaa ttcgattgtg
     2461 aataggatgt attcacattt caccctccaa taatgagggc agacgtagtt tatagggtta
     2521 atgatacgct tccctctttt aattgaaccc tgttacattc attattcatt acacttcata
     2581 attaattcct cctaaacttg attaaaacat tttaccacat ataaactaag ttttaaattc
     2641 agtatttcat cacttataca acaatatggc ccgtttgttg aactactctt taataaaata
     2701 atttttccgt tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc
     2761 gtcatcatct gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg
     2821 tatttctttt aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa
     2881 atcagacaaa cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac
     2941 aggatatttt gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg
     3001 tcgaatcatt tgaactttta catttggatc atagtctaat ttcattgcct ttttccaaaa
     3061 ttgaatccat tgtttttgat tcacgtagtt ttctgtattc ttaaaataag ttggttccac
     3121 acataccaat acatgcatgt gctgattata agaattatct ttattattta ttgtcacttc
     3181 cgttgcacgc ataaaaccaa caagattttt attaattttt ttatattgca tcattcggcg
     3241 aaatccttga gccatatctg acaaactctt atttaattct tcgccatcat aaacattttt
     3301 aactgttaat gtgagaaaca accaacgaac tgttggcttt tgtttaataa cttcagcaac
     3361 aaccttttgt gactgaatgc catgtttcat tgctctcctc cagttgcaca ttggacaaag
     3421 cctggattta caaaaccaca ctcgatacaa ctttctttcg cctgtttcac gattttgttt
     3481 atactctaat atttcagcac aatcttttac tctttcagcc tttttaaatt caagaatatg
     3541 cagaagttca aagtaatcaa cattagcgat tttcttttct ctccatggtc tcacttttcc
     3601 actttttgtc ttgtccacta aaacccttga tttttcatct gaataaatgc tactattagg
     3661 acacataata ttaaaagaaa cccccatcta tttagttatt tgtttggtca cttataactt
     3721 taacagatgg ggtttttctg tgcaaccaat tttaagggtt ttccaatact ttaaaacaca
     3781 tacataccaa cacttcaacg cacctttcag caactaaaat aaaaatgacg ttatttctat
     3841 atgtatcaag ataagaaaga acaagttcaa aaccatcaaa aaaagacacc ttttcaggtg
     3901 ctttttttat tttataaact cattccctga tctcgacttc gttctttttt tacctctcgg
     3961 ttatgagtta gttcaaattc gttcttttta ggttctaaat cgtgtttttc ttggaattgt
     4021 gctgttttat cctttacctt gtctacaaac cccttaaaaa cgtttttaaa ggcttttaag
     4081 cgtctgtacg ttccttaagg aattattcct tagtgctttc taggttaatg tcatgataat
     4141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtccgcggaa cccctatttg
     4201 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4261 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4321 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4381 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4441 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4501 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4561 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4621 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4681 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4741 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4801 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     4861 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4921 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4981 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5041 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5101 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5161 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5221 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5281 ctgagcgtca gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat
     5341 ctcttgctct gaaaacgaaa aaaccgcctt gcagggaggt ttttcgaagg ttctctgagc
     5401 taccaactct ttgaaccgag gtaactggct tgcaggagcg cagtcaccaa aacttgtcct
     5461 ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag
     5521 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac
     5581 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     5641 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
     5701 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg
     5761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
     5821 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cc
//
